Abstract
In vitro culture techniques may be valuable tools to increase breeding efficiency, introduce genotypes with interesting ornamental traits and shorten the time needed to obtain new cultivars. Hips resulting from a specific tetraploid Rosa hybrida cross were weekly harvested from 21 to 35 days after pollination (DAP). The obtained in vitro seedlings were multiplied using two media: M1) MS and M2) MS added with BAP (1 mg L -1 ) and GA3 (0.5 mg L -1 ). The highest multiplication rate was obtained starting from embryos collected at 35 DAP on MS medium added with hormones. The shoot sprouting and the abnormal rate were generally not affected by the presence of hormones in the medium. For rooting, two media were also tested: R1) MS and R2) MS supplemented with IBA (0.1 mg L -1 ). The highest percentage of rooted explants was obtained starting from embryos collected at 28 DAP on R1 medium. The same conditions induced also the highest number of roots. The addition of IBA to rooting medium induced the development of longer roots in explants obtained by embryos collected at 35 DAP. The rooted plantlets were successfully acclimatized to ex vitro conditions. At flowering, the morphological traits of eight clone groups were compared with those of the seedlings obtained under common practices. Ex vitro plantlets showed more variability in flower colours, presence/absence of prickles, and number of petals per flower.
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Claims 7
Claim · model-written summary
Embryos collected at 35 DAP on hormone-supplemented MS medium gave the highest multiplication rate.
- Taxon
- Rosa hybrida
Rosa hybrid cultivart0 - Organ / stage
- embryose0
- Treatment
- MS medium added with hormonese0
- Outcome
- multiplication ratee0
- Direction
- higheste0
- Qualifier
- 35 DAPe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rosa hybrida”
e0“The highest multiplication rate was obtained starting from embryos collected at 35 DAP on MS medium added with hormones.”
Claim · model-written summary
Hormones generally did not affect shoot sprouting or abnormality rate.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- presence of hormones in the mediume0
- Outcome
- shoot sprouting and the abnormal ratee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rosa hybrida”
e0“The shoot sprouting and the abnormal rate were generally not affected by the presence of hormones in the medium.”
Claim · model-written summary
Embryos collected at 28 DAP on R1 medium gave the highest percentage of rooted explants.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Organ / stage
- embryose0
- Treatment
- R1 mediume0
- Outcome
- percentage of rooted explantse0
- Direction
- higheste0
- Qualifier
- 28 DAPe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rosa hybrida”
e0“The highest percentage of rooted explants was obtained starting from embryos collected at 28 DAP on R1 medium.”
Claim · model-written summary
The same 28 DAP and R1 conditions gave the highest root number.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- R1 mediume0
- Outcome
- number of rootse1
- Direction
- higheste1
- Qualifier
- 28 DAPe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rosa hybrida”
e0“The highest percentage of rooted explants was obtained starting from embryos collected at 28 DAP on R1 medium.”
e1“The same conditions induced also the highest number of roots.”
Claim · model-written summary
IBA in rooting medium lengthened roots from embryos collected at 35 DAP.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Treatment
- addition of IBA to rooting mediume0
- Outcome
- rootse0
- Direction
- longere0
- Qualifier
- 35 DAPe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rosa hybrida”
e0“The addition of IBA to rooting medium induced the development of longer roots in explants obtained by embryos collected at 35 DAP.”
Claim · model-written summary
Rooted plantlets were successfully acclimatized ex vitro.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Organ / stage
- rooted plantletse0
- Outcome
- successfully acclimatizede0
- Setting
- ex vitro conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rosa hybrida”
e0“The rooted plantlets were successfully acclimatized to ex vitro conditions.”
Claim · model-written summary
Ex vitro plantlets showed greater variation in flower color, prickles, and petal number.
- Taxon
- Rosa hybridaRosa hybrid cultivart0
- Organ / stage
- Ex vitro plantletse0
- Outcome
- variability in flower colours, presence/absence of prickles, and number of petals per flowere0
- Direction
- moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rosa hybrida”
e0“Ex vitro plantlets showed more variability in flower colours, presence/absence of prickles, and number of petals per flower.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.