Paper · 2008 · Indian Journal of Horticulture

Rapid clonal in vitro multiplication of Citrus jambhiri and Citrus karna

Ashutosh A. Murkute; Satyawati Sharma; Sarbjot Singh

7
claims
12
verbatim quotes
7
citations
3
research areas
✓
Open access

Abstract

Investigation was conducted to develop a rapid in vitro regeneration protocol for Citrus jambhiri and C. karna, two important rootstocks commonly used in India. Among different plant growth regulators, the treatment containing 1 mg/l BAP, 0.5 mg/l NAA and 40 mg/l adenine sulphate significantly improved culture responses including percentage shoot bud formation, days required for shoot initiation, shoot length and number of resultant shoots per explant. Rooting was achieved on ½MS basal medium supplemented with NAA and IBA either in combination or individually at different concentrations. Medium containing 0.5 mg/l IBA + 0.5 mg/l NAA gave maximum response in both. The hardening medium consisted of 2 coco-peat: 1 solrite in which the survival rates of hardened plants were 94.7% in C. jambhiri and 96.7% in C. karna after one month. Subsequently, 65.5 and 71.8% in C. jambhiri and C. karna respectively, survived ex vitro.

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Claims 7

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The BAP, NAA and adenine sulphate treatment significantly improved shoot formation, initiation, length and shoot number.

Taxon
Citrus jambhiri and Citrus karnaCitrus jambhiriCitrus karnat0
Treatment
1 mg/l BAP, 0.5 mg/l NAA and 40 mg/l adenine sulphatee0
Outcome
percentage shoot bud formation, days required for shoot initiation, shoot length and number of resultant shoots per explante0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗
Citrus karna
Citrus karna · TaxonA. Barra · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“1 mg/l BAP, 0.5 mg/l NAA and 40 mg/l adenine sulphate significantly improved culture responses including percentage shoot bud formation, days required for shoot initiation, shoot length and number of resultant shoots per explant”

✓ located verbatim in the source · abstract, characters 227–455 · show in text ↑
t0

“Citrus jambhiri and Citrus karna”

✓ located verbatim in the source · title, characters 40–72 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA and IBA supplements produced rooting.

Taxon
Citrus jambhiri and Citrus karnaCitrus jambhiriCitrus karnat0
Treatment
½MS basal medium supplemented with NAA and IBAe0
Outcome
Rooting was achievede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗
Citrus karna
Citrus karna · TaxonA. Barra · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Rooting was achieved on ½MS basal medium supplemented with NAA and IBA either in combination or individually at different concentrations”

✓ located verbatim in the source · abstract, characters 457–593 · show in text ↑
t0

“Citrus jambhiri and Citrus karna”

✓ located verbatim in the source · title, characters 40–72 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The combined 0.5 mg/l IBA and 0.5 mg/l NAA medium gave the maximum response in both rootstocks.

Taxon
Citrus jambhiri and Citrus karnaCitrus jambhiriCitrus karnat0
Treatment
0.5 mg/l IBA + 0.5 mg/l NAAe0
Outcome
maximum responsee0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗
Citrus karna
Citrus karna · TaxonA. Barra · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Medium containing 0.5 mg/l IBA + 0.5 mg/l NAA gave maximum response in both”

✓ located verbatim in the source · abstract, characters 595–670 · show in text ↑
t0

“Citrus jambhiri and Citrus karna”

✓ located verbatim in the source · title, characters 40–72 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Hardened C. jambhiri plants had 94.7% one-month survival in the coco-peat and solrite medium.

Taxon
C. jambhiriCitrus jambhirie0
Treatment
2 coco-peat: 1 solritee0
Outcome
survival rates of hardened plantse0
Value
94.7%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“2 coco-peat: 1 solrite in which the survival rates of hardened plants were 94.7% in C. jambhiri”

✓ located verbatim in the source · abstract, characters 706–801 · show in text ↑
t0

“Citrus jambhiri”

✓ located verbatim in the source · title, characters 40–55 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Hardened C. karna plants had 96.7% one-month survival.

Taxon
C. karnaCitrus karnae0
Outcome
survival rates of hardened plantse1
Value
96.7%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus karna
Citrus karna · TaxonA. Barra · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“96.7% in C. karna after one month”

✓ located verbatim in the source · abstract, characters 806–839 · show in text ↑
e1

“survival rates of hardened plants”

✓ located verbatim in the source · abstract, characters 742–775 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ex vitro survival was 65.5% for C. jambhiri.

Taxon
C. jambhiriCitrus jambhirie0
Outcome
survived ex vitroe0
Value
65.5e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“65.5 and 71.8% in C. jambhiri and C. karna respectively, survived ex vitro”

✓ located verbatim in the source · abstract, characters 855–929 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ex vitro survival was 71.8% for C. karna.

Taxon
C. karnaCitrus karnae0
Outcome
survived ex vitroe0
Value
71.8%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus karna
Citrus karna · TaxonA. Barra · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“65.5 and 71.8% in C. jambhiri and C. karna respectively, survived ex vitro”

✓ located verbatim in the source · abstract, characters 855–929 · show in text ↑

N-benzyladenine · growth · Citrus jambhiri (rough lemon)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

1-naphthaleneacetic acid · growth · Citrus jambhiri (rough lemon)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.