Abstract
Five important genes coding for nitrate reductase(NR),nitrite reductase(NiR),glutamine synthetase(GS),glutamate dehydrogenase(GDH) and asparagine synthetase(AS),were cloned from the related EST sequences deposited in VitisEST database and amplified using RT-PCR.Sub-cellular localization was employed to characterize their functional position,while semi-quantitative RT-PCR and fluorescent quantitative PCR were both used to analyze their expression in leaves after treatment with urea at different concentrations.Results show that the five genes had significantly higher expression in younger leaves compared to old leaves.The differences in expression of these five genes at different times were inconsistent,but generally expression was increased by application of foliar urea,with application of 0.3% and 0.5% urea causing significant increases in gene expression compared to control plants.The expression of the five genes related to foliar fertilizer application was corroborated by the significant increases in related fruit indicators.Expression of GS in grape leaves had the most significant increase at 6 h after spraying of urea,while NR maintained a relatively high level of expression even at 48h after urea application compared to the other genes.NR and GS expression in various stages was higher than NiR and AS respectively,with the trends in expression of NiR and AS being consistent with those of NR and GS respectively.This study provides a theoretical basis for further studies on the mechanisms of nitrogen metabolic pathways and scientifically informed foliar fertilizer application.
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Claims 11
Claim · model-written summary
Five nitrogen metabolism genes were cloned from grapevine EST sequences.
Verbatim
e0“Five important genes coding for nitrate reductase(NR),nitrite reductase(NiR),glutamine synthetase(GS),glutamate dehydrogenase(GDH) and asparagine synthetase(AS),were cloned from the related EST sequences deposited in VitisEST database”
t0“Fujiminori grapevine”
Claim · model-written summary
All five genes had higher expression in younger than old leaves.
Verbatim
e0“the five genes had significantly higher expression in younger leaves compared to old leaves”
t0“Fujiminori grapevine”
Claim · model-written summary
Foliar urea generally increased expression of the five genes.
Not reportedComparator
Verbatim
e0“generally expression was increased by application of foliar urea”
t0“Fujiminori grapevine”
Claim · model-written summary
0.3% urea significantly increased gene expression over control.
Verbatim
e0“application of 0.3% and 0.5% urea causing significant increases in gene expression compared to control plants”
t0“Fujiminori grapevine”
Claim · model-written summary
0.5% urea significantly increased gene expression over control.
Verbatim
e0“application of 0.3% and 0.5% urea causing significant increases in gene expression compared to control plants”
t0“Fujiminori grapevine”
Claim · model-written summary
Related fruit indicators significantly increased with foliar fertilizer.
- Taxon
- Fujiminori grapevinet0
- Treatment
- foliar fertilizer applicatione0
- Outcome
- related fruit indicatorse0
- Direction
- increasese0
Not reportedComparator
Verbatim
e0“The expression of the five genes related to foliar fertilizer application was corroborated by the significant increases in related fruit indicators”
t0“Fujiminori grapevine”
Claim · model-written summary
GS expression increased most at 6 h after urea spray.
- Taxon
- grapee0
- Treatment
- spraying of ureae0
- Gene
- GSe0
- Outcome
- Expression of GSe0
- Direction
- increasee0
- Qualifier
- 6 he0
Not reportedComparator
Verbatim
e0“Expression of GS in grape leaves had the most significant increase at 6 h after spraying of urea”
Claim · model-written summary
NR expression remained relatively high at 48h after urea application.
Verbatim
e0“NR maintained a relatively high level of expression even at 48h after urea application compared to the other genes”
t0“Fujiminori grapevine”
Claim · model-written summary
NR expression was higher than NiR expression across stages.
Verbatim
e0“NR and GS expression in various stages was higher than NiR and AS respectively”
t0“Fujiminori grapevine”
Claim · model-written summary
GS expression was higher than AS expression across stages.
Verbatim
e0“NR and GS expression in various stages was higher than NiR and AS respectively”
t0“Fujiminori grapevine”
Claim · model-written summary
NiR and AS expression followed the respective trends of NR and GS.
Not reportedDirection
Verbatim
e0“the trends in expression of NiR and AS being consistent with those of NR and GS respectively”
t0“Fujiminori grapevine”
The same questions in other papers
nitrogen · molecular: expression · VitisPositive and negative results
1 in the opposite direction
- no effect
application · molecular: expression · VitisPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.