Abstract
This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.
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Claims 8
Claim · model-written summary
Virus-induced silencing of NbRanBP1 caused stunted growth, leaf yellowing and abnormal leaf morphology.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- Virus-induced gene silencing (VIGS) of NbRanBP1e0
- Gene
- NbRanBP1e0
- Outcome
- stunted growth, leaf yellowing, and abnormal leaf morphologye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e0“Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology”
Claim · model-written summary
NbRanBP1 is constitutively expressed in diverse tissues.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- diverse tissuese1
- Gene
- NbRanBP1e1
- Outcome
- constitutively expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“The NbRanBP1 gene was constitutively expressed in diverse tissues”
Claim · model-written summary
The NbRanBP1:GFP fusion protein is primarily localized to the nuclear rim and the cytosol.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbRanBP1:GFPe2
- Outcome
- primarily localized to the nuclear rim and the cytosole2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e2“an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol”
Claim · model-written summary
NbRanBP1 interacts with NbRan1 in vivo in the nuclear envelope and the cytosol.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbRanBP1e3
- Outcome
- in vivo interactione3
- Qualifier
- NbRan1 in the nuclear envelope and the cytosole3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e3“BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol”
Claim · model-written summary
Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Depletion of NbRanBP1 or NbRan1e4
- Gene
- NbBTF3:GFPe4
- Outcome
- nuclear accumulation of a NbBTF3:GFP marker proteine4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e4“Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein”
Claim · model-written summary
In later stages of development, NbRanBP1 VIGS plants show reduced mitochondrial membrane potential.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- later stages of developmente5
- Treatment
- NbRanBP1 VIGSe5
- Outcome
- mitochondrial membrane potentiale5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e5“In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes”
Claim · model-written summary
In later stages of development, NbRanBP1 VIGS plants show excessive production of reactive oxygen species.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- later stages of developmente5
- Treatment
- NbRanBP1 VIGSe5
- Outcome
- reactive oxygen speciese5
- Direction
- excessive productione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e5“In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes”
Claim · model-written summary
In later stages of development, NbRanBP1 VIGS plants show induction of defense-related genes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- later stages of developmente5
- Treatment
- NbRanBP1 VIGSe5
- Outcome
- defense-related genese5
- Direction
- inductione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e5“In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes”
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