Abstract
The rice (Oryza sativa) OsCYP714D1 gene (also known as EUI) encodes a cytochrome P450 monooxygenase which functions as a gibberellin (GA)-deactivating enzyme, catalysing 16α, 17-epoxidation of non-13-hydroxylated GAs. To understand whether it would also reduce the production of active GAs and depress the growth rate in transgenic trees, we constitutively expressed OsCYP714D1 in the aspen hybrid clone Populus alba×P. berolinensis. Unexpectedly, ectopic expression of OsCYP714D1 in aspen positively regulated the biosynthesis of GAs, including the active GA1 and GA4, leading to promotion of the growth rate and biomass production in transgenic plants. Transgenic lines which showed significant expression of the introduced OsCYP714D1 gene accumulated a higher GA level and produced more numerous and longer xylem fibres than did the wild-type plants. Quantitative real-time PCR indicated that transcription of most homologous PtCYP714 genes was suppressed in these transgenic lines. Therefore, the promoted GA and biomass production in transgenic trees constitutively expressing OsCYP714D1 is probably attributed to the down-regulated expression of the native PtCYP714 homologues involved in the GA biosynthesis pathway, although their precise functions are yet to be further elucidated.
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Claims 6
Claim · model-written summary
Ectopic expression of OsCYP714D1 in aspen positively regulated the biosynthesis of gibberellins, including active GA1 and GA4.
- Material
- aspen hybrid clone Populus alba×P. berolinensise0
- Treatment
- ectopic expression of OsCYP714D1e1
- Outcome
- biosynthesis of GAs, including the active GA1 and GA4e1
- Direction
- positively regulatede1
PopulusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice CYP714D1 gene into Populus inhibits expression of its homologous genes”
e0“constitutively expressed OsCYP714D1 in the aspen hybrid clone Populus alba×P. berolinensis”
e1“ectopic expression of OsCYP714D1 in aspen positively regulated the biosynthesis of GAs, including the active GA1 and GA4, leading to promotion of the growth rate and biomass production in transgenic plants”
Claim · model-written summary
Ectopic expression of OsCYP714D1 promoted growth rate and biomass production in transgenic poplar.
- Treatment
- ectopic expression of OsCYP714D1e1
- Outcome
- growth rate and biomass productione1
- Direction
- promotione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice CYP714D1 gene into Populus inhibits expression of its homologous genes”
e1“ectopic expression of OsCYP714D1 in aspen positively regulated the biosynthesis of GAs, including the active GA1 and GA4, leading to promotion of the growth rate and biomass production in transgenic plants”
Claim · model-written summary
Transgenic lines expressing the introduced OsCYP714D1 gene had a higher GA level than wild-type plants.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice CYP714D1 gene into Populus inhibits expression of its homologous genes”
e2“Transgenic lines which showed significant expression of the introduced OsCYP714D1 gene accumulated a higher GA level and produced more numerous and longer xylem fibres than did the wild-type plants”
Claim · model-written summary
Transgenic lines expressing OsCYP714D1 produced more numerous and longer xylem fibres than wild-type plants.
- Organ / stage
- xyleme2
- Treatment
- introduced OsCYP714D1 genee2
- Outcome
- xylem fibrese2
- Direction
- more numerous and longere2
- Comparator
- wild-type plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice CYP714D1 gene into Populus inhibits expression of its homologous genes”
e2“Transgenic lines which showed significant expression of the introduced OsCYP714D1 gene accumulated a higher GA level and produced more numerous and longer xylem fibres than did the wild-type plants”
Claim · model-written summary
Transcription of most homologous PtCYP714 genes was suppressed in OsCYP714D1 transgenic poplar lines.
- Treatment
- OsCYP714D1e0
- Gene
- PtCYP714 genese3
- Outcome
- transcription of most homologous PtCYP714 genese3
- Direction
- suppressede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice CYP714D1 gene into Populus inhibits expression of its homologous genes”
e0“constitutively expressed OsCYP714D1 in the aspen hybrid clone Populus alba×P. berolinensis”
e3“Quantitative real-time PCR indicated that transcription of most homologous PtCYP714 genes was suppressed in these transgenic lines”
Claim · model-written summary
Promoted GA and biomass production in OsCYP714D1 transgenic trees is probably attributed to down-regulated native PtCYP714 homologues.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice CYP714D1 gene into Populus inhibits expression of its homologous genes”
e4“the promoted GA and biomass production in transgenic trees constitutively expressing OsCYP714D1 is probably attributed to the down-regulated expression of the native PtCYP714 homologues involved in the GA biosynthesis pathway”
The same questions in other papers
overexpression · molecular: metabolites · Populus (poplar)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.