Abstract
2-Cys peroxiredoxins (2-CPs) function in the removal of hydrogen peroxide and lipid peroxides but their precise roles in the induction of autophagy have not been characterized. Here we show that heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants. Virus-induced gene silencing of the tomato peroxiredoxin genes 2-CP1, 2-CP2, and 2-CP1/2 resulted in an increased sensitivity of tomato plants to heat stress. Silencing 2-CP2 or 2-CP1/2 increased the levels of transcripts associated with ascorbate biosynthesis but had no effect on the glutathione pool in the absence of stress. However, the heat-induced accumulation of transcripts associated with the water-water cycle was compromised by the loss of 2-CP1/2 functions. The transcript levels of autophagy-related genes ATG5 and ATG7 were higher in plants with impaired 2-CP1/2 functions, and the formation of autophagosomes increased, together with an accumulation of oxidized and insoluble proteins. Silencing of ATG5 or ATG7 increased the levels of 2-CP transcripts and protein but decreased heat stress tolerance. These results demonstrate that 2-CPs fulfil a pivotal role in heat stress tolerance in tomato, via interactions with ascorbate-dependent pathways and autophagy.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Heat stress led to simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, and of autophagosomes, in tomato.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- heat stresse1
- Outcome
- simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
Claim · model-written summary
Silencing of 2-CP1, 2-CP2 and 2-CP1/2 increased the sensitivity of tomato plants to heat stress.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Virus-induced gene silencing of the tomato peroxiredoxin genes 2-CP1, 2-CP2, and 2-CP1/2e2
- Outcome
- sensitivity of tomato plants to heat stresse2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e2“Virus-induced gene silencing of the tomato peroxiredoxin genes 2-CP1, 2-CP2, and 2-CP1/2 resulted in an increased sensitivity of tomato plants to heat stress”
Claim · model-written summary
Silencing 2-CP2 or 2-CP1/2 increased the levels of transcripts associated with ascorbate biosynthesis.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Silencing 2-CP2 or 2-CP1/2e3
- Outcome
- levels of transcripts associated with ascorbate biosynthesise3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e3“Silencing 2-CP2 or 2-CP1/2 increased the levels of transcripts associated with ascorbate biosynthesis but had no effect on the glutathione pool in the absence of stress”
Claim · model-written summary
Silencing 2-CP2 or 2-CP1/2 had no effect on the glutathione pool in the absence of stress.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Silencing 2-CP2 or 2-CP1/2e3
- Outcome
- glutathione poole3
- Direction
- no effecte3
- Qualifier
- in the absence of stresse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e3“Silencing 2-CP2 or 2-CP1/2 increased the levels of transcripts associated with ascorbate biosynthesis but had no effect on the glutathione pool in the absence of stress”
Claim · model-written summary
Loss of 2-CP1/2 function compromised the heat-induced accumulation of transcripts associated with the water-water cycle.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- loss of 2-CP1/2 functionse4
- Outcome
- heat-induced accumulation of transcripts associated with the water-water cyclee4
- Direction
- compromisede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e4“However, the heat-induced accumulation of transcripts associated with the water-water cycle was compromised by the loss of 2-CP1/2 functions”
Claim · model-written summary
Transcript levels of the autophagy-related genes ATG5 and ATG7 were higher in plants with impaired 2-CP1/2 functions.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- impaired 2-CP1/2 functionse5
- Gene
- ATG5 and ATG7e5
- Outcome
- transcript levels of autophagy-related genes ATG5 and ATG7e5
- Direction
- highere5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e5“The transcript levels of autophagy-related genes ATG5 and ATG7 were higher in plants with impaired 2-CP1/2 functions, and the formation of autophagosomes increased, together with an accumulation of oxidized and insoluble proteins”
Claim · model-written summary
Impaired 2-CP1/2 function increased the formation of autophagosomes.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- impaired 2-CP1/2 functionse5
- Outcome
- formation of autophagosomese5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e5“The transcript levels of autophagy-related genes ATG5 and ATG7 were higher in plants with impaired 2-CP1/2 functions, and the formation of autophagosomes increased, together with an accumulation of oxidized and insoluble proteins”
Claim · model-written summary
Impaired 2-CP1/2 function was accompanied by an accumulation of oxidized and insoluble proteins in tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- accumulation of oxidized and insoluble proteinse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e5“The transcript levels of autophagy-related genes ATG5 and ATG7 were higher in plants with impaired 2-CP1/2 functions, and the formation of autophagosomes increased, together with an accumulation of oxidized and insoluble proteins”
Claim · model-written summary
Silencing of ATG5 or ATG7 increased the levels of 2-CP transcripts and protein.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Silencing of ATG5 or ATG7e6
- Gene
- ATG5 or ATG7e6
- Outcome
- levels of 2-CP transcripts and proteine6
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e6“Silencing of ATG5 or ATG7 increased the levels of 2-CP transcripts and protein but decreased heat stress tolerance”
Claim · model-written summary
Silencing of ATG5 or ATG7 decreased heat stress tolerance in tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Silencing of ATG5 or ATG7e6
- Outcome
- heat stress tolerancee6
- Direction
- decreasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e6“Silencing of ATG5 or ATG7 increased the levels of 2-CP transcripts and protein but decreased heat stress tolerance”
Claim · model-written summary
The authors conclude that 2-CPs fulfil a pivotal role in heat stress tolerance in tomato, via interactions with ascorbate-dependent pathways and autophagy.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- heat stress tolerancee7
- Qualifier
- via interactions with ascorbate-dependent pathways and autophagye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants”
e7“These results demonstrate that 2-CPs fulfil a pivotal role in heat stress tolerance in tomato, via interactions with ascorbate-dependent pathways and autophagy”
The same questions in other papers
loss of function · organism · Solanum lycopersicum (tomato)Positive and negative results
6 papers in the same direction as this paper · 3 in the opposite direction
- negative
- positive
- positive
6 papers more in this group
See all 10 papers →loss of function · tolerance: abiotic · Solanum lycopersicum (tomato)Positive and negative results
40 papers in the same direction as this paper · 12 in the opposite direction
- negative
- negative
- positive
49 papers more in this group
See all 53 papers →loss of function · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
48 papers in the same direction as this paper · 18 in the opposite direction
- negative
- positive
- positive
63 papers more in this group
See all 67 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.