Abstract
In-vitro organogenesis is the best method to overcome the problem of somaclonal variations and obtain trueto-type date palm plants. In the present investigation, activated charcoal (AC) (1.5 and 3.0 g l−1), polyvinylpyrrolidone (PVP) (1.0 and 2.0 g l−1) and thiourea (250.0 and 500.0 mg l−1) were separately added to the half-strength Murashige and Skoog medium to test the survival, browning of tissue/media and suitability of the growth of explants. Date palm cv. Halawy seedlings shoot tip cultured under dark condition showed that AC (1.5 g l−1) was suitable for explant survival, minimized browning and supported growth for one month than other treatments. Half-strength MS medium supplemented with 1.5 g l−1 AC and different treatment combinations NAA (0.0, 0.1 and 1.0 mg l−1) with either 2-ip or TDZ (1.5 and 3.0 mg l−1) were used for axillary shoot production in dark conditions. Out of 12 treatments, 3.0 mg l−1 2-iP + 0.1 mg l−1 NAA was superior for axillary shoots production within three months. Halfstrength MS medium supplemented with different concentrations of GA3 (0.0, 0.25, 0.5, 1.0 and 2.0 mg l−1) along with 1.0 mg l−1 NAA was used for shoot elongation. These cultures were kept under 16 h light (2000 lux) and 8 h dark for one month. Out of five treatments tested, 0.5 mg l−1GA3 + 1.0 mg l−1 NAA was found optimum for shoot elongation. The results drawn from this study will be highly useful in micropropagation of date palm seedlings compared to off-shoot/sucker by organogenesis.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Activated charcoal at 1.5 g l−1 minimized browning of date palm shoot tips cultured in the dark.
- Taxon
- Date palm
Phoenix dactyliferae0 - Material
- cv. Halawye0
- Organ / stage
- shoot tipe0
- Treatment
- AC (1.5 g l−1)e0
- Outcome
- browninge0
- Direction
- minimizede0
- Comparator
- other treatmentse0
- Setting
- dark conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Date palm cv. Halawy seedlings shoot tip cultured under dark condition showed that AC (1.5 g l−1) was suitable for explant survival, minimized browning and supported growth for one month than other treatments”
Claim · model-written summary
Activated charcoal at 1.5 g l−1 was suitable for explant survival of date palm shoot tips compared with other treatments.
- Taxon
- Date palmPhoenix dactyliferae0
- Material
- cv. Halawye0
- Organ / stage
- shoot tipe0
- Treatment
- AC (1.5 g l−1)e0
- Outcome
- explant survivale0
- Direction
- suitablee0
- Comparator
- other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Date palm cv. Halawy seedlings shoot tip cultured under dark condition showed that AC (1.5 g l−1) was suitable for explant survival, minimized browning and supported growth for one month than other treatments”
Claim · model-written summary
Activated charcoal at 1.5 g l−1 supported growth of date palm shoot tips for one month compared with other treatments.
- Taxon
- Date palmPhoenix dactyliferae0
- Material
- cv. Halawye0
- Organ / stage
- shoot tipe0
- Treatment
- AC (1.5 g l−1)e0
- Outcome
- growthe0
- Direction
- supportede0
- Comparator
- other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Date palm cv. Halawy seedlings shoot tip cultured under dark condition showed that AC (1.5 g l−1) was suitable for explant survival, minimized browning and supported growth for one month than other treatments”
Claim · model-written summary
3.0 mg l−1 2-iP with 0.1 mg l−1 NAA was superior for axillary shoot production within three months.
- Taxon
- Date palmPhoenix dactyliferae0
- Treatment
- 3.0 mg l−1 2-iP + 0.1 mg l−1 NAAe1
- Outcome
- axillary shoots productione1
- Direction
- superiore1
- Comparator
- 12 treatmentse1
- Setting
- dark conditionse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Date palm cv. Halawy seedlings shoot tip cultured under dark condition showed that AC (1.5 g l−1) was suitable for explant survival, minimized browning and supported growth for one month than other treatments”
e1“Out of 12 treatments, 3.0 mg l−1 2-iP + 0.1 mg l−1 NAA was superior for axillary shoots production within three months”
e2“were used for axillary shoot production in dark conditions”
Claim · model-written summary
0.5 mg l−1 GA3 with 1.0 mg l−1 NAA was optimum for shoot elongation of date palm.
- Taxon
- Date palmPhoenix dactyliferae0
- Treatment
- 0.5 mg l−1GA3 + 1.0 mg l−1 NAAe3
- Outcome
- shoot elongatione3
- Direction
- optimume3
- Comparator
- five treatmentse3
- Setting
- 16 h light (2000 lux) and 8 h darke4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Date palm cv. Halawy seedlings shoot tip cultured under dark condition showed that AC (1.5 g l−1) was suitable for explant survival, minimized browning and supported growth for one month than other treatments”
e3“Out of five treatments tested, 0.5 mg l−1GA3 + 1.0 mg l−1 NAA was found optimum for shoot elongation”
e4“These cultures were kept under 16 h light (2000 lux) and 8 h dark for one month”
The same questions in other papers
charcoal · growth · Phoenix dactylifera (date palm)Results differ
14 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
12 papers more in this group
See all 16 papers →1-naphthaleneacetic acid · growth · Phoenix dactylifera (date palm)Positive and negative results
37 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
35 papers more in this group
See all 39 papers →charcoal · disease · Phoenix dactylifera (date palm)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Gibberellic acid · growth · Phoenix dactylifera (date palm)Results agree
9 papers in the same direction as this paper
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →1-naphthaleneacetic acid · yield · Phoenix dactylifera (date palm)Results agree
15 papers in the same direction as this paper
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.