Abstract
In order to determine the effect on the nicotine synthesis, excised root tips were incubated in the media varying in concentration of nitrogen, sugar and gibberellin. The results obtained were as follows. 1. The contents of total nitrogen and total sugar in the big root tips of several varieties were much higher than those in the fine roots, while comparison of nicotine content and the ratio of nicotine-nitrogen to total-nitrogen between big roots and fine ones showed that the values in the former were lower than in the latter. 2. The amount per milligram of synthesized nicotine in the root tips was highest in the roots grown in rooting media with low nitrogen level and those grown in the elongation media with high leyel of nitrogen. 3. The sugar level in the medium seemed to give more effect than nitrogen level. Considerable amount of nicotine was decomposed in the medium of 0.5 per cent sugar level and optimum concentration of sugar for nicotine synthesis was found to be around 2 per cent. 4. With increasing gibberellin concentration from 0 ppm to 1000 ppm, the activities of nicotine synthesis decreased.
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Claims 8
Claim · model-written summary
Big tobacco root tips contained more total nitrogen and total sugar than fine roots.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Organ / stage
- big root tipse0
- Outcome
- total nitrogen and total sugare0
- Direction
- much highere0
- Comparator
- fine rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“The contents of total nitrogen and total sugar in the big root tips of several varieties were much higher than those in the fine roots”
Claim · model-written summary
Big tobacco roots had lower nicotine content and nicotine-nitrogen to total-nitrogen ratio than fine roots.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Organ / stage
- big rootse0
- Outcome
- nicotine content and the ratio of nicotine-nitrogen to total-nitrogene0
- Direction
- lowere0
- Comparator
- fine onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“comparison of nicotine content and the ratio of nicotine-nitrogen to total-nitrogen between big roots and fine ones showed that the values in the former were lower than in the latter”
Claim · model-written summary
Nicotine synthesized per milligram was highest with low nitrogen in rooting media.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Treatment
- rooting media with low nitrogen levele0
- Outcome
- amount per milligram of synthesized nicotinee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“The amount per milligram of synthesized nicotine in the root tips was highest in the roots grown in rooting media with low nitrogen level”
Claim · model-written summary
Nicotine synthesized per milligram was highest with high nitrogen in elongation media.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Treatment
- elongation media with high leyel of nitrogene0
- Outcome
- nicotinee1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“those grown in the elongation media with high leyel of nitrogen”
e1“The amount per milligram of synthesized nicotine in the root tips was highest”
Claim · model-written summary
Medium sugar level appeared to affect nicotine synthesis more than nitrogen level.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Treatment
- sugar level in the mediume0
- Outcome
- more effecte0
- Comparator
- nitrogen levele0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“The sugar level in the medium seemed to give more effect than nitrogen level”
Claim · model-written summary
Considerable nicotine decomposed at 0.5 per cent medium sugar.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Treatment
- 0.5 per cent sugar levele0
- Outcome
- nicotine was decomposede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“Considerable amount of nicotine was decomposed in the medium of 0.5 per cent sugar level”
Claim · model-written summary
Around 2 per cent sugar was optimal for nicotine synthesis.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Treatment
- sugare0
- Outcome
- nicotine synthesise0
- Direction
- optimume0
- Value
- 2 per cente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“optimum concentration of sugar for nicotine synthesis was found to be around 2 per cent”
Claim · model-written summary
Increasing gibberellin from 0 ppm to 1000 ppm decreased nicotine synthesis activity.
- Taxon
- Tobacco
Nicotiana tabacumt0
- Treatment
- gibberellin concentration from 0 ppm to 1000 ppme0
- Outcome
- activities of nicotine synthesise0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco Roots”
e0“With increasing gibberellin concentration from 0 ppm to 1000 ppm, the activities of nicotine synthesis decreased”
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