Paper · 1971 · Japanese Journal of Crop Science

Studies on the Growth of Tobacco Roots : VIII. The effects of medium condition on the nicotine synthesis in root tips

Teruyoshi NAGAO; Seiko IMAIZUMI

8
claims
17
verbatim quotes
0
citations
1
research areas
✓
Open access

Abstract

In order to determine the effect on the nicotine synthesis, excised root tips were incubated in the media varying in concentration of nitrogen, sugar and gibberellin. The results obtained were as follows. 1. The contents of total nitrogen and total sugar in the big root tips of several varieties were much higher than those in the fine roots, while comparison of nicotine content and the ratio of nicotine-nitrogen to total-nitrogen between big roots and fine ones showed that the values in the former were lower than in the latter. 2. The amount per milligram of synthesized nicotine in the root tips was highest in the roots grown in rooting media with low nitrogen level and those grown in the elongation media with high leyel of nitrogen. 3. The sugar level in the medium seemed to give more effect than nitrogen level. Considerable amount of nicotine was decomposed in the medium of 0.5 per cent sugar level and optimum concentration of sugar for nicotine synthesis was found to be around 2 per cent. 4. With increasing gibberellin concentration from 0 ppm to 1000 ppm, the activities of nicotine synthesis decreased.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Big tobacco root tips contained more total nitrogen and total sugar than fine roots.

Organ / stage
big root tipse0
Outcome
total nitrogen and total sugare0
Direction
much highere0
Comparator
fine rootse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“The contents of total nitrogen and total sugar in the big root tips of several varieties were much higher than those in the fine roots”

✓ located verbatim in the source · abstract, characters 208–342 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Big tobacco roots had lower nicotine content and nicotine-nitrogen to total-nitrogen ratio than fine roots.

Organ / stage
big rootse0
Outcome
nicotine content and the ratio of nicotine-nitrogen to total-nitrogene0
Direction
lowere0
Comparator
fine onese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“comparison of nicotine content and the ratio of nicotine-nitrogen to total-nitrogen between big roots and fine ones showed that the values in the former were lower than in the latter”

✓ located verbatim in the source · abstract, characters 350–532 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nicotine synthesized per milligram was highest with low nitrogen in rooting media.

Treatment
rooting media with low nitrogen levele0
Outcome
amount per milligram of synthesized nicotinee0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“The amount per milligram of synthesized nicotine in the root tips was highest in the roots grown in rooting media with low nitrogen level”

✓ located verbatim in the source · abstract, characters 537–674 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nicotine synthesized per milligram was highest with high nitrogen in elongation media.

Treatment
elongation media with high leyel of nitrogene0
Outcome
nicotinee1
Direction
higheste1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“those grown in the elongation media with high leyel of nitrogen”

✓ located verbatim in the source · abstract, characters 679–742 · show in text ↑
e1

“The amount per milligram of synthesized nicotine in the root tips was highest”

✓ located verbatim in the source · abstract, characters 537–614 · show in text ↑
Associationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Medium sugar level appeared to affect nicotine synthesis more than nitrogen level.

Treatment
sugar level in the mediume0
Outcome
more effecte0
Comparator
nitrogen levele0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“The sugar level in the medium seemed to give more effect than nitrogen level”

✓ located verbatim in the source · abstract, characters 747–823 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Considerable nicotine decomposed at 0.5 per cent medium sugar.

Treatment
0.5 per cent sugar levele0
Outcome
nicotine was decomposede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“Considerable amount of nicotine was decomposed in the medium of 0.5 per cent sugar level”

✓ located verbatim in the source · abstract, characters 825–913 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Around 2 per cent sugar was optimal for nicotine synthesis.

Treatment
sugare0
Outcome
nicotine synthesise0
Direction
optimume0
Value
2 per cente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“optimum concentration of sugar for nicotine synthesis was found to be around 2 per cent”

✓ located verbatim in the source · abstract, characters 918–1005 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Increasing gibberellin from 0 ppm to 1000 ppm decreased nicotine synthesis activity.

Treatment
gibberellin concentration from 0 ppm to 1000 ppme0
Outcome
activities of nicotine synthesise0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

t0

“Tobacco Roots”

✓ located verbatim in the source · title, characters 25–38 · show in text ↑
e0

“With increasing gibberellin concentration from 0 ppm to 1000 ppm, the activities of nicotine synthesis decreased”

✓ located verbatim in the source · abstract, characters 1010–1122 · show in text ↑

class · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results

14 papers: 9 positive, 5 negative or no effect

4 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

10 papers more in this group

See all 14 papers →

in vitro · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results

16 papers: 10 positive, 6 negative or no effect

9 papers in the same direction as this paper · 6 in the opposite direction

  1. negative
  2. negative
  3. no effect

12 papers more in this group

See all 16 papers →

nitrogen · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results

45 papers: 35 positive, 10 negative or no effect

34 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. negative

41 papers more in this group

See all 45 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.