An efficient regeneration system for peanut: Somatic embryogenesis from embryonic leaflets

Claim · model-written summary

Huayu 23 gave the highest somatic embryo formation frequency with 5 mg/L 2,4-D (76.1%) in peanut.

Taxon

peanutArachis hypogaeae0

Material

Huayu 23e2

Organ / stage

embryonic leafletse1

Treatment

5 mg L -1 2,4-De2

Outcome

frequency of somatic embryo formatione2

Direction

higheste2

Value

76.1%e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e1

“Somatic embryogenesis was induced from embryonic leaflets on MSB5 medium”

e2

“the frequency of somatic embryo formation was highest with 5 mg L -1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L -1 2,4-D for cultivar Huayu 20 (~ 79.5%), or 10 mg L -1 2,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively)”

Claim · model-written summary

Huayu 20 gave the highest somatic embryo formation frequency with 5 or 10 mg/L 2,4-D (about 79.5%) in peanut.

Taxon

peanutArachis hypogaeae0

Material

Huayu 20e2

Treatment

5 or 10 mg L -1 2,4-De2

Outcome

frequency of somatic embryo formatione2

Direction

higheste2

Value

~ 79.5%e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e2

“the frequency of somatic embryo formation was highest with 5 mg L -1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L -1 2,4-D for cultivar Huayu 20 (~ 79.5%), or 10 mg L -1 2,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively)”

Claim · model-written summary

Huayu 22 gave the highest somatic embryo formation frequency with 10 mg/L 2,4-D (92.9%) in peanut.

Taxon

peanutArachis hypogaeae0

Material

Huayu 22e2

Treatment

10 mg L -1 2,4-De2

Outcome

frequency of somatic embryo formatione2

Direction

higheste2

Value

92.9e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e2

“the frequency of somatic embryo formation was highest with 5 mg L -1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L -1 2,4-D for cultivar Huayu 20 (~ 79.5%), or 10 mg L -1 2,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively)”

Claim · model-written summary

Huayu 25 gave the highest somatic embryo formation frequency with 10 mg/L 2,4-D (91.3%) in peanut.

Taxon

peanutArachis hypogaeae0

Material

Huayu 25e2

Treatment

10 mg L -1 2,4-De2

Outcome

frequency of somatic embryo formatione2

Direction

higheste2

Value

91.3e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e2

“the frequency of somatic embryo formation was highest with 5 mg L -1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L -1 2,4-D for cultivar Huayu 20 (~ 79.5%), or 10 mg L -1 2,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively)”

Claim · model-written summary

Luhua 11 gave the highest somatic embryo formation frequency with 10 mg/L 2,4-D (92.3%) in peanut.

Taxon

peanutArachis hypogaeae0

Material

Luhua 11e2

Treatment

10 mg L -1 2,4-De2

Outcome

frequency of somatic embryo formatione2

Direction

higheste2

Value

92.3%e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e2

“the frequency of somatic embryo formation was highest with 5 mg L -1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L -1 2,4-D for cultivar Huayu 20 (~ 79.5%), or 10 mg L -1 2,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively)”

Claim · model-written summary

Plantlet conversion of somatic embryos was highest with 6-benzylaminopurine (74.6–93.4%) in peanut.

Taxon

peanutArachis hypogaeae0

Treatment

6-benzylaminopurinee3

Outcome

conversion of somatic embryos into plantletse3

Direction

higheste3

Value

74.6–93.4%e3

Comparator

thidiazurone3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e3

“conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6–93.4% conversion) and next highest with thidiazuron (35.6–78.2%, depending on concentration) but was substantially lower with gibberellic acid (0–24.6%, depending on concentration), abscisic acid (0–3.9%, depending on concentration), or the control (without growth regulators; 3.2–23.8%)”

Claim · model-written summary

Thidiazuron gave the next highest plantlet conversion (35.6–78.2%) in peanut.

Taxon

peanutArachis hypogaeae0

Treatment

thidiazurone3

Outcome

conversion of somatic embryos into plantletse3

Direction

next higheste3

Value

35.6–78.2%e3

Comparator

6-benzylaminopurinee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e3

“conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6–93.4% conversion) and next highest with thidiazuron (35.6–78.2%, depending on concentration) but was substantially lower with gibberellic acid (0–24.6%, depending on concentration), abscisic acid (0–3.9%, depending on concentration), or the control (without growth regulators; 3.2–23.8%)”

Claim · model-written summary

Gibberellic acid gave substantially lower plantlet conversion (0–24.6%) than BAP in peanut.

Taxon

peanutArachis hypogaeae0

Treatment

gibberellic acide3

Outcome

conversion of somatic embryos into plantletse3

Direction

substantially lowere3

Value

0–24.6%e3

Comparator

6-benzylaminopurinee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e3

“conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6–93.4% conversion) and next highest with thidiazuron (35.6–78.2%, depending on concentration) but was substantially lower with gibberellic acid (0–24.6%, depending on concentration), abscisic acid (0–3.9%, depending on concentration), or the control (without growth regulators; 3.2–23.8%)”

Claim · model-written summary

Abscisic acid gave substantially lower plantlet conversion (0–3.9%) than BAP in peanut.

Taxon

peanutArachis hypogaeae0

Treatment

abscisic acide3

Outcome

conversion of somatic embryos into plantletse3

Direction

substantially lowere3

Value

0–3.9%e3

Comparator

6-benzylaminopurinee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e3

“conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6–93.4% conversion) and next highest with thidiazuron (35.6–78.2%, depending on concentration) but was substantially lower with gibberellic acid (0–24.6%, depending on concentration), abscisic acid (0–3.9%, depending on concentration), or the control (without growth regulators; 3.2–23.8%)”

Claim · model-written summary

Medium without growth regulators gave substantially lower plantlet conversion (3.2–23.8%) than BAP in peanut.

Taxon

peanutArachis hypogaeae0

Treatment

without growth regulatorse3

Outcome

conversion of somatic embryos into plantletse3

Direction

substantially lowere3

Value

3.2–23.8%e3

Comparator

6-benzylaminopurinee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e3

“conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6–93.4% conversion) and next highest with thidiazuron (35.6–78.2%, depending on concentration) but was substantially lower with gibberellic acid (0–24.6%, depending on concentration), abscisic acid (0–3.9%, depending on concentration), or the control (without growth regulators; 3.2–23.8%)”

Claim · model-written summary

Of 33 regenerated Huayu 23 plantlets grafted and transplanted into the field, 31 grew to maturity and produced viable seeds.

Taxon

peanutArachis hypogaeae0

Material

Huayu 23e4

Treatment

cleft grafted onto germinated seedlingse4

Outcome

grew to maturity and produced viable seedse4

Value

31e4

Setting

into the fielde4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“developed for five peanut cultivars”

e4

“When 33 regenerated Huayu 23 plantlets were cleft grafted onto germinated seedlings and transplanted into the field, 31 grew to maturity and produced viable seeds”