Abstract
Exposure to temperatures exceeding the normal optimum levels, or heat stress (HS), constitutes an environmental disruption for plants, resulting in severe growth and development retardation. Here we show that loss of function of the Arabidopsis histone acetyltransferase GCN5 results in serious defects in terms of thermotolerance, and considerably impairs the transcriptional activation of HS-responsive genes. Notably, expression of several key regulators such as the HS transcription factors HSFA2 and HSFA3, Multiprotein Bridging Factor 1c (MBF1c) and UV-HYPERSENSITIVE 6 (UVH6) is down-regulated in the gcn5 mutant under HS compared with the wild-type. Chromatin immunoprecipitation (ChIP) assays indicated that GCN5 protein is enriched at the promoter regions of HSFA3 and UVH6 genes, but not in HSFA2 and MBF1c, and that GCN5 facilitates H3K9 and H3K14 acetylation, which are associated with HSFA3 and UVH6 activation under HS. Moreover, constitutive expression of UVH6 in the gcn5 mutant partially restores heat tolerance. Taken together, our data indicate that GCN5 plays a key role in the preservation of thermotolerance via versatile regulation in Arabidopsis. In addition, expression of the wheat TaGCN5 gene re-establishes heat tolerance in Arabidopsis gcn5 mutant plants, suggesting that GCN5-mediated thermotolerance may be conserved between Arabidopsis and wheat.
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Claims 9
Claim · model-written summary
Loss of GCN5 seriously impaired Arabidopsis thermotolerance.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- loss of functione0
- Gene
- GCN5e0
- Outcome
- thermotolerancee0
- Direction
- defectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of function of the Arabidopsis histone acetyltransferase GCN5 results in serious defects in terms of thermotolerance”
Claim · model-written summary
Loss of GCN5 impaired transcriptional activation of heat-responsive genes.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- loss of functione0
- Gene
- GCN5e0
- Outcome
- transcriptional activation of HS-responsive genese0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of function of the Arabidopsis histone acetyltransferase GCN5 results in serious defects in terms of thermotolerance, and considerably impairs the transcriptional activation of HS-responsive genes.”
Claim · model-written summary
HSFA2 and HSFA3 expression was down-regulated in heat-stressed gcn5 versus wild type.
- Taxon
- ArabidopsisArabidopsist0
- Material
- gcn5 mutante0
- Gene
- HSFA2 and HSFA3e0
- Outcome
- expressione0
- Direction
- down-regulatede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of several key regulators such as the HS transcription factors HSFA2 and HSFA3, Multiprotein Bridging Factor 1c (MBF1c) and UV-HYPERSENSITIVE 6 (UVH6) is down-regulated in the gcn5 mutant under HS compared with the wild-type.”
t0“Arabidopsis”
Claim · model-written summary
MBF1c and UVH6 expression was down-regulated in heat-stressed gcn5 versus wild type.
- Taxon
- ArabidopsisArabidopsist0
- Material
- gcn5 mutante0
- Gene
- MBF1ce0
- Outcome
- expressione0
- Direction
- down-regulatede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of several key regulators such as the HS transcription factors HSFA2 and HSFA3, Multiprotein Bridging Factor 1c (MBF1c) and UV-HYPERSENSITIVE 6 (UVH6) is down-regulated in the gcn5 mutant under HS compared with the wild-type.”
t0“Arabidopsis”
Claim · model-written summary
GCN5 was enriched at HSFA3 and UVH6 promoter regions.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GCN5e0
- Outcome
- promoter regions of HSFA3 and UVH6 genese0
- Direction
- enrichede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GCN5 protein is enriched at the promoter regions of HSFA3 and UVH6 genes”
t0“Arabidopsis”
Claim · model-written summary
GCN5 was not enriched at HSFA2 and MBF1c promoters.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HSFA2 and MBF1ce0
- Outcome
- not in HSFA2 and MBF1ce0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not in HSFA2 and MBF1c”
t0“Arabidopsis”
Claim · model-written summary
GCN5 facilitated H3K9 and H3K14 acetylation associated with HSFA3 and UVH6 activation under heat stress.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GCN5e0
- Outcome
- H3K9 and H3K14 acetylatione0
- Qualifier
- associated with HSFA3 and UVH6 activation under HSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GCN5 facilitates H3K9 and H3K14 acetylation, which are associated with HSFA3 and UVH6 activation under HS.”
t0“Arabidopsis”
Claim · model-written summary
Constitutive UVH6 expression partially restored heat tolerance in the gcn5 mutant.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- constitutive expression of UVH6e0
- Gene
- UVH6e0
- Outcome
- heat tolerancee0
- Direction
- restorese0
- Qualifier
- partiallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constitutive expression of UVH6 in the gcn5 mutant partially restores heat tolerance.”
t0“Arabidopsis”
Claim · model-written summary
Wheat TaGCN5 expression restored heat tolerance in Arabidopsis gcn5 plants.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- expression of the wheat TaGCN5 genee0
- Gene
- wheat TaGCN5e0
- Outcome
- heat tolerancee0
- Direction
- re-establishese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the wheat TaGCN5 gene re-establishes heat tolerance in Arabidopsis gcn5 mutant plants”
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See all 197 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.