Abstract
The phytohormone auxin plays critical roles in regulating myriads of plant growth and developmental processes. Microbe infection can disturb auxin signaling resulting in defects in these processes, but the underlying mechanisms are poorly understood. Auxin signaling begins with perception of auxin by a transient co-receptor complex consisting of an F-box transport inhibitor response 1/auxin signaling F-box (TIR1/AFB) protein and an auxin/indole-3-acetic acid (Aux/IAA) protein. Auxin binding to the co-receptor triggers ubiquitination and 26S proteasome degradation of the Aux/IAA proteins, leading to subsequent events, including expression of auxin-responsive genes. Here we report that Rice dwarf virus (RDV), a devastating pathogen of rice, causes disease symptoms including dwarfing, increased tiller number and short crown roots in infected rice as a result of reduced sensitivity to auxin signaling. The RDV capsid protein P2 binds OsIAA10, blocking the interaction between OsIAA10 and OsTIR1 and inhibiting 26S proteasome-mediated OsIAA10 degradation. Transgenic rice plants overexpressing wild-type or a dominant-negative (degradation-resistant) mutant of OsIAA10 phenocopy RDV symptoms are more susceptible to RDV infection; however, knockdown of OsIAA10 enhances the resistance of rice to RDV infection. Our findings reveal a previously unknown mechanism of viral protein reprogramming of a key step in auxin signaling initiation that enhances viral infection and pathogenesis.
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Claims 10
Claim · model-written summary
RDV infection caused dwarfing, increased tiller number and short crown roots in rice.
- Taxon
- rice
Oryza sativae0 - Interactor
- Rice dwarf virus (RDV)e0pathogen
- Outcome
- dwarfing, increased tiller number and short crown rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rice dwarf virus (RDV), a devastating pathogen of rice, causes disease symptoms including dwarfing, increased tiller number and short crown roots in infected rice”
Claim · model-written summary
RDV symptoms resulted from reduced sensitivity to auxin signalling.
- Taxon
- riceOryza sativae0
- Interactor
- Rice dwarf virus (RDV)e1pathogen
- Outcome
- disease symptoms including dwarfing, increased tiller number and short crown rootse0
- Qualifier
- reduced sensitivity to auxin signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“causes disease symptoms including dwarfing, increased tiller number and short crown roots in infected rice as a result of reduced sensitivity to auxin signaling”
e1“Rice dwarf virus (RDV), a devastating pathogen of rice”
Claim · model-written summary
RDV P2 bound rice OsIAA10.
- Taxon
- RiceOryza sativat0
- Interactor
- RDVe0pathogen
- Gene
- OsIAA10e0
- Outcome
- P2 binds OsIAA10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RDV capsid protein P2 binds OsIAA10”
t0“Rice OsIAA10 Protein”
Claim · model-written summary
P2 blocked the OsIAA10–OsTIR1 interaction.
- Taxon
- RiceOryza sativat0
- Interactor
- RDVe0pathogen
- Gene
- OsIAA10e0
- Outcome
- interaction between OsIAA10 and OsTIR1e0
- Qualifier
- blockinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RDV capsid protein P2 binds OsIAA10, blocking the interaction between OsIAA10 and OsTIR1”
t0“Rice OsIAA10 Protein”
Claim · model-written summary
P2 inhibited proteasome mediated OsIAA10 degradation.
- Taxon
- RiceOryza sativat0
- Interactor
- RDVe1pathogen
- Gene
- OsIAA10e0
- Outcome
- 26S proteasome-mediated OsIAA10 degradatione0
- Qualifier
- inhibitinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibiting 26S proteasome-mediated OsIAA10 degradation”
e1“The RDV capsid protein P2 binds OsIAA10”
t0“Rice OsIAA10 Protein”
Claim · model-written summary
Rice overexpressing wild type OsIAA10 phenocopied RDV symptoms.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing wild-typee0
- Interactor
- RDVe0pathogen
- Gene
- OsIAA10e0
- Outcome
- phenocopy RDV symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice plants overexpressing wild-type or a dominant-negative (degradation-resistant) mutant of OsIAA10 phenocopy RDV symptoms are more susceptible to RDV infection”
Claim · model-written summary
Rice expressing dominant negative OsIAA10 phenocopied RDV symptoms.
- Taxon
- riceOryza sativae0
- Treatment
- a dominant-negative (degradation-resistant) mutant of OsIAA10e0
- Interactor
- RDVe0pathogen
- Gene
- OsIAA10e0
- Outcome
- phenocopy RDV symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice plants overexpressing wild-type or a dominant-negative (degradation-resistant) mutant of OsIAA10 phenocopy RDV symptoms are more susceptible to RDV infection”
Claim · model-written summary
Rice overexpressing wild type OsIAA10 was more susceptible to RDV.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing wild-typee0
- Interactor
- RDVe0pathogen
- Gene
- OsIAA10e0
- Outcome
- susceptible to RDV infectione0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice plants overexpressing wild-type or a dominant-negative (degradation-resistant) mutant of OsIAA10 phenocopy RDV symptoms are more susceptible to RDV infection”
Claim · model-written summary
Rice expressing dominant negative OsIAA10 was more susceptible to RDV.
- Taxon
- riceOryza sativae0
- Treatment
- a dominant-negative (degradation-resistant) mutant of OsIAA10e0
- Interactor
- RDVe0pathogen
- Gene
- OsIAA10e0
- Outcome
- susceptible to RDV infectione0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice plants overexpressing wild-type or a dominant-negative (degradation-resistant) mutant of OsIAA10 phenocopy RDV symptoms are more susceptible to RDV infection”
Claim · model-written summary
OsIAA10 knockdown enhanced rice resistance to RDV.
- Taxon
- riceOryza sativae0
- Treatment
- knockdown of OsIAA10e0
- Interactor
- RDVe0pathogen
- Gene
- OsIAA10e0
- Outcome
- resistance of rice to RDV infectione0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockdown of OsIAA10 enhances the resistance of rice to RDV infection”
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loss of function · disease · Oryza sativa (rice)Positive and negative results
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