Abstract
To uncover the alleviation mechanism of quinclorac stress by salicylic acid (SA), leaf samples of Oryza sativa ssp. Japonica under quinclorac stress with and without SA pre-treatment were analyzed for transcriptional and proteomic profiling to determine the differentially expressed genes (DEGs) and proteins (DEPs), respectively. Results showed that quinclorac stress altered the expression of 2207 DEGs (1427 up-regulated, 780 down-regulated) and 147 DEPs (98 down-regulated, 49 up-regulated). These genes and proteins were enriched in glutathione (GSH) metabolism, porphyrin and chlorophyll metabolism, the biosynthesis of secondary metabolites, glyoxylate and dicarboxylate metabolism, and so on. It also influenced apetala2- ethylene-responsive element binding protein (AP2-EREBP) family, myeloblastosis (MYB) family and WRKY family transcription factors. After SA pre-treatment, 697 genes and 124 proteins were differentially expressed. Pathway analysis showed similar enrichments in GSH, glyoxylate and dicarboxylate metabolism. Transcription factors were distributed in basic helix-loop-helix (bHLH), MYB, Tify and WRKY families. Quantitative real-time PCR results revealed that quinclorac stress induced the expression of glutathion reductase (GR) genes (OsGR2, OsGR3), which was further pronounced by SA pre-treatment. Quinclorac stress further mediated the accumulation of acetaldehyde in rice, while SA enhanced the expression of OsALDH2B5 and OsALDH7 to accelerate the metabolism of herbicide quinclorac for the protection of rice. Correlation analysis between transcriptome and proteomics demonstrated that, under quinclorac stress, correlated proteins/genes were mainly involved in the inhibition of intermediate steps in the biosynthesis of chlorophyll. Other interesting proteins/genes and pathways regulated by herbicide quinclorac and modulated by SA pre-treatment were also discussed, based on the transcriptome and proteomics results.
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Claims 14
Claim · model-written summary
Quinclorac stress altered the expression of 2207 differentially expressed genes in rice leaves, 1427 up-regulated and 780 down-regulated.
- Taxon
- Oryza sativa ssp. Japonica
Oryza sativae0 - Treatment
- quinclorac stresse1
- Outcome
- DEGse1
- Direction
- alterede1
- Value
- 2207e1
- Qualifier
- 1427 up-regulated, 780 down-regulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“quinclorac stress altered the expression of 2207 DEGs (1427 up-regulated, 780 down-regulated) and 147 DEPs (98 down-regulated, 49 up-regulated)”
Claim · model-written summary
Quinclorac stress altered the abundance of 147 differentially expressed proteins in rice leaves, 98 down-regulated and 49 up-regulated.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Treatment
- quinclorac stresse1
- Outcome
- DEPse1
- Direction
- alterede1
- Value
- 147e1
- Qualifier
- 98 down-regulated, 49 up-regulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“quinclorac stress altered the expression of 2207 DEGs (1427 up-regulated, 780 down-regulated) and 147 DEPs (98 down-regulated, 49 up-regulated)”
Claim · model-written summary
The genes and proteins altered by quinclorac stress were enriched in glutathione metabolism, porphyrin and chlorophyll metabolism, secondary metabolite biosynthesis and glyoxylate and dicarboxylate metabolism.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Outcome
- enriched in glutathione (GSH) metabolism, porphyrin and chlorophyll metabolism, the biosynthesis of secondary metabolites, glyoxylate and dicarboxylate metabolisme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“These genes and proteins were enriched in glutathione (GSH) metabolism, porphyrin and chlorophyll metabolism, the biosynthesis of secondary metabolites, glyoxylate and dicarboxylate metabolism, and so on”
Claim · model-written summary
Quinclorac stress also influenced AP2-EREBP, MYB and WRKY family transcription factors.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Outcome
- influenced apetala2- ethylene-responsive element binding protein (AP2-EREBP) family, myeloblastosis (MYB) family and WRKY family transcription factorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“It also influenced apetala2- ethylene-responsive element binding protein (AP2-EREBP) family, myeloblastosis (MYB) family and WRKY family transcription factors”
Claim · model-written summary
After salicylic acid pre-treatment, 697 genes were differentially expressed in rice under quinclorac stress.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Treatment
- SA pre-treatmente1
- Outcome
- genese1
- Direction
- differentially expressede1
- Value
- 697e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“After SA pre-treatment, 697 genes and 124 proteins were differentially expressed”
Claim · model-written summary
After salicylic acid pre-treatment, 124 proteins were differentially expressed in rice under quinclorac stress.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Treatment
- SA pre-treatmente1
- Outcome
- proteinse1
- Direction
- differentially expressede1
- Value
- 124e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“After SA pre-treatment, 697 genes and 124 proteins were differentially expressed”
Claim · model-written summary
With SA pre-treatment, pathway analysis showed similar enrichments in glutathione, glyoxylate and dicarboxylate metabolism.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Outcome
- similar enrichments in GSH, glyoxylate and dicarboxylate metabolisme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“Pathway analysis showed similar enrichments in GSH, glyoxylate and dicarboxylate metabolism”
Claim · model-written summary
After SA pre-treatment, transcription factors were distributed in the bHLH, MYB, Tify and WRKY families.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Outcome
- Transcription factors were distributed in basic helix-loop-helix (bHLH), MYB, Tify and WRKY familiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“Transcription factors were distributed in basic helix-loop-helix (bHLH), MYB, Tify and WRKY families”
Claim · model-written summary
Quinclorac stress induced expression of the glutathione reductase genes OsGR2 and OsGR3 in rice, shown by quantitative real-time PCR.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Treatment
- quinclorac stresse1
- Gene
- OsGR2, OsGR3e1
- Outcome
- expression of glutathion reductase (GR) genese1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“quinclorac stress induced the expression of glutathion reductase (GR) genes (OsGR2, OsGR3), which was further pronounced by SA pre-treatment”
Claim · model-written summary
The quinclorac-induced expression of OsGR2 and OsGR3 was further pronounced by SA pre-treatment.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Treatment
- SA pre-treatmente1
- Gene
- OsGR2, OsGR3e1
- Outcome
- expression of glutathion reductase (GR) genese1
- Direction
- further pronouncede1
- Comparator
- quinclorac stresse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“quinclorac stress induced the expression of glutathion reductase (GR) genes (OsGR2, OsGR3), which was further pronounced by SA pre-treatment”
Claim · model-written summary
Quinclorac stress further mediated the accumulation of acetaldehyde in rice.
- Taxon
- riceOryza sativae1
- Treatment
- Quinclorac stresse1
- Outcome
- accumulation of acetaldehydee1
- Direction
- accumulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Quinclorac stress further mediated the accumulation of acetaldehyde in rice”
Claim · model-written summary
SA enhanced the expression of OsALDH2B5 and OsALDH7 in rice.
- Taxon
- riceOryza sativae1
- Treatment
- SAe1
- Gene
- OsALDH2B5 and OsALDH7e1
- Outcome
- expression of OsALDH2B5 and OsALDH7e1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“SA enhanced the expression of OsALDH2B5 and OsALDH7 to accelerate the metabolism of herbicide quinclorac for the protection of rice”
Claim · model-written summary
The authors propose that SA-enhanced OsALDH2B5 and OsALDH7 expression accelerates metabolism of the herbicide quinclorac and so protects rice.
- Taxon
- riceOryza sativae1
- Treatment
- SAe1
- Gene
- OsALDH2B5 and OsALDH7e1
- Outcome
- metabolism of herbicide quinclorace1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“SA enhanced the expression of OsALDH2B5 and OsALDH7 to accelerate the metabolism of herbicide quinclorac for the protection of rice”
Claim · model-written summary
Under quinclorac stress, correlated proteins and genes were mainly involved in inhibition of intermediate steps of chlorophyll biosynthesis.
- Taxon
- Oryza sativa ssp. JaponicaOryza sativae0
- Treatment
- quinclorac stresse1
- Outcome
- inhibition of intermediate steps in the biosynthesis of chlorophylle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa ssp. Japonica”
e1“under quinclorac stress, correlated proteins/genes were mainly involved in the inhibition of intermediate steps in the biosynthesis of chlorophyll”
The same questions in other papers
salicylic acid · molecular: expression · Oryza sativa (rice)Positive and negative results
27 papers in the same direction as this paper · 3 in the opposite direction
- positive
- no effect
- no effect
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.