Abstract
Broccoli ( Brassica oleracea L. Botrytis group `Green Duke') seeds were cultured in vitro photoautotrophically (without sugar in the medium) or photomixotrophically (with sugar in the medium) for 3 weeks at 23 °C and 150 μmol·m -2 ·s -1 photosynthetic photon flux ( PPF ). Vessels were then stored at 5 °C under 1.6, 4.1, or 8.6 μmol·m -2 ·s -1 of white (400-800 nm), red (600-700 nm), or blue (400-500 nm) light. Concentrations of CO 2 inside the vessels were monitored until equilibrium was reached. Light compensation point was reached at 3.5 μmol·m -2 ·s -1 for photoautotrophic seedlings and at 6.5 μmol·m -2 ·s -1 for photomixotrophic seedlings. Therefore, in the long-term storage experiment, seedlings were stored for 4, 8, or 12 weeks at 5 °C in darkness or under 5 μmol·m -2 ·s -1 (average light compensation point) of white, red, or blue light. Illumination during storage was necessary to maintain dry mass, leaf area, and regrowth potentials of in vitro seedlings. All seedlings stored in darkness were of poor quality and died when transferred to the greenhouse. Red light during storage increased seedling dry mass and chlorophyll content and improved overall appearance, whereas blue light decreased chlorophyll content and increased stem elongation. The addition of 2% sucrose to media increased dry mass and leaf area and maintained overall seedling quality during illuminated storage. However, plantlets stored for more than 4 weeks did not survive poststorage greenhouse conditions, regardless of light treatment.
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Claims 9
Claim · model-written summary
The light compensation point was 3.5 μmol·m -2 ·s -1 for photoautotrophic seedlings.
- Taxon
- Broccoli
Brassica oleracea var. italicat0 - Material
- photoautotrophic seedlingse0
- Outcome
- Light compensation pointe0
- Value
- 3.5 μmol·m -2 ·s -1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light compensation point was reached at 3.5 μmol·m -2 ·s -1 for photoautotrophic seedlings”
t0“Broccoli Seedlings”
Claim · model-written summary
The light compensation point was 6.5 μmol·m -2 ·s -1 for photomixotrophic seedlings.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Outcome
- photomixotrophic seedlingse0
- Value
- 6.5 μmol·m -2 ·s -1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at 6.5 μmol·m -2 ·s -1 for photomixotrophic seedlings”
t0“Responses of Broccoli Seedlings to Light Quality”
Claim · model-written summary
Illumination during storage maintained seedling dry mass, leaf area and regrowth potential.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- Illumination during storagee0
- Outcome
- dry mass, leaf area, and regrowth potentialse0
- Direction
- maintaine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Illumination during storage was necessary to maintain dry mass, leaf area, and regrowth potentials of in vitro seedlings”
t0“Broccoli Seedlings”
Claim · model-written summary
Dark-stored seedlings were poor quality and died after transfer to the greenhouse.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- darknesse0
- Outcome
- poor quality and diede0
- Setting
- greenhousee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All seedlings stored in darkness were of poor quality and died when transferred to the greenhouse”
t0“Broccoli Seedlings”
Claim · model-written summary
Red light increased seedling dry mass and chlorophyll content and improved appearance.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- Red lighte0
- Outcome
- seedling dry mass and chlorophyll content and improved overall appearancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red light during storage increased seedling dry mass and chlorophyll content and improved overall appearance”
t0“Broccoli Seedlings”
Claim · model-written summary
Blue light decreased chlorophyll content.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- blue lighte0
- Outcome
- chlorophyll contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blue light decreased chlorophyll content”
t0“Broccoli Seedlings”
Claim · model-written summary
Blue light increased stem elongation.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- blue lighte0
- Outcome
- stem elongatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blue light decreased chlorophyll content and increased stem elongation”
t0“Broccoli Seedlings”
Claim · model-written summary
Adding 2% sucrose increased dry mass and leaf area and maintained seedling quality during illuminated storage.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- 2% sucrosee0
- Outcome
- dry mass and leaf area and maintained overall seedling qualitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of 2% sucrose to media increased dry mass and leaf area and maintained overall seedling quality during illuminated storage”
t0“Broccoli Seedlings”
Claim · model-written summary
Plantlets stored more than four weeks did not survive greenhouse conditions regardless of light treatment.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Outcome
- did not survivee0
- Qualifier
- more than 4 weekse0
- Setting
- greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantlets stored for more than 4 weeks did not survive poststorage greenhouse conditions, regardless of light treatment”
t0“Broccoli Seedlings”
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