Paper · 2016 · Journal of Integrative Agriculture

Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

Wen CHENG; Jin-shan GAO; Xing-xing FENG; et al.

7
claims
14
verbatim quotes
13
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M2 population. Two of these mutants, Gmdwf1 (Glycine max dwarf 1) and Gmdwf2 (Glycine max dwarf 2), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Forty-four dwarf mutants were identified in a mutagenized soybean population.

Taxon
soybeanGlycine maxt0
Material
gamma rays mutagenized M2 populatione0
Outcome
dwarf mutants were identifiede0
Value
44e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“44 dwarf mutants were identified from a gamma rays mutagenized M2 population”

✓ located verbatim in the source · abstract, characters 222–298 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 76–83 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Gmdwf1 and Gmdwf2 dwarf phenotypes were recessively inherited.

Taxon
soybeanGlycine maxt0
Material
both mutantse0
Outcome
inherited in a recessive mannere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Genetic analysis showed that both mutants were inherited in a recessive manner”

✓ located verbatim in the source · abstract, characters 418–496 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 76–83 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The two mutants mapped to a 2.610-Mb region on chromosome 1.

Taxon
soybeanGlycine maxt0
Gene
chromosome 1e0
Outcome
mutated regions were delimitede0
Value
2.610-Mbe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping”

✓ located verbatim in the source · abstract, characters 501–597 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 76–83 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fine mapping found a shared 1.552 Mb deletion in a previously unreported locus.

Taxon
soybeanGlycine maxt0
Gene
novel locus sitee0
Outcome
common deletion regione0
Value
1.552 Mbe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously”

✓ located verbatim in the source · abstract, characters 638–790 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 76–83 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Gibberellin treatment did not rescue Gmdwf1 dwarfism.

Taxon
soybeanGlycine maxt0
Material
Gmdwf1e0
Treatment
gibberellin (GA)e0
Outcome
dwarfism of Gmdwf1e0
Direction
could not be rescuede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments”

✓ located verbatim in the source · abstract, characters 792–888 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 76–83 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Brassinolide treatment did not rescue Gmdwf1 dwarfism.

Taxon
soybeanGlycine maxt0
Material
Gmdwf1e0
Treatment
brassinolide (BR)e0
Outcome
dwarfism of Gmdwf1e0
Direction
could not be rescuede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments”

✓ located verbatim in the source · abstract, characters 792–888 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 76–83 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors infer that hormone biosynthesis is not deficient in Gmdwf1.

Taxon
soybeanGlycine maxt0
Material
Gmdwf1e0
Outcome
biosynthesis of these hormones was not deficiente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1”

✓ located verbatim in the source · abstract, characters 890–973 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 76–83 · show in text ↑

Gibberellic acid · growth · Glycine max (soybean)Results differ

35 papers: 30 positive, 5 negative or no effect

4 papers in the same direction as this paper · 30 in the opposite direction

  1. positive
  2. positive
  3. positive

31 papers more in this group

See all 35 papers →

brassinolide · growth · Glycine max (soybean)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

1 paper in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

class · growth · Glycine max (soybean)Positive and negative results

62 papers: 47 positive, 15 negative or no effect

14 papers in the same direction as this paper · 47 in the opposite direction

  1. positive
  2. positive
  3. positive

58 papers more in this group

See all 62 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.