Abstract
An efficient regeneration system is described based on the use of several genotypes and combining different cytokinins in the regeneration process. Optimal regeneration efficiency can be obtained if the factors affecting regeneration are examined with special attention to the maintenance of the stock plants, the composition of the medium, and the pre-treatment. The maintenance of stock plants proved to be optimal if the plants were kept on modified LS medium supplemented with 0.125 mg L −1 6-benzylaminopurine (BAP) and 0.01 mg L −1 indole-3-butyric acid (IBA) in large vessels. Pre-treatment was found to increase the regeneration efficiency. Placing the leaves on to medium containing 1.5 mg L −1 BAP-riboside and 0.1 mg L −1 thidiazuron (TDZ) without wounding, and keeping them in the dark for 6 days gave the best results. The highest regeneration rate was observed on medium containing MS salts with B5 vitamins complemented with 20 g L −1 glucose, 3 mg L −1 BAP-riboside, 0.2 mg L −1 TDZ and 0.2 mg L −1 IBA. This system made it possible to achieve regeneration in each of the varieties examined, though to different extents.
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Claims 5
Claim · model-written summary
Stock plant maintenance was optimal on modified LS medium with BAP and IBA in large vessels.
- Treatment
- modified LS medium supplemented with 0.125 mg L −1 6-benzylaminopurine (BAP) and 0.01 mg L −1 indole-3-butyric acid (IBA)e0
- Outcome
- maintenance of stock plantse0
- Direction
- optimale0
- Setting
- large vesselse0
RubusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rubus species”
e0“The maintenance of stock plants proved to be optimal if the plants were kept on modified LS medium supplemented with 0.125 mg L −1 6-benzylaminopurine (BAP) and 0.01 mg L −1 indole-3-butyric acid (IBA) in large vessels.”
Claim · model-written summary
Pretreatment increased Rubus regeneration efficiency.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rubus species”
e0“Pre-treatment was found to increase the regeneration efficiency.”
Claim · model-written summary
Unwounded leaves on BAP-riboside and TDZ medium in darkness for six days gave the best regeneration results.
- Organ / stage
- leavese0
- Treatment
- medium containing 1.5 mg L −1 BAP-riboside and 0.1 mg L −1 thidiazuron (TDZ) without wounding, and keeping them in the dark for 6 dayse0
- Outcome
- best resultse0
- Direction
- beste0
- Qualifier
- without woundinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rubus species”
e0“Placing the leaves on to medium containing 1.5 mg L −1 BAP-riboside and 0.1 mg L −1 thidiazuron (TDZ) without wounding, and keeping them in the dark for 6 days gave the best results.”
Claim · model-written summary
The highest regeneration rate occurred on MS salts and B5 vitamins medium supplemented with glucose, BAP-riboside, TDZ and IBA.
- Treatment
- medium containing MS salts with B5 vitamins complemented with 20 g L −1 glucose, 3 mg L −1 BAP-riboside, 0.2 mg L −1 TDZ and 0.2 mg L −1 IBAe0
- Outcome
- regeneration ratee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rubus species”
e0“The highest regeneration rate was observed on medium containing MS salts with B5 vitamins complemented with 20 g L −1 glucose, 3 mg L −1 BAP-riboside, 0.2 mg L −1 TDZ and 0.2 mg L −1 IBA.”
Claim · model-written summary
The system regenerated every Rubus variety examined, although regeneration extent varied.
- Outcome
- regeneration in each of the varieties examinede0
- Value
- each of the varieties examinede0
- Qualifier
- though to different extentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rubus species”
e0“This system made it possible to achieve regeneration in each of the varieties examined, though to different extents.”
The same questions in other papers
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.