Abstract
The effects of white light, far-red light, and darkness on the transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1, were investigated. RNA was labeled with [alpha-32P]UTP in nuclei isolated from plants grown under different conditions of light and darkness and used to probe Southern blots and dot blots. The levels of small subunit mRNA synthesis were normalized to ribosomal RNA synthesis. We demonstrate that the SRS1 gene is transcribed at a rate 16- to 32-fold higher in plants grown in the light than in those grown in darkness. Transcription of the small subunit increased dramatically when plants grown in darkness were given 30 min to 6 h of light and then leveled off after 24 to 48 h of exposure. When light-grown seedlings were exposed to greater than 2 h of darkness, a gradual decrease in transcription was detected. This decrease in transcription reached basal dark-grown levels after 48 h of exposure to darkness. The increase in transcription in etiolated seedlings treated with white light for 15 min could be reduced to basal levels if the treatment was followed by treatment with far-red light for 15 min. In addition, transcription in ligh-grown seedlings was reduced to basal levels when plants were exposed to far-red light for 15 min. The transcription of this ribulose-1,5-biphosphate carboxylase small subunit gene is strongly positively regulated by white light, is negatively regulated by far-red light, and exhibits a classic phytochrome-linked response.
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Claims 8
Claim · model-written summary
SRS1 is transcribed 16- to 32-fold higher in plants grown in the light than in those grown in darkness
- Taxon
- soybean
Glycine maxe0 - Treatment
- grown in the lighte1
- Gene
- SRS1e1
- Outcome
- transcribed at a ratee1
- Direction
- highere1
- Value
- 16- to 32-folde1
- Comparator
- those grown in darknesse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e1“We demonstrate that the SRS1 gene is transcribed at a rate 16- to 32-fold higher in plants grown in the light than in those grown in darkness”
Claim · model-written summary
Small subunit transcription increased dramatically when dark-grown plants were given 30 min to 6 h of light
- Taxon
- soybeanGlycine maxe0
- Treatment
- 30 min to 6 h of lighte1
- Outcome
- Transcription of the small subunite1
- Direction
- increasede1
- Qualifier
- dramaticallye1
- Comparator
- plants grown in darknesse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e1“Transcription of the small subunit increased dramatically when plants grown in darkness were given 30 min to 6 h of light and then leveled off after 24 to 48 h of exposure”
Claim · model-written summary
Small subunit transcription leveled off after 24 to 48 h of light exposure
- Taxon
- soybeanGlycine maxe0
- Outcome
- Transcription of the small subunite1
- Direction
- leveled offe1
- Qualifier
- 24 to 48 h of exposuree1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e1“Transcription of the small subunit increased dramatically when plants grown in darkness were given 30 min to 6 h of light and then leveled off after 24 to 48 h of exposure”
Claim · model-written summary
A gradual decrease in transcription was detected when light-grown seedlings were exposed to more than 2 h of darkness
- Taxon
- soybeanGlycine maxe0
- Treatment
- greater than 2 h of darknesse2
- Outcome
- transcriptione2
- Direction
- decreasee2
- Qualifier
- graduale2
- Comparator
- light-grown seedlingse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e2“When light-grown seedlings were exposed to greater than 2 h of darkness, a gradual decrease in transcription was detected”
Claim · model-written summary
Transcription decrease reached basal dark-grown levels after 48 h of exposure to darkness
- Taxon
- soybeanGlycine maxe0
- Outcome
- transcriptione3
- Qualifier
- basal dark-grown levels after 48 h of exposure to darknesse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e3“This decrease in transcription reached basal dark-grown levels after 48 h of exposure to darkness”
Claim · model-written summary
The increase in transcription in etiolated seedlings after 15 min of white light could be reduced to basal levels by 15 min of far-red light
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- etiolated seedlingse4
- Treatment
- far-red light for 15 mine4
- Outcome
- increase in transcriptione4
- Direction
- reducede4
- Comparator
- white light for 15 mine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e4“The increase in transcription in etiolated seedlings treated with white light for 15 min could be reduced to basal levels if the treatment was followed by treatment with far-red light for 15 min”
Claim · model-written summary
Transcription in light-grown seedlings was reduced to basal levels when plants were exposed to 15 min of far-red light
- Taxon
- soybeanGlycine maxe0
- Treatment
- far-red light for 15 mine5
- Outcome
- transcriptione5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e5“transcription in ligh-grown seedlings was reduced to basal levels when plants were exposed to far-red light for 15 min”
Claim · model-written summary
Transcription of the small subunit gene is positively regulated by white light and negatively regulated by far-red light, a classic phytochrome-linked response
- Taxon
- soybeanGlycine maxe0
- Treatment
- white lighte6
- Outcome
- transcriptione6
- Qualifier
- classic phytochrome-linked responsee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1”
e6“The transcription of this ribulose-1,5-biphosphate carboxylase small subunit gene is strongly positively regulated by white light, is negatively regulated by far-red light, and exhibits a classic phytochrome-linked response”