Abstract
In humans, CHMP1 encodes a protein of dual function that plays a role in both modification of chromatin structure and endosomal vesicle trafficking. Recently, it was found that sal1, a CHMP1 homolog in maize, is important for the development of the aleurone cell layers in maize endosperm. In this study, we investigated the structure and function of a Nicotiana benthamiana CHMP1 homolog designated NbCHMP1. NbCHMP1 encodes a small protein with a bipartite nuclear localization signal at its N-terminus, and good homology with the corresponding genes from diverse plants and animals. NbCHMP1 mRNA was present at comparable levels in stems, roots, flowers, and leaves. A GFP fusion of the full length NbCHMP1 protein was localized to the cytosol in distinct structures, while a GFP fusion of its N-terminal 80 aa was targeted to the nucleus, suggesting dual-targeting of NbCHMP1 in plants. Overexpression of NbCHMP1 in yeast did not affect its growth and the expressed protein was present in the cytosol in particulate form. Virus-induced gene silencing of NbCHMP1 resulted in subtle alterations of leaf morphology and color, without significantly affecting plant viability or development. Thus the CHMP1 homolog apparently does not play an essential role in the development of the vegetative tissues of N. benthamiana, in contrast to the essential role of sail in formation of the aleurone cell layers during maize endosperm development.
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Claims 10
Claim · model-written summary
NbCHMP1 encodes a small protein with a bipartite nuclear localization signal.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Gene
- NbCHMP1e1
- Outcome
- small protein with a bipartite nuclear localization signale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“NbCHMP1 encodes a small protein with a bipartite nuclear localization signal at its N-terminus”
Claim · model-written summary
NbCHMP1 mRNA was present at comparable levels in stems, roots, flowers and leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- stems, roots, flowers, and leavese1
- Gene
- NbCHMP1e1
- Outcome
- NbCHMP1 mRNAe1
- Qualifier
- comparable levelse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“NbCHMP1 mRNA was present at comparable levels in stems, roots, flowers, and leaves”
Claim · model-written summary
The full-length NbCHMP1 GFP fusion localized to the cytosol in distinct structures.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbCHMP1e1
- Outcome
- localized to the cytosole1
- Qualifier
- in distinct structurese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“A GFP fusion of the full length NbCHMP1 protein was localized to the cytosol in distinct structures”
Claim · model-written summary
The N-terminal 80 aa of NbCHMP1 fused to GFP targeted to the nucleus.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- targeted to the nucleuse1
- Qualifier
- N-terminal 80 aae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“while a GFP fusion of its N-terminal 80 aa was targeted to the nucleus”
Claim · model-written summary
NbCHMP1 shows dual targeting in plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbCHMP1e1
- Outcome
- dual-targetinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“suggesting dual-targeting of NbCHMP1 in plants”
Claim · model-written summary
Overexpression of NbCHMP1 in yeast did not affect yeast growth.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Overexpression of NbCHMP1 in yeaste1
- Gene
- NbCHMP1e1
- Outcome
- growthe1
- Direction
- did not affecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“Overexpression of NbCHMP1 in yeast did not affect its growth”
Claim · model-written summary
The expressed NbCHMP1 protein in yeast was present in the cytosol in particulate form.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- present in the cytosol in particulate forme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“the expressed protein was present in the cytosol in particulate form”
Claim · model-written summary
Virus-induced silencing of NbCHMP1 caused subtle alterations of leaf morphology and color.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Virus-induced gene silencing of NbCHMP1e1
- Gene
- NbCHMP1e1
- Outcome
- leaf morphology and colore1
- Qualifier
- subtle alterationse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“Virus-induced gene silencing of NbCHMP1 resulted in subtle alterations of leaf morphology and color, without significantly affecting plant viability or development”
Claim · model-written summary
Virus-induced silencing of NbCHMP1 did not significantly affect plant viability or development.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Virus-induced gene silencing of NbCHMP1e1
- Outcome
- plant viability or developmente1
- Direction
- without significantly affectinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“Virus-induced gene silencing of NbCHMP1 resulted in subtle alterations of leaf morphology and color, without significantly affecting plant viability or development”
Claim · model-written summary
The CHMP1 homolog apparently does not play an essential role in the development of vegetative tissues of N. benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- CHMP1 homologe1
- Outcome
- essential role in the development of the vegetative tissuese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Molecular Characterization of NbCHMP1 Encoding a Homolog of Human CHMP1 in Nicotiana benthamiana”
e1“Thus the CHMP1 homolog apparently does not play an essential role in the development of the vegetative tissues of N. benthamiana”
The same questions in other papers
loss of function · growth · Nicotiana benthamianaPositive and negative results
16 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.