Abstract
A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation. 21 genotypes were established in culture from shoot apices (apical dome plus 2- to 4-leaf primordia) with a method previously developed for the Vitis hybrid Rougeon. However, this method proved to be inadequate for the establishment of the hybrid Seyval. Shoots grew on for 21 genotypes using a medium previously devised for the Vitis hybrid Remaily Seedless. Cultural conditions were inadequate for 4 of these genotypes. 10-h days resulted in best shoot production for 3 genotypes and 16-h days for 1. In general, shoot production was better or equal with short days than it was with long days. Shoots were multiplied from 3- to 4-node shoots (1.5 cm long) obtained in vitro. For the first 5 subcultures, 17 genotypes were multiplied on the medium used for first shoot production. Best results were obtained with Remaily Seedless which produced 13 shoots of at least 3 nodes, per subculture shoot after 2 months in culture. The 6th subculture employed the C2D salts which had previously been devised to improve shoot multiplication of Remaily Seedless. Yields were increased from 31 % to 350 % for all genotypes except V. labruscana Catawba which died in culture. For the rooting of subcultured shoots, a medium which had previously been developed for that purpose on Remaily Seedless was used successfully on 15 genotypes. Multiplication végétative de la vigne in vitro: Application des conditions de culture définies auparavant pour une sélection de génotypes La multiplication végétative in vitro a été réalisée pour une collection d'espèces, de cultivars et d'hybrides du genre Vitis. 21 génotypes furent mis en culture à partir d'apex de 2 à 4 ébauches foliaires, par une méthode que nous avions développé pour l'hybride Rougeon. Ces conditions de culture n'ont pas été favorables â la croissance de l'hybride Seyval. La production de pousses herbacées fut obtenue pour ces 21 génotypes sur un milieu nutritif que nous avions défini pour l'hybride Remaily Seedless. Pour 4 génotypes les conditions de cultures n'ont pas été favorables. L'influence de la photopériode sur la production de pousses fut étudiée également. Les journées de 10 h bénéficièrent 3 génotypes et les journées de 16 h un seul. En général, la production en jours courts s'est avérée meilleur qu'en jours longs. La multiplication des pousses fut éffectuée par repiquage de boutures terminales obtenues in vitro et comprenant 3 à 4 noeuds (1,5 cm). Pour les 5 premiers passages la multiplication de 17 génotypes fut continuée sur le milieu de culture utilisé pour la production des premières ·pousses. Les meilleurs résultats ont été obtenus pour Remaily Seedless. En 2 mois chaque bouture apicale de ce cultivar a produit en moyenne 13 pousses ayant au moins 3 noeuds. Le 6e repiquage a été fait sur un milieu, C2D, développé auparavant pour améliorer la multiplication de Remaily Seedless. La récolte fut augmentée ainsi de 31 % à 350 % selon le génotype sauf pour V. labruscana Catawba qui s'est nécrosé. Pour l'enracinement de boutures apicales obtenues en culture, un milieu défini à cet effet pour Remaily Seedless fut utilisé avec succès pour 15 génotypes.
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Claims 9
Claim · model-written summary
The method developed for the Vitis hybrid Rougeon was inadequate for establishing the hybrid Seyval in culture.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e2“this method proved to be inadequate for the establishment of the hybrid Seyval”
Claim · model-written summary
Twenty-one grapevine genotypes were established in culture from shoot apices.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e1“21 genotypes were established in culture from shoot apices (apical dome plus 2- to 4-leaf primordia) with a method previously developed for the Vitis hybrid Rougeon”
Claim · model-written summary
Cultural conditions were inadequate for 4 grapevine genotypes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e3“Cultural conditions were inadequate for 4 of these genotypes”
Claim · model-written summary
10-hour days gave the best shoot production for 3 grapevine genotypes.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e4“10-h days resulted in best shoot production for 3 genotypes and 16-h days for 1”
Claim · model-written summary
16-hour days gave the best shoot production for 1 grapevine genotype.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e4“10-h days resulted in best shoot production for 3 genotypes and 16-h days for 1”
Claim · model-written summary
Short days gave shoot production better than or equal to long days in grapevine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e5“In general, shoot production was better or equal with short days than it was with long days”
Claim · model-written summary
Remaily Seedless produced 13 shoots of at least 3 nodes per subculture shoot after 2 months in culture.
- Material
- Remaily Seedlesse6
- Outcome
- shoots of at least 3 nodese6
- Value
- 13 shootse6
- Qualifier
- after 2 months in culturee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e6“Best results were obtained with Remaily Seedless which produced 13 shoots of at least 3 nodes, per subculture shoot after 2 months in culture”
Claim · model-written summary
The C2D salts increased grapevine shoot multiplication yields from 31 % to 350 % for all genotypes except V. labruscana Catawba.
- Treatment
- C2D saltse7
- Outcome
- Yieldse6
- Direction
- increasede6
- Value
- 31 % to 350 %e6
- Qualifier
- except V. labruscana Catawba which died in culturee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e7“The 6th subculture employed the C2D salts which had previously been devised to improve shoot multiplication of Remaily Seedless”
e6“Yields were increased from 31 % to 350 % for all genotypes except V. labruscana Catawba which died in culture”
Claim · model-written summary
Rooting of subcultured grapevine shoots succeeded on 15 genotypes with a medium developed for Remaily Seedless.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A range of grapevine species, cultivars and hybrids was surveyed for their responses to in vitro micropropagation”
e1“For the rooting of subcultured shoots, a medium which had previously been developed for that purpose on Remaily Seedless was used successfully on 15 genotypes”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.