Abstract
Cultured stem nodal sections from a greenhouse-grown miniature rose (Rosa hybrida L. 'Petite Folie') proliferated shoots after 5 weeks on Murashige and Skoog (MS) medium with the following ratios of growth regulators: 0, 0.1, 0.3, 1, 10, or 30 mg/liter 6-benzyladenine (BA) to 0, 0.1, 0.3, or 1 mg/liter napthaleneacetic acid (NAA). Use of 3 mg/liter BA alone or 3 mg/ liter BA plus 0. 1 mg/liter NAA resulted in the greatest shoot multiplication. Comparisons were made for shoot multiplication among MS, Schenk and Hildebrandt (SH), and Woody Plant Medium (WPM) media supplemented with 3 mg/liter BA and 0.05 mg/liter NAA. MS medium was most effective. No further increase in multiplication was obtained by extending the length of the culture period beyond five weeks. The microshoots were rooted in vitro after eight days on 0.5 strength MS medium supplemented with 0.5 mg/liter NAA or 0.1 mg/liter indolebutyric acid (IBA). Miniature roses are traditionally propagated by stem cuttings. However, hybrid tea, climber, and floribunda roses are usually propagated by budgrafting of the desired cultivar onto a different rootstock. In vitro clonal propagation of roses is of great commercial value. Using this technique, many pathogen-free rose plants can be produced. Tissue culture propagation of Rosa hybrida cultivars and Rosa species from isolated shoot tips has been successful (Davies, 1980; Elliott, 1970; Hasegawa, 1980; Jacobs et al., 1969, 1970a, 1970b; Khosh-Khui and Sink, 1982a, 1982b). The use of tissue culture techniques should minimize the time necessary for the introduction of new cultivars into the commercial market, and thus increase the availability of rose plants with improved horticultural characteristics. The use of in vitro culture for the propagation of cut flower and outdoor rose types requires that these roses perform at least as well on their own roots as on grafted rootstocks (Buck, 1951; Holley, 1969). This paper reports the effects of different media supplemented with selected levels of auxin and cytokinin on shoot multiplication. It also reports the effect of auxin on rooting cultured 'Petite Folie' shoots. This content downloaded from 207.46.13.11 on Wed, 10 Aug 2016 06:33:03 UTC All use subject to http://about.jstor.org/terms VOLUME 94, NUMBERS 3-4 117 Table 1. The basal media and supplements used in the in vitro propagation of 'Petite Folie' miniature rose.
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Claims 4
Claim · model-written summary
Three mg/liter BA alone or with 0.1 mg/liter NAA gave the greatest shoot multiplication
- Taxon
- Rosa hybrida L.
Rosa hybrid cultivare0 - Material
- Petite Foliee0
- Treatment
- 3 mg/liter BA alone or 3 mg/ liter BA plus 0. 1 mg/liter NAAe2
- Outcome
- shoot multiplicatione2
- Direction
- greateste2
- Comparator
- 0, 0.1, 0.3, 1, 10, or 30 mg/liter 6-benzyladenine (BA)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultured stem nodal sections from a greenhouse-grown miniature rose (Rosa hybrida L. 'Petite Folie')”
e1“0, 0.1, 0.3, 1, 10, or 30 mg/liter 6-benzyladenine (BA)”
e2“Use of 3 mg/liter BA alone or 3 mg/ liter BA plus 0. 1 mg/liter NAA resulted in the greatest shoot multiplication”
Claim · model-written summary
MS medium was the most effective of the three media for shoot multiplication
- Taxon
- Rosa hybrida L.Rosa hybrid cultivare0
- Treatment
- MS mediume3
- Outcome
- shoot multiplicatione3
- Direction
- most effectivee3
- Comparator
- Schenk and Hildebrandt (SH), and Woody Plant Medium (WPM)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultured stem nodal sections from a greenhouse-grown miniature rose (Rosa hybrida L. 'Petite Folie')”
e3“Comparisons were made for shoot multiplication among MS, Schenk and Hildebrandt (SH), and Woody Plant Medium (WPM) media supplemented with 3 mg/liter BA and 0.05 mg/liter NAA. MS medium was most effective”
Claim · model-written summary
Microshoots rooted in vitro after eight days on 0.5 strength MS medium with NAA or IBA
- Taxon
- Rosa hybrida L.Rosa hybrid cultivare0
- Treatment
- 0.5 strength MS medium supplemented with 0.5 mg/liter NAA or 0.1 mg/liter indolebutyric acid (IBA)e5
- Outcome
- rooted in vitroe5
- Setting
- in vitroe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultured stem nodal sections from a greenhouse-grown miniature rose (Rosa hybrida L. 'Petite Folie')”
e5“The microshoots were rooted in vitro after eight days on 0.5 strength MS medium supplemented with 0.5 mg/liter NAA or 0.1 mg/liter indolebutyric acid (IBA)”
Claim · model-written summary
Extending the culture period beyond five weeks gave no further increase in shoot multiplication
- Taxon
- Rosa hybrida L.Rosa hybrid cultivare0
- Treatment
- extending the length of the culture period beyond five weekse4
- Outcome
- multiplicatione4
- Direction
- No further increasee4
- Comparator
- five weekse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultured stem nodal sections from a greenhouse-grown miniature rose (Rosa hybrida L. 'Petite Folie')”
e4“No further increase in multiplication was obtained by extending the length of the culture period beyond five weeks”
The same questions in other papers
N-benzyladenine · pest · Rosa hybrid cultivarResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
in vitro · pest · Rosa hybrid cultivarResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.