Abstract
The plant hormone jasmonate (JA) promotes the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins to relieve repression on diverse transcription factors (TFs) that execute JA responses. However, little is known about how combinatorial complexity among JAZ-TF interactions maintains control over myriad aspects of growth, development, reproduction, and immunity. We used loss-of-function mutations to define epistatic interactions within the core JA signaling pathway and to investigate the contribution of MYC TFs to JA responses in Arabidopsis thaliana. Constitutive JA signaling in a jaz quintuple mutant (jazQ) was largely eliminated by mutations that block JA synthesis or perception. Comparison of jazQ and a jazQ myc2 myc3 myc4 octuple mutant validated known functions of MYC2/3/4 in root growth, chlorophyll degradation, and susceptibility to the pathogen Pseudomonas syringae. We found that MYC TFs also control both the enhanced resistance of jazQ leaves to insect herbivory and restricted leaf growth of jazQ. Epistatic transcriptional profiles mirrored these phenotypes and further showed that triterpenoid biosynthetic and glucosinolate catabolic genes are up-regulated in jazQ independently of MYC TFs. Our study highlights the utility of genetic epistasis to unravel the complexities of JAZ-TF interactions and demonstrates that MYC TFs exert master control over a JAZ-repressible transcriptional hierarchy that governs growth-defense balance.
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Claims 6
Claim · model-written summary
Mutations blocking JA synthesis or perception largely eliminated constitutive JA signaling in jazQ.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Material
- jaz quintuple mutant (jazQ)e0
- Treatment
- mutations that block JA synthesis or perceptione0
- Outcome
- Constitutive JA signalinge0
- Direction
- eliminatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive JA signaling in a jaz quintuple mutant (jazQ) was largely eliminated by mutations that block JA synthesis or perception.”
e1“in Arabidopsis thaliana”
Claim · model-written summary
MYC2/3/4 affected root growth, chlorophyll degradation, and susceptibility to Pseudomonas syringae in the jazQ background.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- jazQ myc2 myc3 myc4 octuple mutante0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Gene
- MYC2/3/4e0
- Outcome
- root growth, chlorophyll degradation, and susceptibilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparison of jazQ and a jazQ myc2 myc3 myc4 octuple mutant validated known functions of MYC2/3/4 in root growth, chlorophyll degradation, and susceptibility to the pathogen Pseudomonas syringae.”
e1“in Arabidopsis thaliana”
Claim · model-written summary
MYC transcription factors controlled enhanced resistance of jazQ leaves to insect herbivory.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- jazQe0
- Organ / stage
- leavese0
- Gene
- MYC TFse0
- Outcome
- enhanced resistance of jazQ leaves to insect herbivorye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYC TFs also control both the enhanced resistance of jazQ leaves to insect herbivory and restricted leaf growth of jazQ.”
e1“in Arabidopsis thaliana”
Claim · model-written summary
MYC transcription factors controlled restricted leaf growth of jazQ.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- jazQe0
- Gene
- MYC TFse0
- Outcome
- restricted leaf growth of jazQe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYC TFs also control both the enhanced resistance of jazQ leaves to insect herbivory and restricted leaf growth of jazQ.”
e1“in Arabidopsis thaliana”
Claim · model-written summary
Triterpenoid biosynthetic genes were up-regulated in jazQ independently of MYC transcription factors.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- jazQe0
- Gene
- triterpenoid biosynthetice0
- Outcome
- triterpenoid biosynthetic and glucosinolate catabolic genese0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“triterpenoid biosynthetic and glucosinolate catabolic genes are up-regulated in jazQ independently of MYC TFs.”
e1“in Arabidopsis thaliana”
Claim · model-written summary
Glucosinolate catabolic genes were up-regulated in jazQ independently of MYC transcription factors.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- jazQe0
- Gene
- glucosinolate catabolic genese0
- Outcome
- glucosinolate catabolic genese0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“triterpenoid biosynthetic and glucosinolate catabolic genes are up-regulated in jazQ independently of MYC TFs.”
e1“in Arabidopsis thaliana”
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