Abstract
The present research was carried out to study the effect of BAP at 0.25, 0.50, 1.00 and 2.00 mg/l concentrations added to the multiplication medium and IAA at 0.1, 0.3, 1.0 and 3.0 mg/l concentrations applied in rooting medium on the in vitro micropropagation and acclimatization under greenhouse conditions of Rosa hybrida L. plants. The rose cultivars used in this research were namely Athena, Diana and Red Success. To evaluate the adaptation degree of different rose cultivars to culture medium composition, the shoot tips (15 – 20 mm length) were surface sterilized and cultured on the modified Quoirin & Lepoivre (1977) medium used as proliferation medium supplemented with casein hydrolysate (1.0 g/l), sucrose (30.0 g/l), Difco agar (8.0 g/l), gibberellic acid (GA3) (1.0 mg/l), BAP (1.0 mg/l) and IAA (0.3 mg/l). The proliferated shoots were excised and cultured on multiplication and rooting media. The obtained results indicated that Red success rose cv. were the best to produce the highest rate of shoot proliferation, shoot multiplication, rhizogenesis values and survival percentage followed by Diana rose cv., while Athena rose was the lowest in this respect. The obtained results showed that the best shoot multiplication was achieved in the culture medium containing 6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l) which produced the highest survival percentage, shoot initiation occurred within a few days, the highest percentage of explants which formed shoots, the highest number of multiplied shoots and the tallest multiplied shoots per explant. The obtained results also indicated that the BAP concentration of the shoot multiplication medium affected rooting and survival percentages in the next stage of culture. Shoot tips formed on media which containing 0.25 and 0.50 mg/l BAP rooted and acclimatized better than shoot tips formed on media containing 1.0 and 2.0 mg/l BAP. Moreover, rooting of multiplied shoots was achieved on the same medium used in multiplication stage except for the macroelements of Quoirin & Lepoivre (1977) medium used at half strength and sucrose at 20 g/l and IAA (0.3 mg/l) which produced the highest rooting percentage, the highest number of roots per explant and the tallest roots per explant. The obtained results also revealed that fewer amount of IAA (0.1 mg/l) in the rooting media significantly improved plantlets acclimatization, and subsequently transferred to a mixture of peatmoss and perlite (3:1 v/v) to greenhouse. The rooted plantlets survived Successfully and grew into normal plants.
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Claims 13
Claim · model-written summary
Red Success rose gave the highest rate of shoot proliferation and shoot multiplication among the tested cultivars.
- Taxon
- Rosa hybrida L.
Rosa hybrid cultivart0 - Material
- Red success rose cv.e0
- Outcome
- shoot proliferation, shoot multiplicatione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red success rose cv. were the best to produce the highest rate of shoot proliferation, shoot multiplication”
t0“Rosa hybrida L.”
Claim · model-written summary
Red Success rose gave the highest rhizogenesis values and survival percentage among the tested cultivars.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Material
- Red success rose cv.e0
- Outcome
- rhizogenesis values and survival percentagee0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red success rose cv. were the best to produce the highest rate of shoot proliferation, shoot multiplication, rhizogenesis values and survival percentage”
t0“Rosa hybrida L.”
Claim · model-written summary
Diana rose followed Red Success in shoot proliferation, shoot multiplication, rhizogenesis values and survival percentage.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Material
- Diana rose cv.e0
- Outcome
- shoot proliferation, shoot multiplication, rhizogenesis values and survival percentagee0
- Direction
- followed bye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot proliferation, shoot multiplication, rhizogenesis values and survival percentage followed by Diana rose cv.”
t0“Rosa hybrida L.”
Claim · model-written summary
Athena rose was the lowest among the cultivars in shoot proliferation, shoot multiplication, rhizogenesis values and survival percentage.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Material
- Athena rosee0
- Outcome
- lowest in this respecte0
- Direction
- loweste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Athena rose was the lowest in this respect”
t0“Rosa hybrida L.”
Claim · model-written summary
The best shoot multiplication was achieved in medium containing BAP at 1.0 mg/l and IAA at 0.5 mg/l.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- 6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l)e0
- Outcome
- shoot multiplicatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the best shoot multiplication was achieved in the culture medium containing 6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l)”
t0“Rosa hybrida L.”
Claim · model-written summary
Medium with BAP at 1.0 mg/l and IAA at 0.5 mg/l produced the highest survival percentage.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- 6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l)e0
- Outcome
- survival percentagee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l) which produced the highest survival percentage”
t0“Rosa hybrida L.”
Claim · model-written summary
Medium with BAP at 1.0 mg/l and IAA at 0.5 mg/l produced the highest percentage of explants forming shoots, the highest number of multiplied shoots and the tallest multiplied shoots per explant.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- 6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l)e0
- Outcome
- percentage of explants which formed shoots, the highest number of multiplied shoots and the tallest multiplied shoots per explante0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l) which produced the highest survival percentage, shoot initiation occurred within a few days, the highest percentage of explants which formed shoots, the highest number of multiplied shoots and the tallest multiplied shoots per explant”
t0“Rosa hybrida L.”
Claim · model-written summary
On medium with BAP at 1.0 mg/l and IAA at 0.5 mg/l, shoot initiation occurred within a few days.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- 6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l)e0
- Outcome
- shoot initiatione0
- Qualifier
- within a few dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6-benzylaminopurine (BAP) (1.0 mg/l) and indole-3-acetic acid (IAA) (0.5 mg/l) which produced the highest survival percentage, shoot initiation occurred within a few days”
t0“Rosa hybrida L.”
Claim · model-written summary
The BAP concentration of the shoot multiplication medium affected rooting and survival percentages in the next culture stage.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- BAP concentration of the shoot multiplication mediume0
- Outcome
- rooting and survival percentagese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the BAP concentration of the shoot multiplication medium affected rooting and survival percentages in the next stage of culture”
t0“Rosa hybrida L.”
Claim · model-written summary
Shoot tips formed on media with 0.25 and 0.50 mg/l BAP rooted and acclimatized better than those formed on media with 1.0 and 2.0 mg/l BAP.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- media which containing 0.25 and 0.50 mg/l BAPe0
- Outcome
- rooted and acclimatizede0
- Direction
- bettere0
- Comparator
- media containing 1.0 and 2.0 mg/l BAPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot tips formed on media which containing 0.25 and 0.50 mg/l BAP rooted and acclimatized better than shoot tips formed on media containing 1.0 and 2.0 mg/l BAP”
t0“Rosa hybrida L.”
Claim · model-written summary
Rooting medium with half-strength macroelements, sucrose at 20 g/l and IAA at 0.3 mg/l produced the highest rooting percentage, number of roots per explant and tallest roots per explant.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- macroelements of Quoirin & Lepoivre (1977) medium used at half strength and sucrose at 20 g/l and IAA (0.3 mg/l)e0
- Outcome
- rooting percentage, the highest number of roots per explant and the tallest roots per explante0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the macroelements of Quoirin & Lepoivre (1977) medium used at half strength and sucrose at 20 g/l and IAA (0.3 mg/l) which produced the highest rooting percentage, the highest number of roots per explant and the tallest roots per explant”
t0“Rosa hybrida L.”
Claim · model-written summary
A lower amount of IAA (0.1 mg/l) in the rooting medium significantly improved plantlet acclimatization.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Treatment
- IAA (0.1 mg/l) in the rooting mediae0
- Outcome
- plantlets acclimatizatione0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fewer amount of IAA (0.1 mg/l) in the rooting media significantly improved plantlets acclimatization”
t0“Rosa hybrida L.”
Claim · model-written summary
The rooted plantlets survived successfully and grew into normal plants.
- Taxon
- Rosa hybrida L.Rosa hybrid cultivart0
- Outcome
- survived Successfully and grew into normal plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rooted plantlets survived Successfully and grew into normal plants”
t0“Rosa hybrida L.”
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