Abstract
The regulatory role of thidiazuron (TDZ) and explant factors in imparting somatic embryogenic potential was assessed in relation to endogenous growth regulator levels in peanut ( Arachis hypogaea L. cv. Tango). TDZ induced somatic embryogenesis over a range of concentrations (0.5 to 10 μ M ). Culture of seedlings for just 2 days on TDZ‐supplemented medium was sufficient to induce somatic embryogenesis. Seedling age and retention or removal of cotyledons during culture influenced morphogenic potential significantly. The younger the seedlings, the better was the embryogenic response to TDZ treatment. The embryogenic potential of seedlings was limited for explants with no cotyledons and they did not respond to increasing levels of TDZ. In contrast, retention of at least one or both the cotyledons resulted in increased response to TDZ. Endogenous levels of cytokinins, auxins and abscisic acid were influenced by TDZ treatment, while TDZ itself was detected only in the cotyledons. The cytokinin N 6 ‐(2‐iso‐pentenyl)adenine (2iP) fluctuated on a 10‐day cycle in the cotyledons; TDZ treatment suppressed this change and caused an overall decrease in the pool of available 2iP in the tissue. It appeared that TDZ induced somatic embryogenesis in peanut by influencing endogenous levels of both auxin and cytokinins.
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Claims 9
Claim · model-written summary
TDZ induced somatic embryogenesis in peanut over a range of concentrations from 0.5 to 10 μM.
- Taxon
- peanut
Arachis hypogaeat0 - Treatment
- TDZe1
- Outcome
- somatic embryogenesise1
- Value
- 0.5 to 10 μ Me1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“TDZ induced somatic embryogenesis over a range of concentrations (0.5 to 10 μ M )”
Claim · model-written summary
Culture of seedlings for just 2 days on TDZ-supplemented medium was sufficient to induce somatic embryogenesis.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- Culture of seedlings for just 2 days on TDZ‐supplemented mediume1
- Outcome
- somatic embryogenesise1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“Culture of seedlings for just 2 days on TDZ‐supplemented medium was sufficient to induce somatic embryogenesis”
Claim · model-written summary
Younger seedlings gave a better embryogenic response to TDZ treatment.
- Taxon
- peanutArachis hypogaeat0
- Organ / stage
- seedlingse1
- Treatment
- TDZ treatmente1
- Outcome
- embryogenic responsee1
- Direction
- bettere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“The younger the seedlings, the better was the embryogenic response to TDZ treatment”
Claim · model-written summary
Seedling explants with no cotyledons had limited embryogenic potential and did not respond to increasing TDZ levels.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- explants with no cotyledonse1
- Outcome
- embryogenic potentiale1
- Direction
- limitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“The embryogenic potential of seedlings was limited for explants with no cotyledons and they did not respond to increasing levels of TDZ”
Claim · model-written summary
Endogenous levels of cytokinins, auxins and abscisic acid were influenced by TDZ treatment.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- TDZ treatmente1
- Compound
- cytokinins, auxins and abscisic acide1
- Outcome
- Endogenous levelse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“Endogenous levels of cytokinins, auxins and abscisic acid were influenced by TDZ treatment”
Claim · model-written summary
TDZ itself was detected only in the cotyledons.
- Taxon
- peanutArachis hypogaeat0
- Organ / stage
- cotyledonse1
- Compound
- TDZe1
- Outcome
- detectede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“TDZ itself was detected only in the cotyledons”
Claim · model-written summary
The cytokinin 2iP fluctuated on a 10-day cycle in the cotyledons.
- Taxon
- peanutArachis hypogaeat0
- Organ / stage
- cotyledonse1
- Outcome
- 2iPe1
- Direction
- fluctuatede1
- Qualifier
- 10‐day cyclee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“The cytokinin N 6 ‐(2‐iso‐pentenyl)adenine (2iP) fluctuated on a 10‐day cycle in the cotyledons”
Claim · model-written summary
TDZ treatment suppressed the 2iP fluctuation and caused an overall decrease in the pool of available 2iP.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- TDZ treatmente1
- Outcome
- pool of available 2iPe1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“TDZ treatment suppressed this change and caused an overall decrease in the pool of available 2iP in the tissue”
Claim · model-written summary
TDZ appears to induce somatic embryogenesis in peanut by influencing endogenous levels of auxin and cytokinins.
- Taxon
- peanutArachis hypogaeae1
- Compound
- auxin and cytokininse1
- Outcome
- somatic embryogenesise1
- Qualifier
- by influencing endogenous levels of both auxin and cytokininse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“somatic embryogenesis in intact seedlings of peanut”
e1“It appeared that TDZ induced somatic embryogenesis in peanut by influencing endogenous levels of both auxin and cytokinins”
The same questions in other papers
Thidiazuron · growth · Arachis hypogaea (peanut)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
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See all 6 papers →in vitro · growth · Arachis hypogaea (peanut)Positive and negative results
40 papers in the same direction as this paper · 3 in the opposite direction
- positive
- no effect
- no effect
40 papers more in this group
See all 44 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.