Abstract
Two tobacco cell lines resistant to p-fluorophenylalanine (PFP) and one resistant to 5-methyltryptophan (5-MT) are compared with wild type cells in their ability to absorb amino acids from the medium. One p-fluorophenylalanine-resistant cell line shows greatly reduced uptake of all amino acids so is resistant to growth inhibition by other amino acid analogs. The impaired absorption is noted with amino acids, amino acid analogs and shikimate, but not with cinnamate, salicylate, nicotine, glucose, 3-O-methylglucose and palmitate. The phenylalanine transport system of the PFP-resistant cell line and the wild type both have Km values of 90 micrograms, but have different Vmax values. Several analogs of phenylalanine and several neutral L-amino acids inihibt the phenylalanine transport system, while L-aspartic acid, L-arginine, D-phenylalanine or chlorogenic acid do not interfere with the L-phenylalanine uptake. The results indicate the presence of more than one transport system for amino acid uptake. The lessened uptake of all amino acids, the specificity of the uptake systems and the unchanged binding let us conclude that a pleiotropic mutation or that some inhibitor causes the reduced uptake of all amino acids by the PFP-resistant cell line.
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Claims 10
Claim · model-written summary
One PFP-resistant tobacco cell line had greatly reduced uptake of all amino acids.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- p-fluorophenylalanine-resistant cell linee0
- Outcome
- uptake of all amino acidse0
- Direction
- greatly reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One p-fluorophenylalanine-resistant cell line shows greatly reduced uptake of all amino acids”
t0“Tobacco Cell Lines”
Claim · model-written summary
That cell line resisted growth inhibition by other amino acid analogs.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- resistant to growth inhibition by other amino acid analogse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“so is resistant to growth inhibition by other amino acid analogs”
t0“Tobacco Cell Lines”
Claim · model-written summary
Absorption of amino acids, amino acid analogs and shikimate was impaired.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- impaired absorptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The impaired absorption is noted with amino acids, amino acid analogs and shikimate”
t0“Tobacco Cell Lines”
Claim · model-written summary
Absorption of cinnamate, salicylate, nicotine, glucose, 3-O-methylglucose and palmitate was not impaired.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- cinnamate, salicylate, nicotine, glucose, 3-O-methylglucose and palmitatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not with cinnamate, salicylate, nicotine, glucose, 3-O-methylglucose and palmitate”
t0“Tobacco Cell Lines”
Claim · model-written summary
The resistant line and wild type had phenylalanine transport Km values of 90 micrograms.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- PFP-resistant cell linee0
- Outcome
- Km valuese0
- Value
- 90 microgramse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phenylalanine transport system of the PFP-resistant cell line and the wild type both have Km values of 90 micrograms”
t0“Tobacco Cell Lines”
Claim · model-written summary
The resistant line and wild type differed in phenylalanine transport Vmax.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- different Vmax valuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but have different Vmax values”
t0“Tobacco Cell Lines”
Claim · model-written summary
Phenylalanine analogs and neutral L-amino acids inhibited phenylalanine transport.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Several analogs of phenylalanine and several neutral L-amino acidse0
- Outcome
- phenylalanine transport systeme0
- Direction
- inihibte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several analogs of phenylalanine and several neutral L-amino acids inihibt the phenylalanine transport system”
t0“Tobacco Cell Lines”
Claim · model-written summary
L-aspartic acid, L-arginine, D-phenylalanine and chlorogenic acid did not interfere with phenylalanine uptake.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- L-aspartic acid, L-arginine, D-phenylalanine or chlorogenic acide0
- Outcome
- L-phenylalanine uptakee0
- Direction
- do not interferee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while L-aspartic acid, L-arginine, D-phenylalanine or chlorogenic acid do not interfere with the L-phenylalanine uptake”
t0“Tobacco Cell Lines”
Claim · model-written summary
The results indicate multiple amino acid uptake transport systems.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- amino acid uptakee0
- Qualifier
- more than one transport systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicate the presence of more than one transport system for amino acid uptake”
t0“Tobacco Cell Lines”
Claim · model-written summary
A pleiotropic mutation or inhibitor was proposed to explain reduced amino acid uptake.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- reduced uptake of all amino acidse0
- Qualifier
- a pleiotropic mutation or that some inhibitore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a pleiotropic mutation or that some inhibitor causes the reduced uptake of all amino acids by the PFP-resistant cell line”
t0“Tobacco Cell Lines”
The same questions in other papers
phenylalanine · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.