Abstract
WXP1 is an ethylene‐responsive element‐binding transcription factor that has been shown to lead to improved drought tolerance and increased wax accumulation when overexpressed in transgenic alfalfa under the control of the CaMV35S promoter. In this study, alfalfa was transformed with the WXP1 gene, driven by the epidermis‐specific promoter CER6. The transgenic CER6::WXP1 alfalfa plants and a previously tested CaMV35S::WXP1 line were subjected to water stress for 3 d and then rewatered. Gas exchange, chlorophyll fluorescence, relative water content, and water potential were measured. Compared with the controls, the transgenic lines showed higher net photosynthetic rate, transpiration rate, and stomatal conductance and higher efficiency of photosystem II, quantum yield of photosystem II, coefficient of photochemical quenching, and apparent electron transport rate under water stress and after rewatering. The transgenic lines also showed higher relative water content and leaf water potential under water deficit conditions. The growth and development of CER6::WXP1 plants were normal, and the plants showed enhanced drought tolerance. Gas exchange and chlorophyll fluorescence data provided consistent evidence that the WXP1 transgenic lines experienced less damage under water stress conditions. This study revealed that increased wax accumulation did not have a negative impact on photosynthesis. The physiological analyses indicated that WXP1 is involved not only in wax biosynthesis but also in other physiological responses associated with enhanced drought tolerance that warrant further investigation.
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Claims 10
Claim · model-written summary
Transgenic WXP1 alfalfa showed higher net photosynthetic rate, transpiration rate, and stomatal conductance than controls under water stress and after rewatering.
- Taxon
- alfalfa
Medicago sativat0 - Material
- transgenic linese0
- Treatment
- water stresse0
- Outcome
- net photosynthetic rate, transpiration rate, and stomatal conductancee0
- Direction
- highere0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“Compared with the controls, the transgenic lines showed higher net photosynthetic rate, transpiration rate, and stomatal conductance and higher efficiency of photosystem II, quantum yield of photosystem II, coefficient of photochemical quenching, and apparent electron transport rate under water stress and after rewatering.”
Claim · model-written summary
Transgenic WXP1 alfalfa showed higher efficiency of photosystem II, quantum yield of photosystem II, coefficient of photochemical quenching than controls under water stress and after rewatering.
- Taxon
- alfalfaMedicago sativat0
- Material
- transgenic linese0
- Treatment
- water stresse0
- Outcome
- efficiency of photosystem II, quantum yield of photosystem II, coefficient of photochemical quenchinge0
- Direction
- highere0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“Compared with the controls, the transgenic lines showed higher net photosynthetic rate, transpiration rate, and stomatal conductance and higher efficiency of photosystem II, quantum yield of photosystem II, coefficient of photochemical quenching, and apparent electron transport rate under water stress and after rewatering.”
Claim · model-written summary
Transgenic WXP1 alfalfa showed higher apparent electron transport rate than controls under water stress and after rewatering.
- Taxon
- alfalfaMedicago sativat0
- Material
- transgenic linese0
- Treatment
- water stresse0
- Outcome
- apparent electron transport ratee0
- Direction
- highere0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“Compared with the controls, the transgenic lines showed higher net photosynthetic rate, transpiration rate, and stomatal conductance and higher efficiency of photosystem II, quantum yield of photosystem II, coefficient of photochemical quenching, and apparent electron transport rate under water stress and after rewatering.”
Claim · model-written summary
Transgenic lines had higher relative water content under water deficit.
- Taxon
- alfalfaMedicago sativat0
- Material
- transgenic linese0
- Outcome
- relative water contente0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“The transgenic lines also showed higher relative water content and leaf water potential under water deficit conditions.”
Claim · model-written summary
Transgenic lines had higher leaf water potential under water deficit.
- Taxon
- alfalfaMedicago sativat0
- Material
- transgenic linese0
- Outcome
- leaf water potentiale0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“The transgenic lines also showed higher relative water content and leaf water potential under water deficit conditions.”
Claim · model-written summary
CER6::WXP1 plants had normal growth and development.
- Taxon
- alfalfaMedicago sativat0
- Material
- CER6::WXP1 plantse0
- Outcome
- growth and developmente0
- Direction
- normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“The growth and development of CER6::WXP1 plants were normal, and the plants showed enhanced drought tolerance.”
Claim · model-written summary
CER6::WXP1 plants showed enhanced drought tolerance.
- Taxon
- alfalfaMedicago sativat0
- Material
- CER6::WXP1 plantse0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“The growth and development of CER6::WXP1 plants were normal, and the plants showed enhanced drought tolerance.”
Claim · model-written summary
WXP1 transgenic lines experienced less damage under water stress.
- Taxon
- alfalfaMedicago sativat0
- Material
- WXP1 transgenic linese0
- Treatment
- water stresse0
- Outcome
- damagee0
- Direction
- lesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“Gas exchange and chlorophyll fluorescence data provided consistent evidence that the WXP1 transgenic lines experienced less damage under water stress conditions.”
Claim · model-written summary
Increased wax accumulation did not negatively affect photosynthesis.
- Taxon
- alfalfaMedicago sativat0
- Compound
- waxe0
- Outcome
- photosynthesise0
- Direction
- did not have a negative impacte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“This study revealed that increased wax accumulation did not have a negative impact on photosynthesis.”
Claim · model-written summary
WXP1 was implicated in wax biosynthesis and other responses linked to drought tolerance.
- Taxon
- alfalfaMedicago sativat0
- Gene
- WXP1e0
- Outcome
- wax biosynthesise0
- Qualifier
- other physiological responses associated with enhanced drought tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa (Medicago sativa)”
e0“The physiological analyses indicated that WXP1 is involved not only in wax biosynthesis but also in other physiological responses associated with enhanced drought tolerance that warrant further investigation.”