Abstract
Several authors report the synthesis of periclinal chimeras generated from graft unions of Solanaceous plants grown in the greenhouse. As this technique requires shoot organogenesis, in vitro conditions are necessary to adapt this technique to woody species. We now report several in vitro techniques necessary to mimic the in vivo graft chimera process. These include rootstock/scion preparation, micrografting and shoot organogenesis from graft unions. Zeatin and auxins have been helpful in preparing graftable material and for increasing the percentage successful grafts. A shorter exposure to organogenic medium containing thidiazuron resulted in greater percentage shoot regeneration from graft unions. Thorny/thornless Rubus and 'Liberty'/'Golden Delicious' or 'Gala' Malus (color) markers are being used to determine the percentage of these regenerants which are chimeral.
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Claims 2
Claim · model-written summary
Zeatin and auxins improved graftable material and graft success.
- Taxon
- Solanaceous plantst0
- Treatment
- Zeatin and auxinse0
- Outcome
- percentage successful graftse0
- Direction
- increasinge0
Not reportedComparator
Verbatim
e0“Zeatin and auxins have been helpful in preparing graftable material and for increasing the percentage successful grafts.”
t0“Solanaceous plants grown in the greenhouse”
Claim · model-written summary
Shorter thidiazuron medium exposure increased shoot regeneration from graft unions.
- Treatment
- shorter exposure to organogenic medium containing thidiazurone0
- Outcome
- percentage shoot regeneration from graft unionse0
- Direction
- greatere0
RubusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A shorter exposure to organogenic medium containing thidiazuron resulted in greater percentage shoot regeneration from graft unions.”
t0“Thorny/thornless Rubus”
The same questions in other papers
Thidiazuron · growth · Rubus (bramble)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →in vitro · growth · Rubus (bramble)Positive and negative results
12 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.