Abstract
The fiber induction and development from cotton ovules of fiber mutants in vitro were studied and analyzed. The results showed that there were great differences in the fiber development for genotypes, the fiber inducing percentage and the fiber quantity were less than TM 1. Ability of fiber inducement in vitro from ovule of different mutants can be put in order: TM 1LiN 1T 586H 10 .Although Fiberless Xu Zhou142 (fl) had not ability to differentiate fiber and fuzz in vivo, it could be induced to produce a lot of fiber like cells from epidermal cells in Bt medium. Some hormones, such as IAA and GA 3, were necessary to induce fibers in vitro, The Bt medium with 5.0μmol·L 1 IAA and 0.5μmol·L 1 GA 3 was better for fiber induction and the development. The effects of hormonal concentration might be differential in different fiber mutants. GA 3 could not only promote expansion of ovule, but also easily induce ovule into callus. Fiberless Xu Zhou142 (fl) and T 586 were easier for callus induction than the other mutants, they had hightest callus frequency in Bt medium with 0.5μmol IAA and 5.0μmol·L 1 GA 3.
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Claims 9
Claim · model-written summary
Fiber induction percentage and fiber quantity differed by genotype and were below TM 1.
- Taxon
- cotton
Gossypium hirsutumt0 - Material
- genotypese0
- Outcome
- fiber inducing percentage and the fiber quantitye0
- Direction
- less thane0
- Comparator
- TM 1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were great differences in the fiber development for genotypes, the fiber inducing percentage and the fiber quantity were less than TM 1”
t0“Cotton Ovules”
Claim · model-written summary
Fiberless Xu Zhou142 lacked in vivo fiber and fuzz differentiation.
- Taxon
- CottonGossypium hirsutumt0
- Material
- Fiberless Xu Zhou142 (fl)e0
- Outcome
- not ability to differentiate fiber and fuzze0
- Setting
- in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fiberless Xu Zhou142 (fl) had not ability to differentiate fiber and fuzz in vivo, it could be induced to produce a lot of fiber like cells from epidermal cells in Bt medium”
t0“Cotton Ovules”
Claim · model-written summary
Bt medium induced fiber like cells from Fiberless Xu Zhou142 epidermis.
- Taxon
- CottonGossypium hirsutumt0
- Material
- Fiberless Xu Zhou142 (fl)e0
- Treatment
- Bt mediume0
- Outcome
- produce a lot of fiber like cellse0
- Direction
- inducede0
- Comparator
- in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fiberless Xu Zhou142 (fl) had not ability to differentiate fiber and fuzz in vivo, it could be induced to produce a lot of fiber like cells from epidermal cells in Bt medium”
t0“Cotton Ovules”
Claim · model-written summary
IAA and GA 3 were necessary for in vitro fiber induction.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- IAA and GA 3e0
- Outcome
- induce fiberse0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some hormones, such as IAA and GA 3, were necessary to induce fibers in vitro”
t0“Cotton Ovules”
Claim · model-written summary
Bt medium with IAA and GA 3 improved fiber induction and development.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Bt medium with 5.0μmol·L 1 IAA and 0.5μmol·L 1 GA 3e0
- Outcome
- fiber induction and the developmente0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Bt medium with 5.0μmol·L 1 IAA and 0.5μmol·L 1 GA 3 was better for fiber induction and the development”
t0“Cotton Ovules”
Claim · model-written summary
GA 3 promoted ovule expansion.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- GA 3e0
- Outcome
- expansion of ovulee0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA 3 could not only promote expansion of ovule, but also easily induce ovule into callus”
t0“Cotton Ovules”
Claim · model-written summary
GA 3 induced ovule callus.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- GA 3e0
- Outcome
- induce ovule into calluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA 3 could not only promote expansion of ovule, but also easily induce ovule into callus”
t0“Cotton Ovules”
Claim · model-written summary
Fiberless Xu Zhou142 (fl) had the highest callus frequency in the specified Bt medium.
- Taxon
- CottonGossypium hirsutumt0
- Material
- Fiberless Xu Zhou142 (fl)e0
- Treatment
- Bt medium with 0.5μmol IAA and 5.0μmol·L 1 GA 3e0
- Outcome
- callus frequencye0
- Direction
- highteste0
- Comparator
- the other mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fiberless Xu Zhou142 (fl) and T 586 were easier for callus induction than the other mutants, they had hightest callus frequency in Bt medium with 0.5μmol IAA and 5.0μmol·L 1 GA 3”
t0“Cotton Ovules”
Claim · model-written summary
T 586 had the highest callus frequency in the specified Bt medium.
- Taxon
- CottonGossypium hirsutumt0
- Material
- T 586e0
- Treatment
- Bt medium with 0.5μmol IAA and 5.0μmol·L 1 GA 3e0
- Outcome
- callus frequencye0
- Direction
- highteste0
- Comparator
- the other mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fiberless Xu Zhou142 (fl) and T 586 were easier for callus induction than the other mutants, they had hightest callus frequency in Bt medium with 0.5μmol IAA and 5.0μmol·L 1 GA 3”
t0“Cotton Ovules”
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