Abstract
We aimed to investigate the role of the plasma membrane-localized sucrose/H+ symporter gene (NtSUT1) in root elongation growth of tobacco seedlings (Nicotiana tabacum L. cv. Samsun NN) under aluminum (Al) stress, by comparative study between wild-type (WT) tobacco and constructed over-expression (OX) and suppression (RNAi) lines of NtSUT1, in which the overexpression or suppression of NtSUT1 was controlled by the cauliflower mosaic virus 35S promoter. Seedlings of each line were grown in a nutrient medium (pH 4.5) without or with aluminum chloride (AlCl3). The roots were analyzed to determine the degree of root elongation and the transcript levels of NtSUT1, soluble sugar content, and cell survival at the root apex. The transcript level of NtSUT1 at the root apex was negatively affected by Al. Compared with WT, OX lines and RNAi lines had higher and lower contents of soluble sugars at the root apex, respectively. Under a 16-h light/8-h dark photoperiod and in the absence of Al, the root elongation rate was similar in OX and WT, but was significantly decreased in RNAi. In the presence of Al, the degree of Al-induced inhibition of root elongation was lower in OX and higher in RNAi than in WT. At the root apex, the initiation of cell death during a 24-h Al treatment was decelerated in OX and accelerated in RNAi, compared with that in WT. The number of live cells remaining at the root apex after the 24-h Al treatment was higher in OX and lower in RNAi than in WT. In the dark, root growth was supported by sucrose supplied in the medium as a substitute for photoassimilate. Under these conditions in the absence or presence of Al, there were strong positive correlations between the transcript level of NtSUT1, the soluble sugar content at the root apex and the root elongation rate. Together, these results indicate that NtSUT1 at the root apex primarily supports sucrose uptake via the apoplastic pathway, and that sucrose positively affects root elongation. We conclude that overexpression of NtSUT1 at the root apex increases the soluble sugar content, resulting in greater cell survival and root elongation under Al stress, leading to the Al-tolerance phenotype.
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Claims 16
Claim · model-written summary
Al negatively affected NtSUT1 transcript level at the root apex.
- Taxon
- tobacco
Nicotiana tabacumt0 - Organ / stage
- root apexe0
- Treatment
- Ale0
- Gene
- NtSUT1e0
- Outcome
- transcript level of NtSUT1e0
- Direction
- negatively affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of NtSUT1 at the root apex was negatively affected by Al”
t0“tobacco”
Claim · model-written summary
OX lines had higher soluble sugar content at the root apex than WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- root apexe0
- Treatment
- OX linese0
- Outcome
- contents of soluble sugarse0
- Direction
- highere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with WT, OX lines and RNAi lines had higher and lower contents of soluble sugars at the root apex, respectively”
t0“tobacco”
Claim · model-written summary
RNAi lines had lower soluble sugar content at the root apex than WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- root apexe0
- Treatment
- RNAi linese0
- Outcome
- contents of soluble sugarse0
- Direction
- lowere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with WT, OX lines and RNAi lines had higher and lower contents of soluble sugars at the root apex, respectively”
t0“tobacco”
Claim · model-written summary
Without Al, OX and WT had similar root elongation rates.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- OXe0
- Outcome
- root elongation ratee0
- Direction
- similare0
- Qualifier
- absence of Ale0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the absence of Al, the root elongation rate was similar in OX and WT”
t0“tobacco”
Claim · model-written summary
Without Al, RNAi significantly decreased root elongation relative to WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- RNAie0
- Outcome
- root elongation ratee0
- Direction
- decreasede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the root elongation rate was similar in OX and WT, but was significantly decreased in RNAi”
t0“tobacco”
Claim · model-written summary
With Al, OX had lower Al-induced inhibition of root elongation than WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- OXe0
- Outcome
- degree of Al-induced inhibition of root elongatione0
- Direction
- lowere0
- Qualifier
- presence of Ale0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of Al, the degree of Al-induced inhibition of root elongation was lower in OX and higher in RNAi than in WT”
t0“tobacco”
Claim · model-written summary
With Al, RNAi had higher Al-induced inhibition of root elongation than WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- RNAie0
- Outcome
- degree of Al-induced inhibition of root elongatione0
- Direction
- highere0
- Qualifier
- presence of Ale0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of Al, the degree of Al-induced inhibition of root elongation was lower in OX and higher in RNAi than in WT”
t0“tobacco”
Claim · model-written summary
OX decelerated cell-death initiation during 24-hour Al treatment compared with WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- OXe0
- Outcome
- initiation of cell deathe0
- Direction
- deceleratede0
- Qualifier
- 24-h Al treatmente0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the initiation of cell death during a 24-h Al treatment was decelerated in OX and accelerated in RNAi, compared with that in WT”
t0“tobacco”
Claim · model-written summary
RNAi accelerated cell-death initiation during 24-hour Al treatment compared with WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- RNAie0
- Outcome
- initiation of cell deathe0
- Direction
- acceleratede0
- Qualifier
- 24-h Al treatmente0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the initiation of cell death during a 24-h Al treatment was decelerated in OX and accelerated in RNAi, compared with that in WT”
t0“tobacco”
Claim · model-written summary
After Al treatment, OX retained more live root-apex cells than WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- OXe0
- Outcome
- number of live cells remaining at the root apexe0
- Direction
- highere0
- Qualifier
- 24-h Al treatmente0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of live cells remaining at the root apex after the 24-h Al treatment was higher in OX and lower in RNAi than in WT”
t0“tobacco”
Claim · model-written summary
After Al treatment, RNAi retained fewer live root-apex cells than WT.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- RNAie0
- Outcome
- number of live cells remaining at the root apexe0
- Direction
- lowere0
- Qualifier
- 24-h Al treatmente0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of live cells remaining at the root apex after the 24-h Al treatment was higher in OX and lower in RNAi than in WT”
t0“tobacco”
Claim · model-written summary
Supplied sucrose supported root growth in darkness.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- sucrose supplied in the mediume0
- Outcome
- root growthe0
- Direction
- supportede0
- Qualifier
- In the darke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the dark, root growth was supported by sucrose supplied in the medium as a substitute for photoassimilate”
t0“tobacco”
Claim · model-written summary
NtSUT1 transcript level, root-apex sugar content and root elongation were strongly positively correlated in darkness with or without Al.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- root apexe0
- Gene
- NtSUT1e0
- Outcome
- root elongation ratee0
- Direction
- positivee0
- Qualifier
- absence or presence of Ale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the absence or presence of Al, there were strong positive correlations between the transcript level of NtSUT1, the soluble sugar content at the root apex and the root elongation rate”
t0“tobacco”
Claim · model-written summary
Authors infer that root-apex NtSUT1 primarily supports apoplastic sucrose uptake.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtSUT1e0
- Outcome
- sucrose uptakee0
- Qualifier
- apoplastic pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtSUT1 at the root apex primarily supports sucrose uptake via the apoplastic pathway”
t0“tobacco”
Claim · model-written summary
Authors infer that sucrose positively affects root elongation.
- Taxon
- tobaccoNicotiana tabacumt0
- Compound
- sucrosee0
- Outcome
- root elongatione0
- Direction
- positively affectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose positively affects root elongation”
t0“tobacco”
Claim · model-written summary
Authors conclude that NtSUT1 overexpression increases root-apex soluble sugar content.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- overexpression of NtSUT1e0
- Outcome
- soluble sugar contente0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of NtSUT1 at the root apex increases the soluble sugar content”
t0“tobacco”
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See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.