Abstract
Fe deficiency causes significant losses to crop productivity and quality. To understand better the mechanisms of plant responses to Fe deficiency, we used an in vitro cotton ovule culture system. We found that Fe deficiency suppressed the development of ovules and fibers, and led to tissue browning. RNA-seq analysis showed that the myo-inositol and galacturonic acid pathways were activated and cytosolic APX (ascorbate peroxidase) was suppressed in Fe-deficient treated fibers, which increased ASC (ascorbate) concentrations to prevent tissue browning. Suppression of cytosolic APX by RNAi in cotton increased ASC contents and delayed tissue browning by maintaining ferric reduction activity under Fe-deficient conditions. Meanwhile, APX RNAi line also exhibited the activation of expression of iron-regulated transporter (IRT1) and ferric reductase-oxidase2 (FRO2) to adapt to Fe deficiency. ABA levels were significantly decreased in Fe-deficient treated ovules and fibers, while the upregulated expression of ABA biosynthesis genes and suppression of ABA degradation genes in Fe-deficient ovules slowed down the decreased of ABA in cytosolic APX suppressed lines to delay the tissue browning. Moreover, the application of ABA in Fe-deficient medium suppressed the development of tissue browning and completely restored the ferric reduction activity. In addition, ABA 8’-hydroxylase gene (GhABAH1) overexpressed cotton has a decreased level of ABA and shows more sensitivity to Fe deficiency. Based on the results, we speculate that ASC could improve the tolerance to Fe deficiency through activating Fe uptake and maintaining ABA levels in cotton ovules and fibers, which in turn reduces symptom formation.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 15
Claim · model-written summary
Fe deficiency suppressed the development of ovules and fibers in cotton.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- Fe deficiencye0
- Outcome
- development of ovules and fiberse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“Fe deficiency suppressed the development of ovules and fibers”
Claim · model-written summary
Fe deficiency led to tissue browning in cotton ovule culture.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Fe deficiencye0
- Outcome
- tissue browninge0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“Fe deficiency suppressed the development of ovules and fibers, and led to tissue browning”
Claim · model-written summary
RNA-seq showed that the myo-inositol and galacturonic acid pathways were activated in Fe-deficient treated cotton fibers.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- fiberse0
- Treatment
- Fe-deficiente0
- Outcome
- myo-inositol and galacturonic acid pathwayse0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“the myo-inositol and galacturonic acid pathways were activated and cytosolic APX (ascorbate peroxidase) was suppressed in Fe-deficient treated fibers”
Claim · model-written summary
RNA-seq showed that cytosolic APX was suppressed in Fe-deficient treated cotton fibers.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- fiberse0
- Treatment
- Fe-deficiente0
- Gene
- cytosolic APX (ascorbate peroxidase)e0
- Outcome
- cytosolic APX (ascorbate peroxidase)e0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“the myo-inositol and galacturonic acid pathways were activated and cytosolic APX (ascorbate peroxidase) was suppressed in Fe-deficient treated fibers”
Claim · model-written summary
The pathway activation and APX suppression increased ascorbate concentrations, which the authors say prevents tissue browning in Fe-deficient cotton fibers.
- Taxon
- cottonGossypium hirsutumt0
- Compound
- ASC (ascorbate)e0
- Outcome
- tissue browninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“which increased ASC (ascorbate) concentrations to prevent tissue browning”
Claim · model-written summary
Suppression of cytosolic APX by RNAi in cotton increased ascorbate contents.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Suppression of cytosolic APX by RNAie0
- Gene
- cytosolic APXe0
- Outcome
- ASC contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of cytosolic APX by RNAi in cotton increased ASC contents”
Claim · model-written summary
Suppression of cytosolic APX by RNAi in cotton delayed tissue browning by maintaining ferric reduction activity under Fe-deficient conditions.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Suppression of cytosolic APX by RNAie0
- Gene
- cytosolic APXe0
- Outcome
- tissue browninge0
- Direction
- delayede0
- Qualifier
- maintaining ferric reduction activity under Fe-deficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of cytosolic APX by RNAi in cotton increased ASC contents and delayed tissue browning by maintaining ferric reduction activity under Fe-deficient conditions”
Claim · model-written summary
The APX RNAi cotton line showed activation of expression of IRT1 and FRO2, adapting to Fe deficiency.
- Taxon
- cottonGossypium hirsutumt0
- Material
- APX RNAi linee0
- Gene
- iron-regulated transporter (IRT1) and ferric reductase-oxidase2 (FRO2)e0
- Outcome
- activation of expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“APX RNAi line also exhibited the activation of expression of iron-regulated transporter (IRT1) and ferric reductase-oxidase2 (FRO2)”
Claim · model-written summary
ABA levels were significantly decreased in Fe-deficient treated cotton ovules and fibers.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- ovules and fiberse0
- Treatment
- Fe-deficiente0
- Outcome
- ABA levelse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“ABA levels were significantly decreased in Fe-deficient treated ovules and fibers”
Claim · model-written summary
Upregulated ABA biosynthesis genes and suppressed ABA degradation genes in Fe-deficient ovules slowed the ABA decrease in cytosolic APX-suppressed lines, delaying tissue browning.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- ovulese0
- Gene
- ABA biosynthesis genese0
- Outcome
- tissue browninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“the upregulated expression of ABA biosynthesis genes and suppression of ABA degradation genes in Fe-deficient ovules slowed down the decreased of ABA in cytosolic APX suppressed lines to delay the tissue browning”
Claim · model-written summary
Applying ABA in Fe-deficient medium suppressed the development of tissue browning in cotton.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- application of ABA in Fe-deficient mediume0
- Outcome
- development of tissue browninge0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“the application of ABA in Fe-deficient medium suppressed the development of tissue browning and completely restored the ferric reduction activity”
Claim · model-written summary
Applying ABA in Fe-deficient medium completely restored the ferric reduction activity in cotton.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- application of ABA in Fe-deficient mediume0
- Outcome
- ferric reduction activitye0
- Direction
- completely restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton (Gossypium hirsutum)”
e0“the application of ABA in Fe-deficient medium suppressed the development of tissue browning and completely restored the ferric reduction activity”
Claim · model-written summary
GhABAH1-overexpressing cotton had a decreased level of ABA.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- overexpressede0
- Gene
- GhABAH1e0
- Outcome
- level of ABAe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhABAH1) overexpressed cotton has a decreased level of ABA and shows more sensitivity to Fe deficiency”
Claim · model-written summary
GhABAH1-overexpressing cotton showed more sensitivity to Fe deficiency.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- overexpressede0
- Gene
- GhABAH1e0
- Outcome
- sensitivity to Fe deficiencye0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhABAH1) overexpressed cotton has a decreased level of ABA and shows more sensitivity to Fe deficiency”
Claim · model-written summary
The authors speculate that ascorbate could improve tolerance to Fe deficiency in cotton ovules and fibers by activating Fe uptake and maintaining ABA levels.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- ovules and fiberse0
- Compound
- ASCe0
- Outcome
- tolerance to Fe deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we speculate that ASC could improve the tolerance to Fe deficiency through activating Fe uptake and maintaining ABA levels in cotton ovules and fibers”
The same questions in other papers
iron atom · growth · Gossypium hirsutum (cotton)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- no effect
- positive
- positive
3 papers more in this group
See all 7 papers →iron atom · disease · Gossypium hirsutum (cotton)Results agree
2 papers in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
(+)-abscisic acid · disease · Gossypium hirsutum (cotton)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
in vitro · disease · Gossypium hirsutum (cotton)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.