Abstract
The effect of germination on the protein content and in the level of specific activity of lipoxygenase-1 on seedlings of Santa Rosa, FT-2 and Davis soybean cultivars were studied. Soybean seeds of the three cultivars were germinated from 0 to 72 hours in a seed germinator at 30 degrees C temperature, 100% relative humidity, and absence of light. The seeds were then frozen in liquid nitrogen, freeze-dried and milled. The lipoxygenase-1 was extracted with 0.02M thris-HCI buffer at pH = 8.2 and 13% saccharose for 30 minutes at 4 degrees C without shaking. The highest levels of specific activity of lipoxygenase-1 per milligram of flour (dry basis) in the Santa Rosa, FT-2 and Davis cultivars were observed between 18 and 36, 18 and 24, and 0 and 12 hours, respectively. The polinomial quadratic model best fits the regression analysis of the variable levels of specific lipoxygenase activity for cultivar Davis (R2 = 0.9197). Although for cultivars Santa Rosa (R2 = 0.9041)., and FT-2 (R2 = 0.7486) the 4th degree polynomial model has shown the best fit. The protein content in the three cultivars studied reached the maximum values at 48h germination. The germination induced a relative increase of the protein content but caused a reduction of the level of specific lipoxygenase-1 activity.
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Claims 9
Claim · model-written summary
Santa Rosa had peak specific lipoxygenase-1 activity between 18 and 36 hours.
- Taxon
- soybean
Glycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest levels of specific activity of lipoxygenase-1 per milligram of flour (dry basis) in the Santa Rosa, FT-2 and Davis cultivars were observed between 18 and 36, 18 and 24, and 0 and 12 hours, respectively.”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
FT-2 had peak specific lipoxygenase-1 activity between 18 and 24 hours.
- Taxon
- soybeanGlycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest levels of specific activity of lipoxygenase-1 per milligram of flour (dry basis) in the Santa Rosa, FT-2 and Davis cultivars were observed between 18 and 36, 18 and 24, and 0 and 12 hours, respectively.”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
Davis had peak specific lipoxygenase-1 activity between 0 and 12 hours.
- Taxon
- soybeanGlycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest levels of specific activity of lipoxygenase-1 per milligram of flour (dry basis) in the Santa Rosa, FT-2 and Davis cultivars were observed between 18 and 36, 18 and 24, and 0 and 12 hours, respectively.”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
A quadratic model best fit Davis lipoxygenase activity data.
- Taxon
- soybeanGlycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polinomial quadratic model best fits the regression analysis of the variable levels of specific lipoxygenase activity for cultivar Davis (R2 = 0.9197).”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
A fourth-degree polynomial best fit Santa Rosa lipoxygenase activity data.
- Taxon
- soybeanGlycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“for cultivars Santa Rosa (R2 = 0.9041)., and FT-2 (R2 = 0.7486) the 4th degree polynomial model has shown the best fit.”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
A fourth-degree polynomial best fit FT-2 lipoxygenase activity data.
- Taxon
- soybeanGlycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“for cultivars Santa Rosa (R2 = 0.9041)., and FT-2 (R2 = 0.7486) the 4th degree polynomial model has shown the best fit.”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
Protein content in all three cultivars peaked at 48 hours of germination.
- Taxon
- soybeanGlycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protein content in the three cultivars studied reached the maximum values at 48h germination.”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
Germination increased soybean protein content.
- Taxon
- soybeanGlycine maxe0t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germination induced a relative increase of the protein content but caused a reduction of the level of specific lipoxygenase-1 activity.”
t0“seedlings of three soybean cultivars”
Claim · model-written summary
Germination reduced specific lipoxygenase-1 activity.
- Taxon
- soybeanGlycine maxe0t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germination induced a relative increase of the protein content but caused a reduction of the level of specific lipoxygenase-1 activity.”
t0“seedlings of three soybean cultivars”
The same questions in other papers
processing · quality: protein · Glycine max (soybean)Positive and negative results
9 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.