Abstract
Plant tissue culture and novel breeding techniques such as genome editing in the economically important crop Arachis hypogaea constitute potential for genetic improvement. Therefore, in this study, high-frequency in vitro regeneration via formation of adventitious shoots was described for different A. hypogaea lines. The leaflets of the primary leaves of 5-day-old seedlings were cultured for 16 weeks on Murashige and Skoog’s medium supplemented with equimolar concentrations (22.19 µM) of the cytokinins 6-benzylaminopurine (BAP); thidiazuron (TDZ); zeatin (Zea) or meta-topolin (MT), which was reduced to 7.40 µM after eight weeks plus 2.3 µM kinetin. Highest shoot regeneration percentages of 89.1% and 81.3% for BAP and TDZ containing media, respectively, were achieved in the line ’Jimmy’s pride’ with the maximum shoot number obtained on BAP. The regeneration percentages for three further lines were lower than 50%. Furthermore, the effect of different light qualities, applied using LEDs, and compared to fluorescent lamps was investigated. Shoot regeneration percentages did not improve significantly under the different LEDs, instead a pronounced effect on the regeneration efficiency of the culture temperature was observed which differed among the light treatments. However, fluorescent lamps can be replaced by red or blue LEDs without negative effects on shoot regeneration.
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Claims 8
Claim · model-written summary
High-frequency in vitro regeneration via adventitious shoots was described for different peanut lines.
- Taxon
- Arachis hypogaea
Arachis hypogaeat0 - Outcome
- high-frequency in vitro regeneration via formation of adventitious shootse1
- Setting
- in vitroe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e1“high-frequency in vitro regeneration via formation of adventitious shoots was described for different A. hypogaea lines”
Claim · model-written summary
BAP gave the highest shoot regeneration percentage of 89.1% in the line Jimmy’s pride.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Material
- Jimmy’s pridee2
- Treatment
- BAPe2
- Outcome
- shoot regeneration percentagese2
- Direction
- Higheste2
- Value
- 89.1%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e2“Highest shoot regeneration percentages of 89.1% and 81.3% for BAP and TDZ containing media, respectively, were achieved in the line ’Jimmy’s pride’”
Claim · model-written summary
TDZ gave the highest shoot regeneration percentage of 81.3% in the line Jimmy’s pride.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Material
- Jimmy’s pridee2
- Treatment
- TDZe2
- Outcome
- shoot regeneration percentagese2
- Direction
- Higheste2
- Value
- 81.3%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e2“Highest shoot regeneration percentages of 89.1% and 81.3% for BAP and TDZ containing media, respectively, were achieved in the line ’Jimmy’s pride’”
Claim · model-written summary
BAP gave the maximum shoot number in the line Jimmy’s pride.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- BAPe3
- Outcome
- shoot numbere3
- Direction
- maximume3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e3“with the maximum shoot number obtained on BAP”
Claim · model-written summary
Regeneration percentages for three further peanut lines were lower than 50%.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Outcome
- regeneration percentagese4
- Value
- lower than 50%e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e4“The regeneration percentages for three further lines were lower than 50%”
Claim · model-written summary
LED light did not significantly improve shoot regeneration percentages compared with fluorescent lamps.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- LEDse6
- Outcome
- shoot regeneration percentagese5
- Direction
- did not improvee5
- Comparator
- fluorescent lampse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e5“Shoot regeneration percentages did not improve significantly under the different LEDs”
e6“applied using LEDs, and compared to fluorescent lamps”
Claim · model-written summary
Culture temperature had a pronounced effect on regeneration efficiency that differed among the light treatments.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- culture temperaturee7
- Outcome
- regeneration efficiencye7
- Qualifier
- differed among the light treatmentse7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e7“a pronounced effect on the regeneration efficiency of the culture temperature was observed which differed among the light treatments”
Claim · model-written summary
The authors conclude that red or blue LEDs can replace fluorescent lamps without negative effects on shoot regeneration.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- red or blue LEDse7
- Outcome
- shoot regeneratione7
- Comparator
- fluorescent lampse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“peanut (Arachis hypogaea)”
e7“fluorescent lamps can be replaced by red or blue LEDs without negative effects on shoot regeneration”
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