Abstract
Fusicoccin (FC) treatment prevents dark-induced stomatal closure, the mechanism of which is still obscure. By using pharmacological approaches and laser-scanning confocal microscopy, the relationship between FC inhibition of dark-induced stomatal closure and the hydrogen peroxide (H₂O₂) levels in guard cells in broad bean was studied. Like ascorbic acid (ASA), a scavenger of H₂O₂ and diphenylene iodonium (DPI), an inhibitor of H₂O₂-generating enzyme NADPH oxidase, FC was found to inhibit stomatal closure and reduce H₂O₂ levels in guard cells in darkness, indicating that FC-caused inhibition of dark-induced stomatal closure is related to the reduction of H₂O₂ levels in guard cells. Furthermore, like ASA, FC not only suppressed H₂O₂-induced stomatal closure and H₂O₂ levels in guard cells treated with H₂O₂ in light, but also reopened the stomata which had been closed by darkness and reduced the level of H₂O₂ that had been generated by darkness, showing that FC causes H₂O₂ removal in guard cells. The butyric acid treatment simulated the effects of FC on the stomata treated with H₂O₂ and had been closed by dark, and on H₂O₂ levels in guard cells of stomata treated with H₂O₂ and had been closed by dark, and both FC and butyric acid reduced cytosol pH in guard cells of stomata treated with H₂O₂ and had been closed by dark, which demonstrates that cytosolic acidification mediates FC-induced H₂O₂ removal. Taken together, our results provide evidence that FC causes cytosolic acidification, consequently induces H₂O₂ removal, and finally prevents dark-induced stomatal closure.
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Claims 11
Claim · model-written summary
Fusicoccin inhibited stomatal closure in darkness.
- Taxon
- Vicia faba
Vicia fabat0 - Treatment
- FCe0
- Outcome
- stomatal closuree0
- Direction
- inhibite0
- Setting
- darknesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“FC was found to inhibit stomatal closure and reduce H₂O₂ levels in guard cells in darkness”
Claim · model-written summary
Fusicoccin reduced guard-cell hydrogen peroxide in darkness.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- FCe0
- Outcome
- H₂O₂ levels in guard cellse0
- Direction
- reducee0
- Setting
- darknesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“FC was found to inhibit stomatal closure and reduce H₂O₂ levels in guard cells in darkness”
Claim · model-written summary
Ascorbic acid inhibited stomatal closure and reduced guard-cell peroxide in darkness.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- ascorbic acid (ASA)e0
- Outcome
- stomatal closure and reduce H₂O₂ levels in guard cellse0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“Like ascorbic acid (ASA), a scavenger of H₂O₂ and diphenylene iodonium (DPI), an inhibitor of H₂O₂-generating enzyme NADPH oxidase, FC was found to inhibit stomatal closure and reduce H₂O₂ levels in guard cells in darkness”
Claim · model-written summary
Diphenylene iodonium inhibited stomatal closure and reduced guard-cell peroxide in darkness.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- diphenylene iodonium (DPI)e0
- Outcome
- stomatal closure and reduce H₂O₂ levels in guard cellse0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“Like ascorbic acid (ASA), a scavenger of H₂O₂ and diphenylene iodonium (DPI), an inhibitor of H₂O₂-generating enzyme NADPH oxidase, FC was found to inhibit stomatal closure and reduce H₂O₂ levels in guard cells in darkness”
Claim · model-written summary
Fusicoccin suppressed peroxide-induced stomatal closure in light.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- FCe0
- Outcome
- H₂O₂-induced stomatal closuree0
- Direction
- suppressede0
- Setting
- lighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“FC not only suppressed H₂O₂-induced stomatal closure and H₂O₂ levels in guard cells treated with H₂O₂ in light”
Claim · model-written summary
Fusicoccin suppressed guard-cell peroxide under peroxide treatment in light.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- FCe0
- Outcome
- H₂O₂ levels in guard cellse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“FC not only suppressed H₂O₂-induced stomatal closure and H₂O₂ levels in guard cells treated with H₂O₂ in light”
Claim · model-written summary
Fusicoccin reopened stomata closed by darkness.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- FCe0
- Outcome
- stomata which had been closed by darknesse0
- Direction
- reopenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“FC not only suppressed H₂O₂-induced stomatal closure and H₂O₂ levels in guard cells treated with H₂O₂ in light, but also reopened the stomata which had been closed by darkness”
Claim · model-written summary
Fusicoccin reduced guard-cell peroxide generated by darkness.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- FCe1
- Outcome
- level of H₂O₂e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“reduced the level of H₂O₂ that had been generated by darkness”
e1“FC not only suppressed H₂O₂-induced stomatal closure”
Claim · model-written summary
Butyric acid simulated fusicoccin effects on peroxide-treated or dark-closed stomata and guard-cell peroxide.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- butyric acid treatmente0
- Outcome
- stomata treated with H₂O₂ and had been closed by dark, and on H₂O₂ levels in guard cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“The butyric acid treatment simulated the effects of FC on the stomata treated with H₂O₂ and had been closed by dark, and on H₂O₂ levels in guard cells of stomata treated with H₂O₂ and had been closed by dark”
Claim · model-written summary
Fusicoccin and butyric acid lowered guard-cell cytosolic pH.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- FC and butyric acide0
- Outcome
- cytosol pH in guard cellse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“both FC and butyric acid reduced cytosol pH in guard cells of stomata treated with H₂O₂ and had been closed by dark”
Claim · model-written summary
The authors infer that cytosolic acidification mediates fusicoccin-induced peroxide removal.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- FCe0
- Outcome
- H₂O₂ removale0
- Qualifier
- cytosolic acidificatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e0“cytosolic acidification mediates FC-induced H₂O₂ removal”
The same questions in other papers
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1 paper in the same direction as this paper · 3 in the opposite direction
- negative
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See all 5 papers →fusicoccin · physiology: gas exchange · Vicia faba (faba bean)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
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ascorbic acid · physiology: gas exchange · Vicia faba (faba bean)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.