Abstract
The objective of the present study is to develop callus induction and plant regeneration; an experiment was conducted to investigate the effect of growth regulators on callus induction in rice mature embryo culture. In this experiment, callus was initiated from the mature seed scutellumof two Malaysian indica rice, MRQ74 and MR269. Callus was induced using different concentrations of 2, 4-D and BAP on MS medium under dark condition or under 16/8 h photoperiod conditions. For complete plant regeneration, the calli of both varieties were planted for shoot regeneration on MS media supplemented with different concentrations of BAP and NAA, for root regeneration on IBA. The results revealed that the maximum callus inductions in MRQ74 and MR269 were observed in the MS medium containing 3 mg/L 2, 4-D with 0.1 mg/L BAP under dark conditions. Furthermore, our protocol uses mature seeds as the explants in both rice varieties in terms of callus induction. Additionally, green plant production of plants was also observed in all of the media tested for shoot regeneration and MS media supplemented with 3 mg/L of BAP and 0.1 mg/L of NAA was found to be responsible for the recovery of more highly regenerated shoots. Effective root induction (100%) was achieved in a medium containing 1mg/LIBA. The described protocol can be useful in the establishment of an in vitro system for rice that can be a major technique for a genetic transformation system and crop improvement.
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Claims 5
Claim · model-written summary
Both rice varieties had maximum callus induction on MS medium with 3 mg/L 2, 4-D and 0.1 mg/L BAP in darkness.
- Taxon
- Oryza sativa L.
Oryza sativat0 - Material
- MRQ74 and MR269e0
- Treatment
- MS medium containing 3 mg/L 2, 4-D with 0.1 mg/L BAP under dark conditionse0
- Outcome
- callus inductionse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L. var. MRQ74 and MR269”
e0“the maximum callus inductions in MRQ74 and MR269 were observed in the MS medium containing 3 mg/L 2, 4-D with 0.1 mg/L BAP under dark conditions”
Claim · model-written summary
Green plants were produced in every medium tested for shoot regeneration.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- green plant productione0
- Qualifier
- all of the media tested for shoot regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L. var. MRQ74 and MR269”
e0“green plant production of plants was also observed in all of the media tested for shoot regeneration”
Claim · model-written summary
MS medium with 3 mg/L BAP and 0.1 mg/L NAA yielded more regenerated shoots.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- MS media supplemented with 3 mg/L of BAP and 0.1 mg/L of NAAe0
- Outcome
- regenerated shootse0
- Direction
- more highlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L. var. MRQ74 and MR269”
e0“MS media supplemented with 3 mg/L of BAP and 0.1 mg/L of NAA was found to be responsible for the recovery of more highly regenerated shoots”
Claim · model-written summary
Medium containing 1mg/LIBA achieved 100% root induction.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- medium containing 1mg/LIBAe0
- Outcome
- root inductione0
- Direction
- Effectivee0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L. var. MRQ74 and MR269”
e0“Effective root induction (100%) was achieved in a medium containing 1mg/LIBA”
Claim · model-written summary
The authors present the mature seed protocol as useful for establishing an in vitro rice system for transformation and crop improvement.
- Taxon
- riceOryza sativae0
- Outcome
- establishment of an in vitro system for ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L. var. MRQ74 and MR269”
e0“The described protocol can be useful in the establishment of an in vitro system for rice that can be a major technique for a genetic transformation system and crop improvement”
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