Abstract
Summary. Treatment of excised marrow (Cucurbita pepo L., zucchini) cotyledons in darkness with 45 µM BA caused a marked stimulation of the growth accompanied by a strong decrease in cotyledons relative dry weight. PMSF – a well known inhibitor of both thiol- and serine-type proteases, present in the aqueous solutions either alone or with the cytokinin, inhibited significantly the growth of cotyledons and hindered the decrease in cotyledons dry weight in all treatments. SDS-PAGE analysis of the protein profiles showed that PMSF suppressed the gradual decline in the quantity of the 20– 2 kDa polypeptide group and the low-molecular-weight polypeptide bands (below 15 kDa) both in the control and BA-treated cotyledons. On the other hand, the polypeptide bands migrating in the higher molecular weight zone of the profiles including the 97.4-kDa polypeptide and the LSU of Rubisco (55 kDa) decreased in quantity in the presence of PMSF. These results suggest that the inhibitory effect of PMSF on the breakdown of storage proteins in the excised marrow cotyledons may be due to an inhibition only of certain PMSF-sensitive proteases taking part in this process during germination.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
BA stimulated cotyledon growth.
- Taxon
- Cucurbita pepo L.
Cucurbita pepoe0 - Organ / stage
- cotyledonse0
- Treatment
- 45 µM BAe0
- Outcome
- growthe0
- Direction
- stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of excised marrow (Cucurbita pepo L., zucchini) cotyledons in darkness with 45 µM BA caused a marked stimulation of the growth”
Claim · model-written summary
BA decreased relative cotyledon dry weight.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- 45 µM BAe0
- Outcome
- cotyledons relative dry weighte0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with 45 µM BA caused a marked stimulation of the growth accompanied by a strong decrease in cotyledons relative dry weight.”
t0“CUCURBITA PEPO L.”
Claim · model-written summary
PMSF inhibited cotyledon growth with and without BA.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- PMSFe0
- Outcome
- growth of cotyledonse0
- Direction
- inhibitede0
- Qualifier
- either alone or with the cytokinine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PMSF – a well known inhibitor of both thiol- and serine-type proteases, present in the aqueous solutions either alone or with the cytokinin, inhibited significantly the growth of cotyledons”
t0“CUCURBITA PEPO L.”
Claim · model-written summary
PMSF hindered the decrease in cotyledon dry weight.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- PMSFe0
- Outcome
- cotyledons dry weighte0
- Direction
- hindered the decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PMSF – a well known inhibitor of both thiol- and serine-type proteases, present in the aqueous solutions either alone or with the cytokinin, inhibited significantly the growth of cotyledons and hindered the decrease in cotyledons dry weight in all treatments.”
t0“CUCURBITA PEPO L.”
Claim · model-written summary
PMSF suppressed decline of the 20–2 kDa polypeptide group.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- PMSFe0
- Outcome
- quantity of the 20– 2 kDa polypeptide groupe0
- Direction
- suppressed the gradual declinee0
- Comparator
- the control and BA-treated cotyledonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PMSF suppressed the gradual decline in the quantity of the 20– 2 kDa polypeptide group and the low-molecular-weight polypeptide bands (below 15 kDa) both in the control and BA-treated cotyledons.”
t0“CUCURBITA PEPO L.”
Claim · model-written summary
PMSF suppressed decline of low molecular weight polypeptides.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- PMSFe0
- Outcome
- low-molecular-weight polypeptide bands (below 15 kDa)e0
- Direction
- suppressed the gradual declinee0
- Comparator
- the control and BA-treated cotyledonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PMSF suppressed the gradual decline in the quantity of the 20– 2 kDa polypeptide group and the low-molecular-weight polypeptide bands (below 15 kDa) both in the control and BA-treated cotyledons.”
t0“CUCURBITA PEPO L.”
Claim · model-written summary
Higher molecular weight polypeptides decreased with PMSF.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- PMSFe0
- Outcome
- polypeptide bands migrating in the higher molecular weight zonee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the polypeptide bands migrating in the higher molecular weight zone of the profiles including the 97.4-kDa polypeptide and the LSU of Rubisco (55 kDa) decreased in quantity in the presence of PMSF.”
t0“CUCURBITA PEPO L.”
Claim · model-written summary
The authors suggested that PMSF inhibits storage protein breakdown through certain sensitive proteases.
- Taxon
- Cucurbita pepo L.Cucurbita pepot0
- Treatment
- PMSFe0
- Outcome
- breakdown of storage proteinse0
- Qualifier
- inhibition only of certain PMSF-sensitive proteasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibitory effect of PMSF on the breakdown of storage proteins in the excised marrow cotyledons may be due to an inhibition only of certain PMSF-sensitive proteases taking part in this process during germination.”
t0“CUCURBITA PEPO L.”
The same questions in other papers
N-benzyladenine · growth · Cucurbita pepoPositive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.