Abstract
Summary. Various nitrogen compounds were tested for their ability to alleviate the reduced nitrogen requirement of soybean cells growing in defined liquid medium containing nitrate as the alternative nitrogen source. Either L-glutamine, L-alanine, putrescine or NH4+ satisfied this requirement. Addition of L-glutamate resulted in poor growth. Where growth was stimulated, nitrate reductase (NR) activity increased whereas glutamate dehydrogenase activity in the cells showed no such correlation. In all fresh media which supported rapid growth, NR activity first decreased rapidly to a low value. Subsequent dry weight increases occurred con commitantly with an increase in NR activity. When 2,4-dichlorophenoxyacetic acid was omitted from the medium the growth was slow and the NR activity did not increase. During the first 40 h of incubation in medium containing NH4+ plus NO3- the cells produced a growth-enhancing factor (s). This factor(s) was present in the cells and in the medium and eliminated the requirement for reduced nitrogen.
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Claims 11
Claim · model-written summary
L-glutamine, L-alanine, putrescine and NH4+ each satisfied the reduced nitrogen requirement of soybean cells.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- L-glutamine, L-alanine, putrescine or NH4+e0
- Outcome
- satisfied this requiremente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Either L-glutamine, L-alanine, putrescine or NH4+ satisfied this requirement.”
t0“Soybean”
Claim · model-written summary
L-glutamate led to poor soybean cell growth.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- L-glutamatee0
- Outcome
- poor growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of L-glutamate resulted in poor growth.”
t0“Soybean”
Claim · model-written summary
Where soybean cell growth was stimulated, nitrate reductase activity increased.
- Taxon
- SoybeanGlycine maxt0
- Gene
- nitrate reductase (NR)e0
- Outcome
- nitrate reductase (NR) activitye0
- Direction
- increasede0
- Comparator
- growth was stimulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Where growth was stimulated, nitrate reductase (NR) activity increased”
t0“Soybean”
Claim · model-written summary
Glutamate dehydrogenase activity did not correlate with stimulated soybean cell growth.
- Taxon
- SoybeanGlycine maxt0
- Gene
- glutamate dehydrogenasee0
- Outcome
- glutamate dehydrogenase activitye0
- Qualifier
- no such correlatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glutamate dehydrogenase activity in the cells showed no such correlation”
e1“Where growth was stimulated”
t0“Soybean”
Claim · model-written summary
In fresh media supporting rapid soybean cell growth, NR activity initially fell rapidly to a low value.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- NR activitye0
- Direction
- decreasede0
- Qualifier
- fresh media which supported rapid growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In all fresh media which supported rapid growth, NR activity first decreased rapidly to a low value.”
t0“Soybean”
Claim · model-written summary
Subsequent soybean cell dry weight increases coincided with increasing NR activity.
- Taxon
- SoybeanGlycine maxt0
- Gene
- NRe0
- Outcome
- dry weighte0
- Direction
- increasese0
- Qualifier
- con commitantly with an increase in NR activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subsequent dry weight increases occurred con commitantly with an increase in NR activity.”
t0“Soybean”
Claim · model-written summary
Omission of 2,4-dichlorophenoxyacetic acid slowed soybean cell growth.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 2,4-dichlorophenoxyacetic acid was omittede0
- Outcome
- growthe0
- Direction
- slowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When 2,4-dichlorophenoxyacetic acid was omitted from the medium the growth was slow”
t0“Soybean”
Claim · model-written summary
When 2,4-dichlorophenoxyacetic acid was omitted, soybean cell NR activity did not increase.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 2,4-dichlorophenoxyacetic acid was omittede0
- Outcome
- NR activitye0
- Direction
- did not increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When 2,4-dichlorophenoxyacetic acid was omitted from the medium the growth was slow and the NR activity did not increase.”
t0“Soybean”
Claim · model-written summary
Soybean cells incubated with NH4+ plus NO3- produced a growth enhancing factor during the first 40 hours.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- produced a growth-enhancing factor (s)e0
- Qualifier
- first 40 h of incubation in medium containing NH4+ plus NO3-e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During the first 40 h of incubation in medium containing NH4+ plus NO3- the cells produced a growth-enhancing factor (s).”
t0“Soybean”
Claim · model-written summary
The soybean cell growth enhancing factor was present in cells and medium.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- present in the cells and in the mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This factor(s) was present in the cells and in the medium”
t0“Soybean”
Claim · model-written summary
The soybean cell growth enhancing factor eliminated the requirement for reduced nitrogen.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- eliminated the requirement for reduced nitrogene0
- Qualifier
- This factor(s)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This factor(s) was present in the cells and in the medium and eliminated the requirement for reduced nitrogen.”
t0“Soybean”
The same questions in other papers
2,4-D · growth · Glycine max (soybean)Results differ
14 papers in the same direction as this paper · 20 in the opposite direction
- positive
- positive
- positive
31 papers more in this group
See all 35 papers →2,4-D · physiology: enzyme activity · Glycine max (soybean)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.