Paper · 2009 · PubMed

[Influences of heavy metal cadmium alone and in combination with zinc on the growth and activities of antioxidant enzymes of Cucumis sativus hairy roots].

Yan Zhang; Heping Shi; Eric Po; et al.

16
claims
22
verbatim quotes
2
citations
1
research areas
—
Not open access

Abstract

The effects of heavy metal cadmium (Cd), alone and in combination with zinc (Zn), on the root growth and activity of antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD) in Cucumis sativus L. hairy roots were studied. The purpose was to study the possibilities on using C. sativus hairy roots for phytoremediation of cadmium contamination. The results showed that less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture. At Cd concentrations above 15 mg/L hairy root growth was gradually inhibited with increasing Cd concentration. The roots formed were shorter with smaller lateral roots. Among all the Cd concentrations tested, except with 10 mg/L Cd, the soluble protein contents in the C. sativus hairy roots cultured with the other Cd concentrations decreased, but the POD and SOD activities increased gradually with time during the culture process. Further tests were conducted using a control culture containing 25 mg/L Zn alone. The addition of 1 mg/L Cd to the 25 mg/L Zn culture stimulated the growth of C. sativus hairy roots after 7-15 days of growth, compared with the control. At all other Cd concentrations the growth of C. sativus hairy roots was inhibited compared to the control. Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen. After 5 days culture with different concentrations of Cd + 25 mg/L Zn, the root biomass and the activity of POD and SOD were lower than in C. sativus hairy roots cultured without the addition of Zn. However, the soluble protein content was significantly higher when the culture contained 25 mg/L Zn. Our results suggested that C. sativus hairy roots have higher tolerance to heavy metal Cd but higher concentration of Cd inhibited the growth. Cd in combination with Zn would result in more serious Cd-induced growth inhibition.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 16

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

growth of C. sativus hairy roots was enhanced under less than 10 mg/L Cd.

Taxon
C. sativusCucumis sativuse0
Treatment
less than 10 mg/L Cde0
Outcome
growth of C. sativus hairy rootse0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

root diameter was increased under less than 10 mg/L Cd.

Taxon
C. sativusCucumis sativuse0
Treatment
less than 10 mg/L Cde0
Outcome
root diametere0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

hairy root growth was inhibited under Cd concentrations above 15 mg/L.

Taxon
C. sativusCucumis sativuse1
Treatment
Cd concentrations above 15 mg/Le0
Outcome
hairy root growthe0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At Cd concentrations above 15 mg/L hairy root growth was gradually inhibited with increasing Cd concentration.”

✓ located verbatim in the source · abstract, characters 509–619 · show in text ↑
e1

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

roots was shorter under nr.

Taxon
C. sativusCucumis sativuse1
Outcome
rootse0
Direction
shortere0

Not reportedTreatmentComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The roots formed were shorter with smaller lateral roots.”

✓ located verbatim in the source · abstract, characters 620–677 · show in text ↑
e1

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

soluble protein contents was decreased under the other Cd concentrations.

Taxon
C. sativusCucumis sativuse0
Treatment
the other Cd concentrationse0
Outcome
soluble protein contentse0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Among all the Cd concentrations tested, except with 10 mg/L Cd, the soluble protein contents in the C. sativus hairy roots cultured with the other Cd concentrations decreased, but the POD and SOD activities increased gradually with time during the culture process.”

✓ located verbatim in the source · abstract, characters 678–942 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

POD and SOD activities was increased under the other Cd concentrations.

Taxon
C. sativusCucumis sativuse0
Treatment
the other Cd concentrationse0
Outcome
POD and SOD activitiese0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Among all the Cd concentrations tested, except with 10 mg/L Cd, the soluble protein contents in the C. sativus hairy roots cultured with the other Cd concentrations decreased, but the POD and SOD activities increased gradually with time during the culture process.”

✓ located verbatim in the source · abstract, characters 678–942 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

growth of C. sativus hairy roots was stimulated under 1 mg/L Cd to the 25 mg/L Zn culture.

Taxon
C. sativusCucumis sativuse0
Treatment
1 mg/L Cd to the 25 mg/L Zn culturee0
Outcome
growth of C. sativus hairy rootse0
Direction
stimulatede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The addition of 1 mg/L Cd to the 25 mg/L Zn culture stimulated the growth of C. sativus hairy roots after 7-15 days of growth, compared with the control.”

✓ located verbatim in the source · abstract, characters 1025–1178 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

growth of C. sativus hairy roots was inhibited under all other Cd concentrations.

Taxon
C. sativusCucumis sativuse0
Treatment
all other Cd concentrationse0
Outcome
growth of C. sativus hairy rootse0
Direction
inhibitede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At all other Cd concentrations the growth of C. sativus hairy roots was inhibited compared to the control.”

✓ located verbatim in the source · abstract, characters 1179–1285 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Growth inhibition was increased under increasing Cd concentration.

Taxon
C. sativusCucumis sativuse1
Treatment
increasing Cd concentratione0
Outcome
Growth inhibitione0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen.”

✓ located verbatim in the source · abstract, characters 1286–1450 · show in text ↑
e1

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

lateral roots was fewer, shorter and smaller under increasing Cd concentration.

Taxon
C. sativusCucumis sativuse1
Treatment
increasing Cd concentratione0
Outcome
lateral rootse0
Direction
fewer, shorter and smallere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen.”

✓ located verbatim in the source · abstract, characters 1286–1450 · show in text ↑
e1

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

tips of which became swollen was na under increasing Cd concentration.

Taxon
C. sativusCucumis sativuse1
Treatment
increasing Cd concentratione0
Outcome
tips of which became swollene0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Growth inhibition increased with increasing Cd concentration, and the hairy roots formed fewer, shorter and smaller lateral roots, the tips of which became swollen.”

✓ located verbatim in the source · abstract, characters 1286–1450 · show in text ↑
e1

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

root biomass was lower under different concentrations of Cd + 25 mg/L Zn.

Taxon
C. sativusCucumis sativuse0
Treatment
different concentrations of Cd + 25 mg/L Zne0
Outcome
root biomasse0
Direction
lowere0
Comparator
without the addition of Zne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“After 5 days culture with different concentrations of Cd + 25 mg/L Zn, the root biomass and the activity of POD and SOD were lower than in C. sativus hairy roots cultured without the addition of Zn.”

✓ located verbatim in the source · abstract, characters 1451–1649 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

activity of POD and SOD was lower under different concentrations of Cd + 25 mg/L Zn.

Taxon
C. sativusCucumis sativuse0
Treatment
different concentrations of Cd + 25 mg/L Zne0
Outcome
activity of POD and SODe0
Direction
lowere0
Comparator
without the addition of Zne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“After 5 days culture with different concentrations of Cd + 25 mg/L Zn, the root biomass and the activity of POD and SOD were lower than in C. sativus hairy roots cultured without the addition of Zn.”

✓ located verbatim in the source · abstract, characters 1451–1649 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

soluble protein content was higher under 25 mg/L Zn.

Taxon
C. sativusCucumis sativuse1
Treatment
25 mg/L Zne0
Outcome
soluble protein contente0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“However, the soluble protein content was significantly higher when the culture contained 25 mg/L Zn.”

✓ located verbatim in the source · abstract, characters 1650–1750 · show in text ↑
e1

“less than 10 mg/L Cd enhanced the growth of C. sativus hairy roots and increased root diameter only in 5-15 days of root culture.”

✓ located verbatim in the source · abstract, characters 379–508 · show in text ↑
Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

C. sativus hairy roots were interpreted as highly tolerant to Cd.

Taxon
C. sativusCucumis sativuse0
Outcome
higher tolerance to heavy metal Cde0
Direction
highere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Our results suggested that C. sativus hairy roots have higher tolerance to heavy metal Cd but higher concentration of Cd inhibited the growth. Cd in combination with Zn would result in more serious Cd-induced growth inhibition.”

✓ located verbatim in the source · abstract, characters 1751–1978 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Cd with Zn was interpreted as causing more serious Cd-induced growth inhibition.

Taxon
C. sativusCucumis sativuse0
Treatment
Cd in combination with Zne0
Outcome
Cd-induced growth inhibitione0
Direction
more seriouse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Our results suggested that C. sativus hairy roots have higher tolerance to heavy metal Cd but higher concentration of Cd inhibited the growth. Cd in combination with Zn would result in more serious Cd-induced growth inhibition.”

✓ located verbatim in the source · abstract, characters 1751–1978 · show in text ↑

cadmium atom · growth · Cucumis sativus (cucumber)Results differ

18 papers: 2 positive, 16 negative or no effect

1 paper in the same direction as this paper · 16 in the opposite direction

  1. negative
  2. negative
  3. negative

14 papers more in this group

See all 18 papers →

zinc atom · physiology: antioxidant enzymes · Cucumis sativus (cucumber)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

2 in the opposite direction

  1. positive
  2. positive
See all 3 papers →

cadmium atom · physiology: antioxidant enzymes · Cucumis sativus (cucumber)Positive and negative results

9 papers: 5 positive, 4 negative or no effect

4 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. negative
  3. negative

5 papers more in this group

See all 9 papers →

zinc atom · growth · Cucumis sativus (cucumber)Positive and negative results

12 papers: 10 positive, 2 negative or no effect

9 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

8 papers more in this group

See all 12 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.