Abstract
A good potential for in vitro regeneration is needed to produce useful plants in genetic improvement programs. Various protocols have been used to improve specifically soybean and cowpea cultivars regeneration. In this study, one cultivar of soybean, Doko, and four cowpea cultivars (Epace 10, Joao Paulo II, Setentao and Pitiuba) served to provide explants for in vitro culture. Zygotic embryos and cotyledons extracted from seeds and segments of hypocotyls taken from germinated seedlings have been cultivated in supplemented environment with different growth regulators (2,4-D, TDZ and BA). MS environment containing BA and 2, 4-D (5 µM) results on a better callus and bud formation on zygotic embryos and cotyledons explants of the cultivar Doko. Bud formation from zygotic explants of this cultivar was higher in average in relation to the four cultivars of cowpea cultured in a supplemented environment with BA (6. 6 µM) and sucrose (3%). These in vitro morphogenetic responses don't allow a further utilization of these materials for genetic improvement.
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Claims 3
Claim · model-written summary
BA and 2,4-D medium improved callus and bud formation in Doko soybean zygotic embryos and cotyledons.
- Taxon
- soybean
Glycine maxt0 - Material
- Dokoe0
- Organ / stage
- zygotic embryos and cotyledonse0
- Treatment
- MS environment containing BA and 2, 4-D (5 µM)e0
- Outcome
- callus and bud formatione0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“MS environment containing BA and 2, 4-D (5 µM) results on a better callus and bud formation on zygotic embryos and cotyledons explants of the cultivar Doko”
Claim · model-written summary
Doko soybean zygotic explants formed more buds on average than four cowpea cultivars in BA and sucrose medium.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- zygotic explantse0
- Treatment
- BA (6. 6 µM) and sucrose (3%)e0
- Outcome
- Bud formatione0
- Direction
- highere0
- Comparator
- four cultivars of cowpeae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Bud formation from zygotic explants of this cultivar was higher in average in relation to the four cultivars of cowpea cultured in a supplemented environment with BA (6. 6 µM) and sucrose (3%)”
Claim · model-written summary
The observed in vitro responses were judged unsuitable for further genetic improvement use.
- Taxon
- cowpea and soybeanGlycine max
Vigna unguiculatat0 - Outcome
- further utilization of these materials for genetic improvemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cowpea and soybean”
e0“These in vitro morphogenetic responses don't allow a further utilization of these materials for genetic improvement”
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