Abstract
Arsenic (As) contamination in rice leads to yield decline and causes carcinogenic risk to human health. Although the role of nitric oxide (NO) in reducing As toxicity is known, NO-mediated genetic modulation in the plant during arsenic toxicity has not yet been established. We analyzed the key components of NO metabolism and the correlations between NO interaction and arsenic stress using rice as a relevant model plant. Illumina sequencing was used to investigate the NO-mediated genome-wide temporal transcriptomic modulation in rice root upon AsIII exposure during 12 days (d) of the growth period. Sodium nitroprusside (SNP) was used as NO donor. SNP supplementation resulted in marked decrease in ROS, cell death and As accumulation during AsIII stress. NO was found to modulate metal transporters particularly NIP, NRAMP, ABC and iron transporters, stress related genes such as CytP450, GSTs, GRXs, TFs, amino acid, hormone(s), signaling and secondary metabolism genes involved in As detoxification. We detected NO-mediated change in jasmonic acid (JA) content during AsIII stress. The study infers that NO reduces AsIII toxicity through modulating regulatory networks involved in As detoxification and JA biosynthesis.
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Claims 8
Claim · model-written summary
SNP supplementation markedly decreased ROS during AsIII stress in rice.
- Taxon
- rice
Oryza sativae1 - Treatment
- SNP supplementatione0
- Outcome
- ROSe0
- Direction
- decreasee0
- Qualifier
- during AsIII stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNP supplementation resulted in marked decrease in ROS, cell death and As accumulation during AsIII stress”
e1“rice root upon AsIII exposure”
Claim · model-written summary
SNP supplementation markedly decreased rice cell death during AsIII stress.
- Taxon
- riceOryza sativae1
- Treatment
- SNP supplementatione0
- Outcome
- cell deathe0
- Direction
- decreasee0
- Qualifier
- during AsIII stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNP supplementation resulted in marked decrease in ROS, cell death and As accumulation during AsIII stress”
e1“rice root upon AsIII exposure”
Claim · model-written summary
SNP supplementation markedly decreased arsenic accumulation in rice during AsIII stress.
- Taxon
- riceOryza sativae1
- Treatment
- SNP supplementatione0
- Outcome
- As accumulatione0
- Direction
- decreasee0
- Qualifier
- during AsIII stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNP supplementation resulted in marked decrease in ROS, cell death and As accumulation during AsIII stress”
e1“rice root upon AsIII exposure”
Claim · model-written summary
NO modulated rice NIP, NRAMP, ABC and iron transporter genes during arsenic stress.
- Taxon
- riceOryza sativae1
- Treatment
- NOe0
- Gene
- NIP, NRAMP, ABC and iron transporterse0
- Outcome
- metal transporterse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO was found to modulate metal transporters particularly NIP, NRAMP, ABC and iron transporters”
e1“rice root upon AsIII exposure”
Claim · model-written summary
NO modulated rice stress-related genes and transcription factors during arsenic stress.
- Taxon
- riceOryza sativae2
- Treatment
- NOe1
- Gene
- CytP450, GSTs, GRXs, TFse0
- Outcome
- stress related genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress related genes such as CytP450, GSTs, GRXs, TFs”
e1“NO was found to modulate metal transporters particularly NIP, NRAMP, ABC and iron transporters, stress related genes such as CytP450, GSTs, GRXs, TFs”
e2“rice root upon AsIII exposure”
Claim · model-written summary
NO modulated rice genes of amino acid, hormone, signaling and secondary metabolism during arsenic stress.
- Taxon
- riceOryza sativae1
- Treatment
- NOe0
- Outcome
- amino acid, hormone(s), signaling and secondary metabolism genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO was found to modulate metal transporters particularly NIP, NRAMP, ABC and iron transporters, stress related genes such as CytP450, GSTs, GRXs, TFs, amino acid, hormone(s), signaling and secondary metabolism genes involved in As detoxification”
e1“rice root upon AsIII exposure”
Claim · model-written summary
NO changed rice jasmonic acid content during AsIII stress.
- Taxon
- riceOryza sativae1
- Treatment
- NOe0
- Outcome
- jasmonic acid (JA) contente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO-mediated change in jasmonic acid (JA) content during AsIII stress”
e1“rice root upon AsIII exposure”
Claim · model-written summary
The authors infer that NO reduces rice AsIII toxicity through detoxification regulatory networks and JA biosynthesis.
- Taxon
- riceOryza sativae1
- Treatment
- NOe0
- Outcome
- AsIII toxicitye0
- Qualifier
- modulating regulatory networks involved in As detoxification and JA biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO reduces AsIII toxicity through modulating regulatory networks involved in As detoxification and JA biosynthesis”
e1“rice root upon AsIII exposure”
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.