Abstract
The activity of peroxidase (PRX) isozyme, lipid peroxidation (Malondialdehyde, MDA content) and cell membrane injury were studied during low temperature treatment for different periods in strawberry (Fragaria x ananassa cv. Camarosa) leaf tissues. Seedlings were grown for six weeks (plants had 4-5 leaves) in a greenhouse then the plants were transferred to a climate chamber with constant 5 o C, 60% relative humidity, 14/10 h (light/dark) photoperiod regime and 4 LS light intensity for 1, 4, 7 or 10 days to impose a low temperature stress. In general, low temperature application during 10 days caused a linear increase in MDA content. Native polyacrylamide gel electrophoresis (PAGE) of both acidic and basic peroxidase (PRX) isozymes yielded a single sharp protein band with Rf=0.23 and Rf=0.17, respectively. In addition data indicated a strong relationship between band intensities and the duration of the low temperature treatment. However, the considerable increase of PRX activities could not stop the deleterious effects of low temperature, but reduced severity of stress, thus showing a reduction in the percentage of injury on the 7 th day which is correlated with cold-acclimation of strawberry leaf tissues under low temperature.
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Claims 6
Claim · model-written summary
Ten days of low temperature caused a linear rise in MDA content.
- Taxon
- strawberry
Fragaria ×ananassat0 - Treatment
- low temperature application during 10 dayse0
- Outcome
- MDA contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“In general, low temperature application during 10 days caused a linear increase in MDA content.”
t0“Peroxidase Activity and Lipid Peroxidation in Strawberry (Fragaria X ananassa) Plants Under Low Temperature”
Claim · model-written summary
Acidic PRX electrophoresis yielded one band at Rf=0.23.
- Taxon
- strawberry
Fragaria ×ananassat0 - Gene
- acidic and basic peroxidase (PRX) isozymese0
- Outcome
- single sharp protein bande0
- Value
- Rf=0.23e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Native polyacrylamide gel electrophoresis (PAGE) of both acidic and basic peroxidase (PRX) isozymes yielded a single sharp protein band with Rf=0.23 and Rf=0.17, respectively.”
t0“Peroxidase Activity and Lipid Peroxidation in Strawberry (Fragaria X ananassa) Plants Under Low Temperature”
Claim · model-written summary
Basic PRX electrophoresis yielded one band at Rf=0.17.
- Taxon
- strawberry
Fragaria ×ananassat0 - Gene
- acidic and basic peroxidase (PRX) isozymese0
- Outcome
- single sharp protein bande0
- Value
- Rf=0.17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Native polyacrylamide gel electrophoresis (PAGE) of both acidic and basic peroxidase (PRX) isozymes yielded a single sharp protein band with Rf=0.23 and Rf=0.17, respectively.”
t0“Peroxidase Activity and Lipid Peroxidation in Strawberry (Fragaria X ananassa) Plants Under Low Temperature”
Claim · model-written summary
PRX band intensity was strongly related to low temperature duration.
- Taxon
- strawberry
Fragaria ×ananassat0 - Treatment
- low temperature treatmente0
- Outcome
- band intensitiese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“In addition data indicated a strong relationship between band intensities and the duration of the low temperature treatment.”
t0“Peroxidase Activity and Lipid Peroxidation in Strawberry (Fragaria X ananassa) Plants Under Low Temperature”
Claim · model-written summary
Increased PRX activity reduced stress severity but did not stop cold injury.
- Taxon
- strawberry
Fragaria ×ananassat0 - Treatment
- low temperaturee0
- Gene
- PRXe0
- Outcome
- severity of stresse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“However, the considerable increase of PRX activities could not stop the deleterious effects of low temperature, but reduced severity of stress”
t0“Peroxidase Activity and Lipid Peroxidation in Strawberry (Fragaria X ananassa) Plants Under Low Temperature”
Claim · model-written summary
Injury percentage fell on day seven in association with cold acclimation.
- Taxon
- strawberry
Fragaria ×ananassae0 - Organ / stage
- leaf tissuese0
- Outcome
- percentage of injurye0
- Direction
- reductione0
- Qualifier
- 7 th daye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“thus showing a reduction in the percentage of injury on the 7 th day which is correlated with cold-acclimation of strawberry leaf tissues under low temperature.”
The same questions in other papers
cold · physiology: oxidative · Fragaria x ananassa (strawberry)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.