Abstract
Somatic embryogenesis and plant regeneration were successfully established on Nitsch and Nitsch(NN69)medium from immature zygotic embryos(IZEs)of interploid crossing 2004-6-12 ×'Aki Queen'.The optimum hormone combinations was NN69+1.80 mg·L-1 NAA+0.4 mg·L-1 casein hydroly sate+2 g·L-1 activated carbon+30 g·L-1 sucrose+6 g·L-1 agar(SE medium)for primary somatic embryogenesis directly from IZEs,with the frequency varying from 11.11% to 16.67% among IZEs of different sampling period after pollination.And importantly,somatic embryogenesis and normal germinating occurred synchronically.While the optimum medium for secondary somatic embryogenesis were 3/4MS+0.35 mg·L-1 IBA+30 g·L-1 sucrose+6 g·L-1 agar(multiplication medium)and MS+1.5 g·L-1 6-BA+0.2 g·L-1 IBA+30 g·L-1 sucrose+6 g·L-1 agar(somatic embryo germination medium).After subcultured on germination medium,somatic embryos converted into normal plantlets.The analysis of DNA content and genetic fidelity determined by flow cytometry and SSR of the regenerated plantlets clearly indicated that the four plantlets were triploid and the normal germinated plant and the somatic embryogenesis-derived plants came from the same immature zygotic embryos.We concluded that somaclonal variation was almost absent in our grapevine plant regeneration system,which was useful in research of transformation and embryo development and so on.
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Claims 8
Claim · model-written summary
Primary somatic embryogenesis from immature zygotic embryos occurred at 11.11% to 16.67% on the optimum medium.
- Organ / stage
- immature zygotic embryose1
- Treatment
- NN69+1.80 mg·L-1 NAA+0.4 mg·L-1 casein hydroly sate+2 g·L-1 activated carbon+30 g·L-1 sucrose+6 g·L-1 agare0
- Outcome
- primary somatic embryogenesise0
- Direction
- optimume0
- Value
- 11.11% to 16.67%e0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine”
e0“The optimum hormone combinations was NN69+1.80 mg·L-1 NAA+0.4 mg·L-1 casein hydroly sate+2 g·L-1 activated carbon+30 g·L-1 sucrose+6 g·L-1 agar(SE medium)for primary somatic embryogenesis directly from IZEs,with the frequency varying from 11.11% to 16.67% among IZEs of different sampling period after pollination.”
e1“immature zygotic embryos(IZEs)”
Claim · model-written summary
Somatic embryogenesis and normal germination occurred synchronously.
- Outcome
- somatic embryogenesis and normal germinating occurred synchronicallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine”
e0“somatic embryogenesis and normal germinating occurred synchronically”
Claim · model-written summary
The stated multiplication medium was optimal for secondary somatic embryogenesis.
- Treatment
- 3/4MS+0.35 mg·L-1 IBA+30 g·L-1 sucrose+6 g·L-1 agare0
- Outcome
- secondary somatic embryogenesise0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine”
e0“the optimum medium for secondary somatic embryogenesis were 3/4MS+0.35 mg·L-1 IBA+30 g·L-1 sucrose+6 g·L-1 agar(multiplication medium)”
Claim · model-written summary
The stated germination medium was optimal for somatic embryo germination.
- Treatment
- MS+1.5 g·L-1 6-BA+0.2 g·L-1 IBA+30 g·L-1 sucrose+6 g·L-1 agare0
- Outcome
- somatic embryo germinatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine”
e0“MS+1.5 g·L-1 6-BA+0.2 g·L-1 IBA+30 g·L-1 sucrose+6 g·L-1 agar(somatic embryo germination medium)”
Claim · model-written summary
Somatic embryos converted into normal plantlets after subculture on germination medium.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine”
e0“After subcultured on germination medium,somatic embryos converted into normal plantlets”
Claim · model-written summary
Four regenerated plantlets were triploid.
- Outcome
- four plantlets were triploide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine”
e0“the four plantlets were triploid”
Claim · model-written summary
The normally germinated and somatic embryo derived plants came from the same immature zygotic embryos.
- Outcome
- normal germinated plant and the somatic embryogenesis-derived plants came from the same immature zygotic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine”
e0“the normal germinated plant and the somatic embryogenesis-derived plants came from the same immature zygotic embryos”
Claim · model-written summary
Somaclonal variation was almost absent in the grapevine regeneration system.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“somaclonal variation was almost absent in our grapevine plant regeneration system”
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