Paper · 2020 · Biotechnology Journal International

Preparation of Synthetic Seeds of Citrus jambhiri Using in vitro Regenerated Multiple Plantlets

Priyanka Sharma; Bidhan Roy

8
claims
14
verbatim quotes
7
citations
4
research areas
✓
Open access

Abstract

Biotechnological tools are useful for true-to-type propagation. Shoot tips encapsulation is potential for plant development from pre-existing meristematic tissue. MS medium fortified with 1 and 2 mg/L of BAP (6-bezylaminopurine) was found to be suitable for in vitro mass-multiplication of plantlets (10.18 and 13.05 plantlets/explant, respectively) of Citrus jambhiri from nodal segments. Nodal segments were more appropriate than the shoot tips for in vitro multiplication of plantlets. Synthetic seeds were prepared using 2.5% sodium alginate dropping into 3.0% CaCl2 solution. Maximum germination was recorded when beaded shoot tips were cultured on MS medium fortified with 1 and 2 mg/L of BAP (96.67 and 100.00%, respectively). However, the germination of synthetic seeds was found to be comparatively high than the earlier findings. The results support the use of encapsulated unipolar explants for synthetic seed preparation. These type of capsules could be useful in exchange of sterile material between laboratories, germplasm conservation and direct plant propagation.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with 1 mg/L BAP yielded 10.18 plantlets per explant.

Taxon
Citrus jambhiriCitrus jambhirie0
Organ / stage
nodal segmentse0
Treatment
1e0
Outcome
mass-multiplication of plantletse0
Value
10.18e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“MS medium fortified with 1 and 2 mg/L of BAP (6-bezylaminopurine) was found to be suitable for in vitro mass-multiplication of plantlets (10.18 and 13.05 plantlets/explant, respectively) of Citrus jambhiri from nodal segments.”

✓ located verbatim in the source · abstract, characters 163–389 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with 2 mg/L BAP yielded 13.05 plantlets per explant.

Taxon
Citrus jambhiriCitrus jambhirie0
Organ / stage
nodal segmentse0
Treatment
2 mg/L of BAPe0
Outcome
mass-multiplication of plantletse0
Value
13.05 plantlets/explante0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“MS medium fortified with 1 and 2 mg/L of BAP (6-bezylaminopurine) was found to be suitable for in vitro mass-multiplication of plantlets (10.18 and 13.05 plantlets/explant, respectively) of Citrus jambhiri from nodal segments.”

✓ located verbatim in the source · abstract, characters 163–389 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nodal segments were more appropriate for plantlet multiplication than shoot tips.

Taxon
Citrus jambhiriCitrus jambhirit0
Treatment
Nodal segmentse0
Outcome
in vitro multiplication of plantletse0
Direction
more appropriatee0
Comparator
shoot tipse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Nodal segments were more appropriate than the shoot tips for in vitro multiplication of plantlets.”

✓ located verbatim in the source · abstract, characters 390–488 · show in text ↑
t0

“Citrus jambhiri”

✓ located verbatim in the source · title, characters 34–49 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with 1 mg/L BAP gave 96.67% germination of beaded shoot tips.

Taxon
Citrus jambhiriCitrus jambhirit0
Treatment
1e0
Outcome
germinatione0
Direction
Maximume0
Value
96.67e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum germination was recorded when beaded shoot tips were cultured on MS medium fortified with 1 and 2 mg/L of BAP (96.67 and 100.00%, respectively).”

✓ located verbatim in the source · abstract, characters 581–733 · show in text ↑
t0

“Citrus jambhiri”

✓ located verbatim in the source · title, characters 34–49 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with 2 mg/L BAP gave 100.00% germination of beaded shoot tips.

Taxon
Citrus jambhiriCitrus jambhirit0
Treatment
2 mg/L of BAPe0
Outcome
germinatione0
Direction
Maximume0
Value
100.00%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum germination was recorded when beaded shoot tips were cultured on MS medium fortified with 1 and 2 mg/L of BAP (96.67 and 100.00%, respectively).”

✓ located verbatim in the source · abstract, characters 581–733 · show in text ↑
t0

“Citrus jambhiri”

✓ located verbatim in the source · title, characters 34–49 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Synthetic seed germination was comparatively high relative to earlier findings.

Taxon
Citrus jambhiriCitrus jambhirit0
Outcome
germination of synthetic seedse0
Direction
comparatively highe0
Comparator
the earlier findingse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“However, the germination of synthetic seeds was found to be comparatively high than the earlier findings.”

✓ located verbatim in the source · abstract, characters 734–839 · show in text ↑
t0

“Citrus jambhiri”

✓ located verbatim in the source · title, characters 34–49 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The results support encapsulated unipolar explants for synthetic seed preparation.

Taxon
Citrus jambhiriCitrus jambhirit0
Outcome
use of encapsulated unipolar explants for synthetic seed preparatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The results support the use of encapsulated unipolar explants for synthetic seed preparation.”

✓ located verbatim in the source · abstract, characters 840–933 · show in text ↑
t0

“Citrus jambhiri”

✓ located verbatim in the source · title, characters 34–49 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors suggest capsules for sterile material exchange, germplasm conservation and direct propagation.

Taxon
Citrus jambhiriCitrus jambhirit0
Outcome
exchange of sterile material between laboratories, germplasm conservation and direct plant propagatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus jambhiri
Citrus jambhiri · TaxonTon Rulkens from Mozambique · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“These type of capsules could be useful in exchange of sterile material between laboratories, germplasm conservation and direct plant propagation.”

✓ located verbatim in the source · abstract, characters 934–1079 · show in text ↑
t0

“Citrus jambhiri”

✓ located verbatim in the source · title, characters 34–49 · show in text ↑

N-benzyladenine · growth · Citrus jambhiri (rough lemon)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.