Abstract
Desiccation tolerance (DT) is a remarkable process that allows seeds in the dry state to remain viable for long periods of time that in some instances exceed 1,000 y. It has been postulated that seed DT evolved by rewiring the regulatory and signaling networks that controlled vegetative DT, which itself emerged as a crucial adaptive trait of early land plants. Understanding the networks that regulate seed desiccation tolerance in model plant systems would provide the tools to understand an evolutionary process that played a crucial role in the diversification of flowering plants. In this work, we used an integrated approach that included genomics, bioinformatics, metabolomics, and molecular genetics to identify and validate molecular networks that control the acquisition of DT in Arabidopsis seeds. Two DT-specific transcriptional subnetworks were identified related to storage of reserve compounds and cellular protection mechanisms that act downstream of the embryo development master regulators LEAFY COTYLEDON 1 and 2, FUSCA 3, and ABSCICIC ACID INSENSITIVE 3. Among the transcription factors identified as major nodes in the DT regulatory subnetworks, PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-intolerant mutant background to play an important role in seed DT. Additionally, we found that constitutive expression of PLATZ1 in WT plants confers partial DT in vegetative tissues.
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Claims 5
Claim · model-written summary
Two desiccation tolerance specific subnetworks were identified for reserve storage and cellular protection downstream of embryo regulators.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Gene
- LEAFY COTYLEDON 1 and 2e0
- Outcome
- DT-specific transcriptional subnetworkse0
- Value
- Twoe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Two DT-specific transcriptional subnetworks were identified related to storage of reserve compounds and cellular protection mechanisms that act downstream of the embryo development master regulators LEAFY COTYLEDON 1 and 2, FUSCA 3, and ABSCICIC ACID INSENSITIVE 3.”
Claim · model-written summary
PLATZ1 was confirmed as important for seed desiccation tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PLATZ1e0
- Outcome
- seed DTe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-intolerant mutant background to play an important role in seed DT.”
Claim · model-written summary
PLATZ2 was confirmed as important for seed desiccation tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PLATZ2e0
- Outcome
- seed DTe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-intolerant mutant background to play an important role in seed DT.”
Claim · model-written summary
AGL67 was confirmed as important for seed desiccation tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AGL67e0
- Outcome
- seed DTe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-intolerant mutant background to play an important role in seed DT.”
Claim · model-written summary
Constitutive PLATZ1 expression conferred partial desiccation tolerance to vegetative tissues.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- vegetative tissuese0
- Treatment
- constitutive expression of PLATZ1e0
- Gene
- PLATZ1e0
- Outcome
- partial DTe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“constitutive expression of PLATZ1 in WT plants confers partial DT in vegetative tissues.”
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