Abstract
The mechanisms of H 2O 2 generation in guard cells of Vicia faba induced by abscisic acid (ABA) were measured by using fluorescene probe, 8 hydroxypyrene 1 ,3,6 trisulfonic acid, trisodium salt (HPTS). When guard cells were treated with ABA, fluorescene quenching of HPTS occurred, and the treatment of the guard cells with diphenyleneiodonium(DPI) at 10 -6 mol/mL (final concentration) which is known as an inhibitor of supero xide generating NADPH oxidases nearly reversed the quenching reaction induced by ABA 0 2 mmol/L(final concentration). ABA and H 2O 2 might induce stomatal closure. Furthermore, when DPI or catalase(CAT)( 10 3 unit/mL ) was added to the suspension after above treatment reversed ABA induced stomatal closure. These results suggest that a novel O 2 generating NADPH oxidase in the plasma membrane of guard cell of Vicia faba may be activated by ABA, and subsequently H 2O 2, which involved in the signal transduction pathway of ABA induced stomatal closure.
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Claims 6
Claim · model-written summary
ABA treatment quenched HPTS fluorescence in Vicia faba guard cells.
- Taxon
- Vicia faba
Vicia fabat0 - Organ / stage
- guard cellse0
- Treatment
- ABAe0
- Outcome
- fluorescene quenching of HPTSe0
- Direction
- occurrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When guard cells were treated with ABA, fluorescene quenching of HPTS occurred”
t0“Vicia faba”
Claim · model-written summary
DPI nearly reversed ABA induced HPTS quenching in guard cells.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- diphenyleneiodonium(DPI) at 10 -6 mol/mLe0
- Gene
- NADPH oxidasese0
- Outcome
- quenching reactione0
- Direction
- nearly reversede0
- Comparator
- ABA 0 2 mmol/Le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment of the guard cells with diphenyleneiodonium(DPI) at 10 -6 mol/mL (final concentration) which is known as an inhibitor of supero xide generating NADPH oxidases nearly reversed the quenching reaction induced by ABA 0 2 mmol/L(final concentration)”
t0“Vicia faba”
Claim · model-written summary
ABA and hydrogen peroxide may induce stomatal closure.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- ABA and H 2O 2e0
- Outcome
- stomatal closuree0
- Qualifier
- might inducee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA and H 2O 2 might induce stomatal closure.”
t0“Vicia faba”
Claim · model-written summary
DPI reversed ABA induced stomatal closure.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- DPIe0
- Outcome
- stomatal closuree0
- Direction
- reversede0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when DPI or catalase(CAT)( 10 3 unit/mL ) was added to the suspension after above treatment reversed ABA induced stomatal closure”
t0“Vicia faba”
Claim · model-written summary
Catalase reversed ABA induced stomatal closure.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- catalase(CAT)( 10 3 unit/mL )e0
- Outcome
- stomatal closuree0
- Direction
- reversede0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“catalase(CAT)( 10 3 unit/mL ) was added to the suspension after above treatment reversed ABA induced stomatal closure”
t0“Vicia faba”
Claim · model-written summary
The authors suggest that ABA activates guard cell NADPH oxidase, producing hydrogen peroxide for stomatal closure signaling.
- Taxon
- Vicia fabaVicia fabae0
- Treatment
- ABAe0
- Gene
- NADPH oxidasee0
- Outcome
- stomatal closuree0
- Qualifier
- may be activatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel O 2 generating NADPH oxidase in the plasma membrane of guard cell of Vicia faba may be activated by ABA, and subsequently H 2O 2, which involved in the signal transduction pathway of ABA induced stomatal closure”
The same questions in other papers
dibenziodolium · physiology: gas exchange · Vicia faba (faba bean)Results differ
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.