Abstract
Plant often responds to fungal pathogens by expressing a group of proteins known as pathogenesis-related proteins (PRs). The expression of PR is mediated through pathogen-induced signal-transduction pathways that are fine-tuned by phytohormones such as methyl jasmonate (MeJA). Here, we report functional characterization of an Ocimum basilicum PR5 family member (ObTLP1) that was identified from a MeJA-responsive expression sequence tag collection. ObTLP1 encodes a 226 amino acid polypeptide that showed sequence and structural similarities with a sweet-tasting protein thaumatin of Thaumatococcus danielli and also with a stress-responsive protein osmotin of Nicotiana tabacum. The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition, and responsive to biotic and abiotic stresses, and multiple phytohormone elicitations. Bacterially-expressed recombinant ObTLP1 inhibited mycelial growth of the phytopathogenic fungi, Scleretonia sclerotiorum and Botrytis cinerea; thereby, suggesting its antifungal activity. Ectopic expression of ObTLP1 in Arabidopsis led to enhanced tolerance to S. sclerotiorum and B. cinerea infections, and also to dehydration and salt stress. Moreover, induced expression of the defense marker genes suggested up-regulation of the defense-response pathways in ObTLP1-expressing Arabidopsis upon fungal challenge. Thus, ObTLP1 might be useful for providing tolerance to the fungal pathogens and abiotic stresses in crops.
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Claims 12
Claim · model-written summary
ObTLP1 encodes a 226 amino acid polypeptide similar to thaumatin and osmotin.
- Taxon
- Ocimum basilicum
Ocimum basilicume1 - Gene
- ObTLP1e0
- Outcome
- 226 amino acid polypeptidee0
- Value
- 226 amino acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ObTLP1 encodes a 226 amino acid polypeptide that showed sequence and structural similarities with a sweet-tasting protein thaumatin of Thaumatococcus danielli and also with a stress-responsive protein osmotin of Nicotiana tabacum.”
e1“Ocimum basilicum PR5 family member (ObTLP1)”
Claim · model-written summary
ObTLP1 expression was organ preferential in unstressed O. basilicum.
- Taxon
- O. basilicumOcimum basilicume0
- Gene
- ObTLP1e0
- Outcome
- expression of ObTLP1e0
- Qualifier
- unstressed conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition”
Claim · model-written summary
ObTLP1 expression responded to biotic stress in O. basilicum.
- Taxon
- O. basilicumOcimum basilicume0
- Treatment
- biotice0
- Gene
- ObTLP1e0
- Outcome
- expression of ObTLP1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition, and responsive to biotic and abiotic stresses, and multiple phytohormone elicitations.”
Claim · model-written summary
ObTLP1 expression responded to abiotic stress in O. basilicum.
- Taxon
- O. basilicumOcimum basilicume0
- Treatment
- abiotic stressese0
- Gene
- ObTLP1e0
- Outcome
- expression of ObTLP1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition, and responsive to biotic and abiotic stresses, and multiple phytohormone elicitations.”
Claim · model-written summary
ObTLP1 expression responded to multiple phytohormone elicitations in O. basilicum.
- Taxon
- O. basilicumOcimum basilicume0
- Treatment
- multiple phytohormone elicitationse0
- Gene
- ObTLP1e0
- Outcome
- expression of ObTLP1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition, and responsive to biotic and abiotic stresses, and multiple phytohormone elicitations.”
Claim · model-written summary
Recombinant ObTLP1 inhibited Scleretonia sclerotiorum mycelial growth.
- Taxon
- Ocimum basilicumOcimum basilicumt0
- Treatment
- Bacterially-expressed recombinant ObTLP1e0
- Interactor
- Scleretonia sclerotiorume0pathogen
- Gene
- ObTLP1e0
- Outcome
- mycelial growth of the phytopathogenic fungie0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacterially-expressed recombinant ObTLP1 inhibited mycelial growth of the phytopathogenic fungi, Scleretonia sclerotiorum and Botrytis cinerea”
t0“Ocimum basilicum”
Claim · model-written summary
Recombinant ObTLP1 inhibited Botrytis cinerea mycelial growth.
- Taxon
- Ocimum basilicumOcimum basilicumt0
- Treatment
- Bacterially-expressed recombinant ObTLP1e0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Gene
- ObTLP1e0
- Outcome
- mycelial growth of the phytopathogenic fungie0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacterially-expressed recombinant ObTLP1 inhibited mycelial growth of the phytopathogenic fungi, Scleretonia sclerotiorum and Botrytis cinerea”
t0“Ocimum basilicum”
Claim · model-written summary
ObTLP1 expression enhanced Arabidopsis tolerance to S. sclerotiorum infection.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Ectopic expression of ObTLP1e0
- Interactor
- S. sclerotiorume0pathogen
- Gene
- ObTLP1e0
- Outcome
- tolerance to S. sclerotiorume0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of ObTLP1 in Arabidopsis led to enhanced tolerance to S. sclerotiorum and B. cinerea infections, and also to dehydration and salt stress.”
Claim · model-written summary
ObTLP1 expression enhanced Arabidopsis tolerance to B. cinerea infection.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Ectopic expression of ObTLP1e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- ObTLP1e0
- Outcome
- B. cinerea infectionse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of ObTLP1 in Arabidopsis led to enhanced tolerance to S. sclerotiorum and B. cinerea infections, and also to dehydration and salt stress.”
Claim · model-written summary
ObTLP1 expression enhanced Arabidopsis tolerance to dehydration.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Ectopic expression of ObTLP1e0
- Gene
- ObTLP1e0
- Outcome
- dehydratione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of ObTLP1 in Arabidopsis led to enhanced tolerance to S. sclerotiorum and B. cinerea infections, and also to dehydration and salt stress.”
Claim · model-written summary
ObTLP1 expression enhanced Arabidopsis tolerance to salt stress.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Ectopic expression of ObTLP1e0
- Gene
- ObTLP1e0
- Outcome
- salt stresse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of ObTLP1 in Arabidopsis led to enhanced tolerance to S. sclerotiorum and B. cinerea infections, and also to dehydration and salt stress.”
Claim · model-written summary
Fungal challenge induced defense marker genes in ObTLP1-expressing Arabidopsis, suggesting activation of defense-response pathways.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- fungal challengee0
- Gene
- defense marker genese0
- Outcome
- up-regulation of the defense-response pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced expression of the defense marker genes suggested up-regulation of the defense-response pathways in ObTLP1-expressing Arabidopsis upon fungal challenge.”
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