Abstract
Domain of Unknown Function 266 (DUF266) is a plant-specific domain. DUF266-containing proteins (DUF266 proteins) have been categorized as ‘not classified glycosyltransferases (GTnc)’ due to amino acid similarity with GTs. However, little is known about the function of DUF266 proteins. Phylogenetic analysis revealed that DUF266 proteins are only present in the land plants including moss and lycophyte. We report the functional characterization of one member of DUF266 proteins in Populus, PdDUF266A. PdDUF266A was ubiquitously expressed with high abundance in the xylem. In Populus transgenic plants overexpressing PdDUF266A ( OXPdDUF266A ), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type. Degree of polymerization of cellulose in OXPdDUF266A transgenic plants was also higher, whereas cellulose crystallinity index remained unchanged. Gene expression analysis indicated that cellulose biosynthesis-related genes such as CESA and SUSY were upregulated in mature leaf and xylem of OXPdDUF266A transgenic plants. Moreover, PdDUF266A overexpression resulted in an increase of biomass production. Their glucose contents and biomass phenotypes were further validated via heterologous expression of PdDUF266A in Arabidopsis. Results from saccharification treatment demonstrated that the rate of sugar release was increased by approximately 38% in the OXPdDUF266A transgenic plants. These results suggest that the overexpression of PdDUF266A can increase cellulose content, reduce recalcitrance, and enhance biomass production, and that PdDUF266A is a promising target for genetic manipulation for biofuel production.
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Claims 10
Claim · model-written summary
DUF266 proteins occur in land plants including moss and lycophyte.
- Taxon
- land plantsEmbryophytae0
- Gene
- DUF266 proteinse0
- Outcome
- only presente0
Verbatim
e0“DUF266 proteins are only present in the land plants including moss and lycophyte”
Claim · model-written summary
PdDUF266A was broadly expressed, especially in xylem.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PdDUF266A was ubiquitously expressed with high abundance in the xylem”
t0“Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus”
Claim · model-written summary
PdDUF266A overexpression raised glucose and cellulose contents.
- Treatment
- overexpressing PdDUF266Ae0
- Gene
- PdDUF266Ae0
- Outcome
- glucose and cellulose contentse0
- Direction
- highere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus”
e0“In Populus transgenic plants overexpressing PdDUF266A ( OXPdDUF266A ), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type”
Claim · model-written summary
PdDUF266A overexpression lowered lignin content.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Populus transgenic plants overexpressing PdDUF266A ( OXPdDUF266A ), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type”
Claim · model-written summary
Cellulose degree of polymerization was higher in transgenic Populus.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus”
e0“Degree of polymerization of cellulose in OXPdDUF266A transgenic plants was also higher”
Claim · model-written summary
Cellulose crystallinity index was unchanged.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus”
e0“cellulose crystallinity index remained unchanged”
t1“Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus”
Claim · model-written summary
CESA and SUSY genes were upregulated in mature leaf and xylem.
- Organ / stage
- mature leaf and xyleme0
- Treatment
- OXPdDUF266Ae0
- Gene
- CESA and SUSYe0
- Outcome
- upregulatede0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus”
e0“cellulose biosynthesis-related genes such as CESA and SUSY were upregulated in mature leaf and xylem of OXPdDUF266A transgenic plants”
Claim · model-written summary
PdDUF266A overexpression increased biomass production.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus”
e0“PdDUF266A overexpression resulted in an increase of biomass production”
Claim · model-written summary
Heterologous expression in Arabidopsis validated glucose and biomass phenotypes.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- PdDUF266Ae0
- Outcome
- glucose contents and biomass phenotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their glucose contents and biomass phenotypes were further validated via heterologous expression of PdDUF266A in Arabidopsis”
Claim · model-written summary
Sugar release rate increased by about 38% in transgenic plants.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Populus”
e0“the rate of sugar release was increased by approximately 38% in the OXPdDUF266A transgenic plants”
The same questions in other papers
overexpression · molecular: metabolites · Populus (poplar)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.