Abstract
Effects of bensulfuron methyl (BSM) on amino acid and protein content were compared in normal and 10-5M BSM tolerant rice cells. Culture in the presence of BSM led to a dramatic decrease in the level of branched-chain amino acids to 25-49% of untreated in normal rice cells over 48hr, while they increased in BSM-tolerant cells. The other amino acids in exposed normal cells increased substantially, but those amino acids in tolerant cells changed relatively little. The inhibition of protein biosynthesis by BSM appeared to cause a decreased level of protein content in normal cells, while almost no effect in tolerant cells. The feedback inhibition by one or more of the end-products, valine, leucine and isoleucine, of acetolactate synthase (ALS) from normal and tolerant cells was also investigated. The activity of extractable enzyme from both cell types was feedback inhibited by the three amino acids Val+Leu, Val, Leu, Ile: 39, 41, 45, 58% of control at 5mM, but the enzyme of tolerant cells had less feedback sensitivity: 49, 55, 57, 80%, respectively. Based on these findings, a high level of branched-chain amino acid biosynthesis by the BSM-insensitively modified ALS and less sensitivity of the enzyme to branched-chain amino acids may provide protection against BSM in the tolerant cells.
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Claims 9
Claim · model-written summary
BSM reduced branched-chain amino acids in normal rice cells to 25-49% of untreated levels.
- Taxon
- rice
Oryza sativae0 - Material
- normal rice cellse0
- Treatment
- BSMe0
- Outcome
- level of branched-chain amino acidse0
- Direction
- decreasee0
- Value
- 25-49%e0
- Qualifier
- over 48hre0
- Comparator
- untreatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culture in the presence of BSM led to a dramatic decrease in the level of branched-chain amino acids to 25-49% of untreated in normal rice cells over 48hr”
Claim · model-written summary
Branched-chain amino acids increased in BSM-tolerant rice cells.
- Taxon
- RiceOryza sativat0
- Material
- BSM-tolerant cellse0
- Treatment
- BSMe0
- Outcome
- branched-chain amino acidse1
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while they increased in BSM-tolerant cells”
e1“branched-chain amino acids to 25-49% of untreated in normal rice cells”
t0“Rice Cells”
Claim · model-written summary
Other amino acids increased substantially in exposed normal cells.
- Taxon
- RiceOryza sativat0
- Material
- normal cellse0
- Treatment
- BSMe1
- Outcome
- other amino acidse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The other amino acids in exposed normal cells increased substantially”
t0“Rice Cells”
e1“Effects of bensulfuron methyl (BSM) on amino acid and protein content were compared in normal and 10-5M BSM tolerant rice cells”
Claim · model-written summary
Other amino acids changed relatively little in tolerant cells.
- Taxon
- riceOryza sativae1
- Material
- tolerant cellse0
- Treatment
- BSMe1
- Outcome
- those amino acidse0
- Direction
- changed relatively littlee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those amino acids in tolerant cells changed relatively little”
e1“Effects of bensulfuron methyl (BSM) on amino acid and protein content were compared in normal and 10-5M BSM tolerant rice cells”
Claim · model-written summary
BSM decreased protein content in normal rice cells.
- Taxon
- RiceOryza sativat0
- Material
- normal cellse0
- Treatment
- BSMe0
- Outcome
- protein contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition of protein biosynthesis by BSM appeared to cause a decreased level of protein content in normal cells”
t0“Rice Cells”
Claim · model-written summary
BSM had almost no effect on protein content in tolerant rice cells.
- Taxon
- RiceOryza sativat0
- Material
- tolerant cellse0
- Treatment
- BSMe1
- Outcome
- protein contente1
- Direction
- almost no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while almost no effect in tolerant cells”
e1“The inhibition of protein biosynthesis by BSM appeared to cause a decreased level of protein content in normal cells”
t0“Rice Cells”
Claim · model-written summary
Branched-chain amino acids feedback inhibited ALS activity in normal rice cells, with four reported activities of 39, 41, 45 and 58% of control.
- Taxon
- RiceOryza sativat0
- Gene
- acetolactate synthase (ALS)e1
- Outcome
- activity of extractable enzymee0
- Direction
- feedback inhibitede0
- Value
- 39, 41, 45, 58% of controle0
- Qualifier
- at 5mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of extractable enzyme from both cell types was feedback inhibited by the three amino acids Val+Leu, Val, Leu, Ile: 39, 41, 45, 58% of control at 5mM”
e1“acetolactate synthase (ALS) from normal and tolerant cells”
t0“Rice Cells”
Claim · model-written summary
ALS in tolerant rice cells had less feedback sensitivity, with activities of 49, 55, 57 and 80%.
- Taxon
- RiceOryza sativat0
- Material
- tolerant cellse0
- Gene
- acetolactate synthase (ALS)e1
- Outcome
- feedback sensitivitye0
- Direction
- lesse0
- Value
- 49, 55, 57, 80%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enzyme of tolerant cells had less feedback sensitivity: 49, 55, 57, 80%, respectively”
e1“acetolactate synthase (ALS) from normal and tolerant cells”
t0“Rice Cells”
Claim · model-written summary
The authors propose modified ALS biosynthesis and reduced feedback sensitivity protect tolerant rice cells against BSM.
- Taxon
- RiceOryza sativat0
- Gene
- ALSe0
- Outcome
- protection against BSMe0
- Qualifier
- less sensitivity of the enzyme to branched-chain amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a high level of branched-chain amino acid biosynthesis by the BSM-insensitively modified ALS and less sensitivity of the enzyme to branched-chain amino acids may provide protection against BSM in the tolerant cells”
t0“Rice Cells”
The same questions in other papers
bensulfuron-methyl · molecular: metabolites · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.