Abstract
Selected factors affecting somatic embryogenesis efficiency have been studied, namely genotype, explant type and its orientation in the medium, different basal media, different auxins for somatic embryo induction, and two ways of donor plant cultivation. The key role is played by genotype and auxin used, the minimum effect was observed due to basal media. In the series of subsequent experiments we have found the best combination of individual factors as follows: cv. Altona, 10 uM 2,4-D, L2 basal medium, central part of immature cotyledon as initial expiant oriented by adaxial side down on the agar medium, and field grown donor plants. This combination exhibited 100 % embryogenic explants with 5.43 ± 0.65 somatic embryos per expiant,i.e. somatic embryogenesis efficiency 5.43.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Genotype and auxin were the key factors in somatic embryogenesis efficiency.
- Taxon
- soybean (Glycine max (L.) Merr.)
Glycine maxt0 - Treatment
- auxin usede0
- Outcome
- key rolee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The key role is played by genotype and auxin used”
t0“soybean (Glycine max (L.) Merr.)”
Claim · model-written summary
Basal media had the smallest observed effect on somatic embryogenesis efficiency.
- Taxon
- soybean (Glycine max (L.) Merr.)Glycine maxt0
- Treatment
- basal mediae0
- Outcome
- somatic embryogenesis efficiencyt0
- Direction
- minimume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the minimum effect was observed due to basal media”
t0“somatic embryogenesis efficiency in soybean (Glycine max (L.) Merr.)”
Claim · model-written summary
The selected factor combination yielded 100 % embryogenic explants.
- Taxon
- soybean (Glycine max (L.) Merr.)Glycine maxt0
- Material
- cv. Altonae0
- Treatment
- 10 uM 2,4-D, L2 basal medium, central part of immature cotyledon as initial expiant oriented by adaxial side down on the agar medium, and field grown donor plantse0
- Outcome
- embryogenic explantse0
- Value
- 100 %e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cv. Altona, 10 uM 2,4-D, L2 basal medium, central part of immature cotyledon as initial expiant oriented by adaxial side down on the agar medium, and field grown donor plants. This combination exhibited 100 % embryogenic explants with 5.43 ± 0.65 somatic embryos per expiant,i.e. somatic embryogenesis efficiency 5.43.”
t0“soybean (Glycine max (L.) Merr.)”
Claim · model-written summary
The selected factor combination produced 5.43 ± 0.65 somatic embryos per explant.
- Taxon
- soybean (Glycine max (L.) Merr.)Glycine maxt0
- Material
- cv. Altonae0
- Treatment
- 10 uM 2,4-D, L2 basal medium, central part of immature cotyledon as initial expiant oriented by adaxial side down on the agar medium, and field grown donor plantse0
- Outcome
- somatic embryos per expiante0
- Value
- 5.43 ± 0.65 somatic embryos per expiante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cv. Altona, 10 uM 2,4-D, L2 basal medium, central part of immature cotyledon as initial expiant oriented by adaxial side down on the agar medium, and field grown donor plants. This combination exhibited 100 % embryogenic explants with 5.43 ± 0.65 somatic embryos per expiant,i.e. somatic embryogenesis efficiency 5.43.”
t0“soybean (Glycine max (L.) Merr.)”
Claim · model-written summary
The selected factor combination had somatic embryogenesis efficiency 5.43.
- Taxon
- soybean (Glycine max (L.) Merr.)Glycine maxt0
- Outcome
- somatic embryogenesis efficiencye0
- Value
- 5.43e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“somatic embryogenesis efficiency 5.43”
t0“soybean (Glycine max (L.) Merr.)”
The same questions in other papers
2,4-D · growth · Glycine max (soybean)Results differ
19 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- negative
31 papers more in this group
See all 35 papers →in vitro · growth · Glycine max (soybean)Positive and negative results
37 papers in the same direction as this paper · 14 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
49 papers more in this group
See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.