Abstract
Drought and heat stress are major causes of lost plant crop yield. In the future, high levels of CO2, in combination of other abiotic stress factors, will become a novel source of stress. Little is known of the mechanisms involved in the acclimation responses of plants to this combination of abiotic stress factors, though it has been demonstrated that heat shock transcription factors (Hsfs) are involved in plant response to various abiotic stresses. In this study, we performed a genome-wide identification and a systematic analysis of genes in the Hsf gene family in Brassica napus. A total of 64 genes encoding Hsf proteins were identified and classified into 3 major classes: A, B and C. We found that, unlike in other eudicots, the A9 subclass is absent in rapeseed. Further gene structure analysis revealed a loss of the only intron in the DBD domain for BnaHsf63 and -64 within class C, which is evolutionarily conserved in all Hsf genes. Transcription profile results demonstrated that most BnaHsf family genes are upregulated by both drought and heat conditions, while some are responded to a high CO2 treatment. According to the combined RNA-seq and qRT-PCR analysis, the A1E/A4A/A7 subclasses were upregulated by both drought and heat treatments. Members in class C seemed to be predominantly induced only by drought. Among BnaHsf genes, the A2/A3/B2 subclasses were regulated by all three abiotic stresses. Members in A2/B2 subclasses were upregulated by drought and heat treatments, but were downregulated under high CO2 conditions. While the A3 subclass was upregulated by all the three abiotic stresses. Various stress-related cis-acting elements, enriched in promoter regions, were correlated with the transcriptional response of BnaHsfs to these abiotic stresses. Further study of these novel groups of multifunctional BnaHsf genes will improve our understanding of plant acclimation response to abiotic stresses, and may be useful for improving the abiotic stress resistance of crop varieties.
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Claims 11
Claim · model-written summary
64 Hsf genes were identified and assigned to classes A, B and C.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Gene
- Hsf proteinse0
- Outcome
- 64 genes encoding Hsf proteinse0
- Value
- 64 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“A total of 64 genes encoding Hsf proteins were identified and classified into 3 major classes: A, B and C.”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
The A9 subclass was absent in rapeseed.
- Taxon
- Brassica napusBrassica napust0
- Gene
- A9 subclasse0
- Outcome
- A9 subclass is absente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“unlike in other eudicots, the A9 subclass is absent in rapeseed”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
BnaHsf63 and -64 lost the DBD domain intron.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnaHsf63 and -64e0
- Outcome
- a loss of the only intron in the DBD domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a loss of the only intron in the DBD domain for BnaHsf63 and -64 within class C”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
Most BnaHsf genes were upregulated by drought and heat.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- drought and heat conditionse0
- Gene
- BnaHsf family genese0
- Outcome
- BnaHsf family genes are upregulatede0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most BnaHsf family genes are upregulated by both drought and heat conditions”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
Some BnaHsf genes responded to high CO2.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- high CO2 treatmente0
- Outcome
- responded to a high CO2 treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some are responded to a high CO2 treatment”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
A1E/A4A/A7 subclasses were upregulated by drought and heat.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- drought and heat treatmentse0
- Gene
- A1E/A4A/A7 subclassese0
- Outcome
- A1E/A4A/A7 subclasses were upregulatede0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the A1E/A4A/A7 subclasses were upregulated by both drought and heat treatments”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
Class C members were induced predominantly by drought.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- droughte0
- Gene
- class Ce0
- Outcome
- Members in class C seemed to be predominantly inducede0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Members in class C seemed to be predominantly induced only by drought”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
A2/B2 subclasses were upregulated by drought and heat.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- drought and heat treatmentse0
- Gene
- A2/B2 subclassese0
- Outcome
- Members in A2/B2 subclasses were upregulatede0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Members in A2/B2 subclasses were upregulated by drought and heat treatments”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
A2/B2 subclasses were downregulated by high CO2.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- high CO2 conditionse0
- Gene
- A2/B2 subclassese0
- Outcome
- Members in A2/B2 subclasses were upregulatede0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Members in A2/B2 subclasses were upregulated by drought and heat treatments, but were downregulated under high CO2 conditions”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
A3 subclass was upregulated by all three abiotic stresses.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- all the three abiotic stressese0
- Gene
- A3 subclasse0
- Outcome
- A3 subclass was upregulatede0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the A3 subclass was upregulated by all the three abiotic stresses”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
Claim · model-written summary
Promoter cis elements correlated with BnaHsf transcriptional responses.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnaHsfse0
- Outcome
- transcriptional response of BnaHsfse0
- Qualifier
- stress-related cis-acting elementse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Various stress-related cis-acting elements, enriched in promoter regions, were correlated with the transcriptional response of BnaHsfs to these abiotic stresses”
t0“Systematic Analysis of Hsf Family Genes in the Brassica napus Genome Reveals Novel Responses to Heat, Drought and High CO2 Stresses”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.