Abstract
Reactive oxygen species (ROS) produced by the NADPH oxidase, respiratory burst oxidase homolog (RBOH), trigger signal transduction in diverse biological processes in plants. However, the functions of RBOH homologs in rice (Oryza sativa) and other gramineous plants are poorly understood. Ethylene induces the formation of lysigenous aerenchyma, which consists of internal gas spaces created by programmed cell death of cortical cells, in roots of gramineous plants under oxygen-deficient conditions. Here, we report that, in rice, one RBOH isoform (RBOHH) has a role in ethylene-induced aerenchyma formation in roots. Induction of RBOHH expression under oxygen-deficient conditions was greater in cortical cells than in cells of other root tissues. In addition, genes encoding group I calcium-dependent protein kinases (CDPK5 and CDPK13) were strongly expressed in root cortical cells. Coexpression of RBOHH with CDPK5 or CDPK13 induced ROS production in Nicotiana benthamiana leaves. Inhibitors of RBOH activity or cytosolic calcium influx suppressed ethylene-induced aerenchyma formation. Moreover, knockout of RBOHH by CRISPR/Cas9 reduced ROS accumulation and inducible aerenchyma formation in rice roots. These results suggest that RBOHH-mediated ROS production, which is stimulated by CDPK5 and/or CDPK13, is essential for ethylene-induced aerenchyma formation in rice roots under oxygen-deficient conditions.
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Claims 10
Claim · model-written summary
RBOHH was implicated in ethylene-induced rice root aerenchyma formation.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- rootse0
- Gene
- RBOHHe0
- Outcome
- ethylene-induced aerenchyma formatione0
- Qualifier
- has a rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice, one RBOH isoform (RBOHH) has a role in ethylene-induced aerenchyma formation in roots”
Claim · model-written summary
Oxygen deficiency induced stronger RBOHH expression in cortical cells than in other root tissues.
- Taxon
- riceOryza sativae0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Induction of RBOHH expression under oxygen-deficient conditions was greater in cortical cells than in cells of other root tissues”
t0“Rice”
Claim · model-written summary
CDPK5 and CDPK13 genes were strongly expressed in root cortical cells.
- Taxon
- riceOryza sativae0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes encoding group I calcium-dependent protein kinases (CDPK5 and CDPK13) were strongly expressed in root cortical cells”
t0“Rice”
Claim · model-written summary
Coexpressing RBOHH with CDPK5 induced ROS production in Nicotiana benthamiana leaves.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Coexpression of RBOHH with CDPK5e0
- Gene
- RBOHH with CDPK5e0
- Outcome
- ROS productione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coexpression of RBOHH with CDPK5 or CDPK13 induced ROS production in Nicotiana benthamiana leaves”
Claim · model-written summary
Coexpressing RBOHH with CDPK13 induced ROS production in Nicotiana benthamiana leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- Coexpression of RBOHH with CDPK5 or CDPK13e0
- Gene
- CDPK13e0
- Outcome
- ROS productione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coexpression of RBOHH with CDPK5 or CDPK13 induced ROS production in Nicotiana benthamiana leaves”
Claim · model-written summary
RBOH activity inhibitors suppressed ethylene-induced aerenchyma formation.
- Taxon
- riceOryza sativae0t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibitors of RBOH activity or cytosolic calcium influx suppressed ethylene-induced aerenchyma formation”
t0“Rice”
Claim · model-written summary
Cytosolic calcium influx inhibitors suppressed ethylene-induced aerenchyma formation.
- Taxon
- riceOryza sativae0t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibitors of RBOH activity or cytosolic calcium influx suppressed ethylene-induced aerenchyma formation”
t0“Rice”
Claim · model-written summary
RBOHH knockout reduced ROS accumulation in rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Treatment
- knockout of RBOHH by CRISPR/Cas9e0
- Gene
- RBOHHe0
- Outcome
- ROS accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout of RBOHH by CRISPR/Cas9 reduced ROS accumulation and inducible aerenchyma formation in rice roots”
Claim · model-written summary
RBOHH knockout reduced inducible aerenchyma formation in rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Treatment
- knockout of RBOHH by CRISPR/Cas9e0
- Gene
- RBOHHe0
- Outcome
- inducible aerenchyma formatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout of RBOHH by CRISPR/Cas9 reduced ROS accumulation and inducible aerenchyma formation in rice roots”
Claim · model-written summary
The authors infer that CDPK5- or CDPK13-stimulated RBOHH ROS is essential for ethylene-induced root aerenchyma under oxygen deficiency.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Gene
- RBOHHe0
- Outcome
- ethylene-induced aerenchyma formatione0
- Qualifier
- RBOHH-mediated ROS productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RBOHH-mediated ROS production, which is stimulated by CDPK5 and/or CDPK13, is essential for ethylene-induced aerenchyma formation in rice roots under oxygen-deficient conditions”
The same questions in other papers
calcium atom · physiology: hormones · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.