Abstract
The effect of subculture and temperature on shoot differentiation of rice callus was examined. The callus tissues were cultured in the medium containing kinetin. Starch granules and other cell constituents of the callus were observed in their green regions. While rooting capacity in Te-Tep remained in long term callus cultures over 15th passage, any shooting was not obtained in Aichi asahi cultures over 9th passage. Variation in temperature condition was effective in callus growth, but not in shoot differentiation. Starch granules were stained purple or reddish purple colour with Lugol's solution. In addition, large granules of dark purple colour were observed in green regions of callus tissues in kinetin medium. Disappearance of the starch granules were observed in the tissues in gibberellin medium after 9 days culture. A technique was investigated for epon-sections of rice callus tissues. Some staining methods of azure II·methylene blue, fuchsine-methylene blue, hematoxylin-safranin and toluidine blue were used. Nuclei, nucleoli, osmiophilic granules and cell walls were observed in the green region. Especially, morphological characteristics were revealed in chloroplasts, starch granules and amyloplasts.
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Claims 9
Claim · model-written summary
Te-Tep rice callus retained rooting capacity beyond the fifteenth passage.
- Taxon
- Rice
Oryza sativat0 - Material
- Te-Tepe0
- Organ / stage
- calluse0
- Outcome
- rooting capacitye0
- Direction
- remainede0
- Value
- 15th passagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooting capacity in Te-Tep remained in long term callus cultures over 15th passage”
t0“Rice Callus Tissues”
Claim · model-written summary
Aichi asahi callus did not produce shoots beyond the ninth passage.
- Taxon
- RiceOryza sativat0
- Material
- Aichi asahie0
- Outcome
- shootinge0
- Direction
- not obtainede0
- Value
- 9th passagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“any shooting was not obtained in Aichi asahi cultures over 9th passage”
t0“Rice Callus Tissues”
Claim · model-written summary
Temperature variation affected callus growth.
- Taxon
- RiceOryza sativat0
- Treatment
- Variation in temperature conditione0
- Outcome
- callus growthe0
- Direction
- effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Variation in temperature condition was effective in callus growth, but not in shoot differentiation.”
t0“Rice Callus Tissues”
Claim · model-written summary
Temperature variation did not affect shoot differentiation.
- Taxon
- RiceOryza sativat0
- Treatment
- Variation in temperature conditione0
- Outcome
- shoot differentiatione0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Variation in temperature condition was effective in callus growth, but not in shoot differentiation.”
t0“Rice Callus Tissues”
Claim · model-written summary
Lugol staining made rice callus starch granules purple or reddish purple.
- Taxon
- RiceOryza sativat0
- Organ / stage
- Starch granulese0
- Treatment
- Lugol's solutione0
- Outcome
- purple or reddish purple coloure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Starch granules were stained purple or reddish purple colour with Lugol's solution.”
t0“Rice Callus Tissues”
Claim · model-written summary
Large dark purple granules appeared in green regions of rice callus on kinetin medium.
- Taxon
- RiceOryza sativat0
- Organ / stage
- callus tissuese0
- Treatment
- kinetin mediume0
- Outcome
- large granules of dark purple coloure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“large granules of dark purple colour were observed in green regions of callus tissues in kinetin medium”
t0“Rice Callus Tissues”
Claim · model-written summary
Starch granules disappeared from rice callus after nine days in gibberellin medium.
- Taxon
- RiceOryza sativat0
- Organ / stage
- tissuese0
- Treatment
- gibberellin mediume0
- Outcome
- starch granulese0
- Direction
- Disappearancee0
- Value
- 9 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disappearance of the starch granules were observed in the tissues in gibberellin medium after 9 days culture.”
t0“Rice Callus Tissues”
Claim · model-written summary
Nuclei, nucleoli, osmiophilic granules and cell walls were observed in green rice callus regions.
- Taxon
- RiceOryza sativat0
- Organ / stage
- green regione0
- Outcome
- Nuclei, nucleoli, osmiophilic granules and cell wallse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclei, nucleoli, osmiophilic granules and cell walls were observed in the green region.”
t0“Rice Callus Tissues”
Claim · model-written summary
Morphological features were revealed in chloroplasts, starch granules and amyloplasts.
- Taxon
- RiceOryza sativat0
- Organ / stage
- chloroplasts, starch granules and amyloplastse0
- Outcome
- morphological characteristicse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“morphological characteristics were revealed in chloroplasts, starch granules and amyloplasts.”
t0“Rice Callus Tissues”
The same questions in other papers
in vitro · quality: sugar starch · Oryza sativa (rice)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
class · quality: sugar starch · Oryza sativa (rice)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.