Abstract
In this study,the ThIPK2gene was transformed into the mature seeds of Indica rice Kehui 675 mediated by Agrobacterium.Results showed that callus induction and subculture in all could be finished in 10-14 d in continuous high temperature and illumination,which efficiently shortened the period of callus achieving.The callus induction rate was 95.6 % and the resistant callus induction rate was 10.6 %.The uninterrupted high temperature and illumination accelerated the increase of the resistant calli in 5-7 d during subculture and the differentiation rate was 48.3 %. Transformation rate of the calli which experienced the first subculture was obviously higher than that of calli without subculture.Therefore,the transformation system efficiently shortened the rice transformation period,optimized Indica rice transformation and could provide references for transformating stress-resistant genes into Indica rice.
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Claims 6
Claim · model-written summary
Continuous high temperature and illumination completed callus induction and subculture in 10-14 d and efficiently shortened the callus period.
- Taxon
- Indica rice
Oryza sativa Indica Groupt0 - Treatment
- continuous high temperature and illuminatione1
- Outcome
- period of callus achievinge1
- Direction
- shortenede1
- Value
- 10-14 de1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Kehui 675”
e1“callus induction and subculture in all could be finished in 10-14 d in continuous high temperature and illumination,which efficiently shortened the period of callus achieving”
Claim · model-written summary
The callus induction rate of indica rice Kehui 675 was 95.6 %.
- Taxon
- Indica riceOryza sativa Indica Groupt0
- Outcome
- callus induction ratee2
- Value
- 95.6 %e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Kehui 675”
e2“The callus induction rate was 95.6 %”
Claim · model-written summary
The resistant callus induction rate of indica rice Kehui 675 was 10.6 %.
- Taxon
- Indica riceOryza sativa Indica Groupt0
- Outcome
- resistant callus induction ratee3
- Value
- 10.6 %e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Kehui 675”
e3“the resistant callus induction rate was 10.6 %”
Claim · model-written summary
The differentiation rate of indica rice Kehui 675 was 48.3 %.
- Taxon
- Indica riceOryza sativa Indica Groupt0
- Outcome
- differentiation ratee4
- Value
- 48.3 %e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Kehui 675”
e4“the differentiation rate was 48.3 %”
Claim · model-written summary
Uninterrupted high temperature and illumination accelerated the increase of resistant calli in 5-7 d during subculture.
- Taxon
- Indica riceOryza sativa Indica Groupt0
- Treatment
- uninterrupted high temperature and illuminatione5
- Outcome
- increase of the resistant callie5
- Direction
- acceleratede5
- Value
- 5-7 de5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Kehui 675”
e5“The uninterrupted high temperature and illumination accelerated the increase of the resistant calli in 5-7 d during subculture”
Claim · model-written summary
Calli that experienced the first subculture had obviously higher transformation rate than calli without subculture.
- Taxon
- Indica riceOryza sativa Indica Groupt0
- Organ / stage
- callie6
- Treatment
- first subculturee6
- Outcome
- Transformation ratee6
- Direction
- highere6
- Comparator
- calli without subculturee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Kehui 675”
e6“Transformation rate of the calli which experienced the first subculture was obviously higher than that of calli without subculture”
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