Abstract
The effects of heavy metal cadmium (Cd), alone and in combination with zinc (Zn), on the root growth and activity of antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD) in Cucumis sativus L. hairy roots were studied. The purpose was to study the possibilities on using C. sativus hairy roots for phytoremediation of cadmium contamination. The results showed that less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture. At Cd concentrations above 15 mg/L hairy root growth was gradually inhibited with increasing Cd concentration. The roots formed were shorter with smaller lateral roots. Among all the Cd concentrations tested, except with 10 mg/L Cd, the soluble protein contents in the C. sativus hairy roots cultured with the other Cd concentrations decreased, but the POD and SOD activities increased gradually with time during the culture process. Further tests were conducted using a control culture containing 25 mg/L Zn alone. The addition of 1 mg/L Cd to the 25 mg/L Zn culture stimulated the growth of C. sativus hairy roots after 7-15 days of growth, compared with the control. At all other Cd concentrations the growth of C. sativus hairy roots was inhibited compared to the control. Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen. After 5 days culture with different concentrations of Cd + 25 mg/L Zn, the root biomass and the activity of POD and SOD were lower than in C. sativus hairy roots cultured without the addition of Zn. However, the soluble protein content was significantly higher when the culture contained 25 mg/L Zn. Our results suggested that C. sativus hairy roots have higher tolerance to heavy metal Cd but higher concentration of Cd inhibited the growth. Cd in combination with Zn would result in more serious Cd-induced growth inhibition.
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Claims 16
Claim · model-written summary
growth of C. sativus hairy roots was enhanced under less than 10 mg/L Cd.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- less than 10 mg/L Cde0
- Outcome
- growth of C. sativus hairy rootse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
root diameter was increased under less than 10 mg/L Cd.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- less than 10 mg/L Cde0
- Outcome
- root diametere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
hairy root growth was inhibited under Cd concentrations above 15 mg/L.
- Taxon
- C. sativus
Cucumis sativuse1
- Treatment
- Cd concentrations above 15 mg/Le0
- Outcome
- hairy root growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At Cd concentrations above 15 mg/L hairy root growth was gradually inhibited with increasing Cd concentration.”
e1“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
roots was shorter under nr.
- Taxon
- C. sativus
Cucumis sativuse1
- Outcome
- rootse0
- Direction
- shortere0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The roots formed were shorter with smaller lateral roots.”
e1“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
soluble protein contents was decreased under the other Cd concentrations.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- the other Cd concentrationse0
- Outcome
- soluble protein contentse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among all the Cd concentrations tested, except with 10 mg/L Cd, the soluble protein contents in the C. sativus hairy roots cultured with the other Cd concentrations decreased, but the POD and SOD activities increased gradually with time during the culture process.”
Claim · model-written summary
POD and SOD activities was increased under the other Cd concentrations.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- the other Cd concentrationse0
- Outcome
- POD and SOD activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among all the Cd concentrations tested, except with 10 mg/L Cd, the soluble protein contents in the C. sativus hairy roots cultured with the other Cd concentrations decreased, but the POD and SOD activities increased gradually with time during the culture process.”
Claim · model-written summary
growth of C. sativus hairy roots was stimulated under 1 mg/L Cd to the 25 mg/L Zn culture.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- 1 mg/L Cd to the 25 mg/L Zn culturee0
- Outcome
- growth of C. sativus hairy rootse0
- Direction
- stimulatede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of 1 mg/L Cd to the 25 mg/L Zn culture stimulated the growth of C. sativus hairy roots after 7-15 days of growth, compared with the control.”
Claim · model-written summary
growth of C. sativus hairy roots was inhibited under all other Cd concentrations.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- all other Cd concentrationse0
- Outcome
- growth of C. sativus hairy rootse0
- Direction
- inhibitede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At all other Cd concentrations the growth of C. sativus hairy roots was inhibited compared to the control.”
Claim · model-written summary
Growth inhibition was increased under increasing Cd concentration.
- Taxon
- C. sativus
Cucumis sativuse1
- Treatment
- increasing Cd concentratione0
- Outcome
- Growth inhibitione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen.”
e1“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
lateral roots was fewer, shorter and smaller under increasing Cd concentration.
- Taxon
- C. sativus
Cucumis sativuse1
- Treatment
- increasing Cd concentratione0
- Outcome
- lateral rootse0
- Direction
- fewer, shorter and smallere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen.”
e1“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
tips of which became swollen was na under increasing Cd concentration.
- Taxon
- C. sativus
Cucumis sativuse1
- Treatment
- increasing Cd concentratione0
- Outcome
- tips of which became swollene0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen.”
e1“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
root biomass was lower under different concentrations of Cd + 25 mg/L Zn.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- different concentrations of Cd + 25 mg/L Zne0
- Outcome
- root biomasse0
- Direction
- lowere0
- Comparator
- without the addition of Zne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 5 days culture with different concentrations of Cd + 25 mg/L Zn, the root biomass and the activity of POD and SOD were lower than in C. sativus hairy roots cultured without the addition of Zn.”
Claim · model-written summary
activity of POD and SOD was lower under different concentrations of Cd + 25 mg/L Zn.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- different concentrations of Cd + 25 mg/L Zne0
- Outcome
- activity of POD and SODe0
- Direction
- lowere0
- Comparator
- without the addition of Zne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 5 days culture with different concentrations of Cd + 25 mg/L Zn, the root biomass and the activity of POD and SOD were lower than in C. sativus hairy roots cultured without the addition of Zn.”
Claim · model-written summary
soluble protein content was higher under 25 mg/L Zn.
- Taxon
- C. sativus
Cucumis sativuse1
- Treatment
- 25 mg/L Zne0
- Outcome
- soluble protein contente0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, the soluble protein content was significantly higher when the culture contained 25 mg/L Zn.”
e1“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”
Claim · model-written summary
C. sativus hairy roots were interpreted as highly tolerant to Cd.
- Taxon
- C. sativus
Cucumis sativuse0
- Outcome
- higher tolerance to heavy metal Cde0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results suggested that C. sativus hairy roots have higher tolerance to heavy metal Cd but higher concentration of Cd inhibited the growth. Cd in combination with Zn would result in more serious Cd-induced growth inhibition.”
Claim · model-written summary
Cd with Zn was interpreted as causing more serious Cd-induced growth inhibition.
- Taxon
- C. sativus
Cucumis sativuse0
- Treatment
- Cd in combination with Zne0
- Outcome
- Cd-induced growth inhibitione0
- Direction
- more seriouse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results suggested that C. sativus hairy roots have higher tolerance to heavy metal Cd but higher concentration of Cd inhibited the growth. Cd in combination with Zn would result in more serious Cd-induced growth inhibition.”
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.